Metabolic Phenotyping Core

代谢表型核心

• 批准号: 10571892
• 负责人: TIMOTHY M GRIFFIN
• 金额: $ 30.06万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-02-05 至 2026-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10571892
• 关键词: Address; Advisory Committees; Aging; Area; Autoimmune Diseases; Bioinformatics; Biological; Biological Assay; Cardiovascular Diseases; Cell Respiration; Cells; Cellular Metabolic Process; Centers of Research Excellence; Clinical Trials; Complex; Consultations; Data; Data Analyses; Decision Making; Degenerative polyarthritis; Development; Diet; Disease; Doctor of Philosophy; Ensure; Environment; Equipment; Evaluation; Experimental Designs; Fostering; Functional disorder; Funding; Future; Genus Hippocampus; Goals; Growth; Interdisciplinary Study; Isotopes; Leadership; Malignant Neoplasms; Mass Fragmentography; Measurement; Mediating; Mentors; Mentorship; Metabolic; Metabolism; Metformin; Methodology; Methods; Mitochondria; Modeling; Mus; Obese Mice; Oklahoma; Pathology; Phase; Physiology; Positioning Attribute; Procedures; Process; Regulation; Research; Research Design; Research Personnel; Research Support; Resources; Role; Services; Standardization; Structure; Technical Expertise; Techniques; Tissues; Tracer; Training; analytical method; comparative; design; exercise intervention; experience; follow-up; in vivo; instrument; instrumentation; interest; meetings; member; metabolic abnormality assessment; metabolic phenotype; mouse development; multidisciplinary; new technology; novel; operation; programs; protein degradation; skills; stable isotope; sustainable resource

The goal of the Center for Cellular Metabolism Research in Oklahoma (CMRO) Phase I COBRE proposal is to cultivate novel multidisciplinary research focusing on metabolism. As part of this overall goal, we created the Metabolic Phenotyping Core to facilitate the transition of our COBRE investigators to independent R01 funding and to develop metabolic research in Oklahoma. The projects proposed by the COBRE investigators seek to understand the mechanisms by which cellular metabolic processes drive or result from cardiovascular disease, autoimmune disease, and cancer. Because cellular metabolism is complex and poses many biological and technical challenges, the Core will provide a team of highly-trained directors and PhD-level scientific staff, access to state-of-the-art instruments that are carefully maintained, and mentored training in experimental design, implementation, and interpretation of metabolic data. The Core addresses three critical needs to support our COBRE investigators: 1) assessment of cellular respiration and mitochondrial function, 2) isotopic metabolic tracer analyses, and 3) metabolite-based metabolic profiling and bioinformatic analyses. To meet these needs, our specific aims are to: 1) provide expertise with study design, assay optimization, data analysis, and interpretation of results, as well as evaluate and develop new methodologies for in vivo metabolic phenotyping, and 2) provide COBRE investigators access to centralized equipment and appropriate scientific and technical training to facilitate the development of new expertise in cellular metabolic assessment. To support the COBRE Investigators, the Core will provide instrumentation for cellular respiration and mitochondrial function (e.g. Seahorse, Oroboros, and EPR), metabolite tracer analysis (LC-MS/MS), and metabolic profiling (GC-MS and GC-MS/MS). The Core will be directed by Dr. Timothy Griffin and Co-directed by Dr. Benjamin Miller. Both directors have over 20 years of experience in studies of metabolism and previous experience in leadership positions. The Core has a proposed structure that facilitates clearly-defined roles of support staff as well as transparent decision-making processes. Through this structure, the Core will foster a mentoring and collaborative culture, promote an idea- and method-sharing environment, and contribute to the establishment of a sustainable resource for metabolic research for COBRE Investigators as well as other researchers in Oklahoma.

俄克拉荷马州（CMRO）I期COBRE建议的细胞代谢研究中心的目标是 培养针对代谢的新型多学科研究。作为整个目标的一部分，我们创建了 代谢表型核心，以促进我们的毛绒研究人员向独立R01资金过渡 并在俄克拉荷马州进行代谢研究。毛绒调查人员提出的项目寻求 了解细胞代谢过程驱动或由心血管疾病引起的机制， 自身免疫性疾病和癌症。因为细胞代谢很复杂，并且构成了许多生物学和 技术挑战，核心将为训练有素的董事和博士学位科学人员提供一个团队，访问 要精心维护的最先进的工具，并在实验设计方面进行了指导的培训， 实施和代谢数据的解释。核心解决了支持我们的三个关键需求 鞋垫研究人员：1）评估细胞呼吸和线粒体功能，2）同位素代谢 示踪剂分析和3）基于代谢物的代谢分析和生物信息学分析。为了满足这些需求， 我们的具体目的是：1）提供研究设计，测定优化，数据分析和 解释结果，并评估和开发用于体内代谢表型的新方法， 2）提供毛to研究者使用集中设备以及适当的科学和技术 培训以促进细胞代谢评估方面的新专业知识的发展。支持毛绒 研究者，核心将提供仪器的细胞呼吸和线粒体功能（例如， Seahorse，Oroboros和EPR），代谢物示踪剂分析（LC-MS/MS）以及代谢分析（GC-MS和GC-MS GC-MS/MS）。核心将由蒂莫西·格里芬（Timothy Griffin）博士执导，并由本杰明·米勒（Benjamin Miller）博士共同执导。两个都 董事在代谢研究和以前领导方面的经验方面拥有超过20年的经验 位置。核心具有拟议的结构，可促进支持人员明确定义的角色 透明的决策过程。通过这种结构，核心将促进指导和协作 文化，促进思想和方法共享环境，并为建立可持续性做出贡献 毛毛调查人员以及俄克拉荷马州的其他研究人员的代谢研究资源。