How bacterial SMC complexes organize chromosomes (Equipment Supplement)

细菌SMC复合体如何组织染色体（设备补充）

• 批准号: 10580926
• 负责人: Xindan Wang
• 金额: $ 2.44万
• 依托单位: TRUSTEES OF INDIANA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10580926
• 关键词: Administrative Supplement; Bacteria; Biochemical; Cell Culture Techniques; Cell Cycle; Cells; Chromosome Segregation; Chromosomes; Complex; Cytology; Equipment; Funding; Genetic; Goals; Human; Incubators; Maintenance; Molecular; New Brunswick; Organism; Research; System; condensin; experimental study; genome integrity; parent grant

Project Summary The primary goal of our funded research (1R01GM141242) is to understand how the chromosome is organized and segregated during the cell cycle. We focus on the molecular mechanisms of SMC condensin complex, which is a key player for chromosome dynamics from bacteria to humans. Our projects combine genetic, molecular, cytological, and biochemical approaches. All of these approaches require that we grow the bacterial cells to the desired experimental conditions. In this application for Administrative Supplements for Equipment, I am requesting the New Brunswick Innova 44R system, which will allow us to continue growing cells to perform the experiments proposed in the parent grant.

项目概要 我们资助的研究 (1R01GM141242) 的主要目标是了解染色体是如何组织的 并在细胞周期中分离。我们重点研究SMC凝缩蛋白复合物的分子机制， 是从细菌到人类染色体动力学的关键参与者。我们的项目结合了遗传、分子、 细胞学和生化方法。所有这些方法都要求我们将细菌细胞培养到 所需的实验条件。在这份设备行政补充申请中，我是 请求新不伦瑞克省 Innova 44R 系统，这将使我们能够继续培养细胞以执行 家长资助中提出的实验。