Core B: Animal Core and Germ-Free Mouse Core

核心B：动物核心和无菌小鼠核心

基本信息

批准号：
10588796
负责人：
Lucy Hoffmann Kennedy
金额：
$ 11.44万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-02-01 至 2028-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10588796
关键词：
Accreditation Adherence American Animal Experimentation Animals Behavior Blood Body Weight Caring Certification Consultations Derivation procedure Diabetes Mellitus Diet Dose Ensure Environmental Monitoring Experimental Designs Fasting Feces Film Genetic Engineering Germ-Free Glucose Gnotobiotic Goals Guidelines Health Health Status Housing IACUC Inbred BALB C Mice Institution Laboratories Laboratory Animal Medicine Laboratory mice Maintenance Michigan Modeling Monitor Mus Obesity Personal Satisfaction Phenotype Play Process Protocols documentation Quarantine Reproducibility Research Research Personnel Role Services Techniques Technology Training United States Department of Agriculture Universities Urine Veterinarians Workplace animal care college flexibility germ free condition metabolic phenotype pathogen sample collection segregation ventilation

项目摘要

Project Summary / Abstract – Animal Care and Germ-Free Mouse Core The Animal Care and Germ-Free Mouse Core of the Michigan MPMOD will play a central role in the management of animal flow through the Center’s MPB Phenotyping Core from initial acquisition to final disposition of imported mice. Both the Core Director and Associate Director are veterinarians and diplomates of the American College of Laboratory Animal Medicine. Specific functions of the Animal Care and Germ-Free Mouse Core include the coordination of mouse importation, quarantine, and environmental testing for a panel of murine pathogens, as well as providing housing, husbandry, and veterinary care of mice in accordance with the MPMOD (Administrative Core) guidelines. Research services such as hands-on technical support and germ-free technology and expertise will be unique components of our Core. Germ-free C57BL/6J, C57BL/6NCrl, BALB/c, and Swiss-Webster mice as well as several genetically engineered strains will be available to MPMOD investigators. In addition, investigators may import germ-free mice from other institutions or request rederivation of new germ-free strains. Experimental manipulations available for germ-free and SPF mice include routine treatments, sample collections, and in-house analysis (blood, feces, urine, etc.), training, and experimental design consultation, among others. Goals of the Animal Care and Germ-Free Mouse Core are to provide the highest level of health and well-being as well as veterinary expertise for all research animals in our USDA-certified and AAALAC-accredited facilities. All Core services will be performed under University of Michigan IACUC approved protocols. All SPF mice will be housed in Allentown ventilated racks. Germ-free or gnotobiotic mice will be maintained in flexible-film isolators or Tecniplast Isocage racks. Barrier conditions including cage changing in vertical laminar flow changing stations, strict adherence to microisolator techniques, and use of isolated rooms for colony segregation will assure maintenance of pathogen-free animals. The Animal Core will also provide oversight for technical support such as dose administration, obtaining body weights, fasting, glucose levels, providing and monitoring special diets, and transfer of mice to and from ULAM housing to the laboratories of the MPB Phenotyping Core.

项目摘要 /摘要 - 动物护理和无细菌鼠标核心密歇根州mpmod的动物护理和无菌小鼠核心将在从最初获取到最终处置进口小鼠。核心董事和副主任都是兽医和文凭美国实验室动物医学学院。动物护理和无菌的特定功能鼠标核心包括小鼠进口，隔离和面板环境测试的协调根据鼠病原体以及根据鼠标提供住房，饲养和兽医护理 MPMOD（行政核心）指南。研究服务，例如动手技术支持和无菌技术和专业知识将是我们核心的独特组成部分。无细菌C57BL/6J， C57BL/6NCRL，BALB/C和Swiss-Webster小鼠以及几种基因工程菌株将是可用于mpmod调查人员。此外，调查人员可能会从其他机构进口无菌小鼠或要求对新的无菌菌株进行细化。可用于无菌和SPF的实验操作小鼠包括常规治疗，样本收集和内部分析（血液，粪便，尿液等），训练，和实验设计咨询等。动物护理和无菌鼠标核心的目标为所有研究动物提供最高水平的健康和福祉以及兽医专业知识在我们的USDA认证和AAALAC认可的设施中。所有核心服务将在大学的下进行密歇根州IACUC批准的方案。所有SPF鼠标都将放置在Allentown通风架上。无菌或 Gnotobiotic小鼠将保持在柔性膜隔离器或Tecniplast Isocage架中。障碍条件包括垂直层流换台站的笼子改变，严格遵守微发射器技术，并使用孤立的房间进行菌落隔离将确保维持无病原体动物。这动物核心还将为技术支持（例如剂量给药）提供监督，获得身体重量，禁食，葡萄糖水平，提供和监测特殊饮食以及小鼠往返Ulam的转移壳体到MPB表型核心的实验室。