Synaptic organization and plasticity of the input from the amygdala to the gustatory cortex

从杏仁核到味觉皮层的输入的突触组织和可塑性

基本信息

批准号：
10577815
负责人：
Alfredo Fontanini
金额：
$ 33.76万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-03-01 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10577815
关键词：
Acute Affect Affective Amygdaloid structure Anatomy Anesthesia procedures Anhedonia Animals Behavioral Cell Nucleus Communities Complex Data Detection Development Dimensions Electrophysiology (science)Experimental Designs Genetic Human Knowledge Laboratories Learning Lesion Medial Mental disorders Modeling Molecular Nature Neurons Neurosciences Perception Play Population Preparation Process Property Public Health Publishing Reporting Research Rodent Role Sensory Slice Stimulus Synapses Synaptic Transmission Synaptic plasticity Taste Perception Taste aversion Testing Thalamic structure Therapeutic Intervention Training Work experimental study hedonic hippocampal pyramidal neuron in vivo nerve supply nervous system disorder novel novel therapeutic intervention optogenetics patch clamp pharmacologic postsynaptic preservation response sensory integration sensory mechanism sensory stimulus taste stimuli taste system therapeutic development tool transmission process

项目摘要

Summary The perception of taste is a complex process that includes the chemosensory detection of a stimulus as well as information regarding its hedonic value, whether a stimulus is pleasant or unpleasant. The gustatory cortex is thought to be a central component of the taste system, and is hypothesized to integrate both of chemosensory and affective dimensions of taste stimuli. However, to date, the neuronal and circuit mechanisms involved in this process remain unclear. In this proposal, we will fill a gap in the current understanding of circuit underpinning for taste by investigating the synaptic mechanisms underlying the integration of thalamocortical and amygdalocortical inputs to the primary gustatory cortex. The thalamocortical projection from the taste thalamus provides the gateway for chemosensory information to the gustatory cortex, while the basolateral nucleus of the amygdala has been identified as a major contributor of hedonic information regarding taste stimuli. Taking advantage of cutting-edge experimental approaches for circuit and synaptic analysis, and of a well-established learning paradigm, conditioned taste aversion, that preserves the chemosensory identity, but alters a stimulus affective dimension, we will determine how sensory and hedonic components of taste are integrated in the gustatory cortical circuit. The approach we propose will begin to bridge the gap between the extensive behavioral/ pharmacological and cellular/molecular work that has significantly advanced the field, but without information about underlying synaptic and circuit mechanisms remains difficult to reconcile in an overarching framework. In addition, our study has important implication for public health. Many neurological and psychiatric disorders are associated with altered sensory perception, or by anhedonia. Taste perception in human and rodents is characterized by sensory and affective dimensions, therefore it provides a unique model for understanding common mechanism of sensory processing, as well as for investigating the circuit underpinning for the integration of sensory stimuli with their hedonic value. Therefore, results from our current study have the potential of identifying novel targets for the development of therapeutic intervention.

概括口味的感知是一个复杂的过程，包括刺激的化学感应检测以及有关其享乐价值的信息，无论刺激是令人愉悦的还是不愉快的。味性皮质是被认为是味觉系统的核心组成部分，并被认为可以整合两种化学感应和味觉刺激的情感维度。但是，迄今为止，涉及的神经元和电路机制过程尚不清楚。在此提案中，我们将在当前对基础电路的理解中填补空白通过研究丘脑皮质和整合的突触机制来品尝杏仁核皮质输入到主要的味道皮层。来自味道丘脑的丘脑皮层投影为味觉皮层提供化学感应信息的门户，而基底外侧核杏仁核已被确定为享乐信息有关刺激的主要贡献者。服用尖端的实验方法的优势，用于电路和突触分析，并建立了良好的实验方法学习范式，条件性的口味厌恶，保留化学感知身份，但改变了刺激情感维度，我们将确定感官和享乐分量如何整合到味道皮质回路。我们提出的方法将开始弥合广泛的行为/ 药理学和细胞/分子工作已经显着推进了领域，但没有信息关于潜在的突触和电路机制在总体框架中仍然很难和解。此外，我们的研究对公共卫生具有重要意义。许多神经和精神病与改变的感觉感知或抗抗激素有关。人类和啮齿动物的味觉感知是以感觉和情感维度为特征，因此为理解提供了独特的模型感觉处理的常见机制，以及研究基础的电路感觉刺激与其享乐值的整合。因此，我们当前研究的结果具有潜力确定用于发展治疗干预措施的新目标。