Multi-omics profiling of individual exosomes for their origin-tracing, biomarker discovery, and biological function characterization

对单个外泌体进行多组学分析，以进行起源追踪、生物标志物发现和生物功能表征

• 批准号: 10572470
• 负责人: Yuchao Chen
• 金额: $ 0.65万
• 依托单位: WELLSIM BIOMEDICAL TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-01 至 2022-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10572470
• 关键词: Address; Algorithms; Antibodies; Bar Codes; Biogenesis; Bioinformatics; Biological; Biological Assay; Biological Markers; Biological Process; Biomedical Technology; Blood specimen; Communities; DNA Sequence; Data; Detection; Disease; Early Diagnosis; Emulsions; Encapsulated; Expression Profiling; Gene Expression Profiling; Goals; Heterogeneity; High-Throughput DNA Sequencing; Human Cell Line; Individual; Label; Membrane Proteins; Messenger RNA; Methodology; Methods; Microfluidic Microchips; Modeling; Nucleic Acids; Oligonucleotides; Outcome; Performance; Phase; Population; Preparation; Prognosis; Proteins; Proteomics; RNA; Research; Resolution; Sampling; Small Business Innovation Research Grant; Speed; System; Techniques; Technology; Tissues; Untranslated RNA; Variant; base; biomarker discovery; disease diagnosis; exosome; extracellular vesicles; imaging modality; improved; individual variation; innovation; insight; interest; multiple omics; nanolitre; next generation sequencing; particle; protein profiling; targeted treatment; technological innovation; tool; transcriptomics

PROJECT SUMMARY / ABSTRACT Exosomes, the small extracellular vesicles (30-200 nm), are highly heterogeneous in biofluids. However, traditional bulk-level analysis approaches fail to represent their individual variations. A technique for multi-omics profiling of exosomes at single-particle resolution is of great interest to the exosome research field, but has yet to be developed. WellSIM proposes to develop and validate a method for multi-omics profiling of individual exosomes based on our exosome isolation system (EXODUS), a droplet-based microfluidic device (EXOSeq), and next-generation sequencing (NGS). Our proposed technique will be the first integrative proteomic and transcriptomic profiling of exosomes at the single-particle level, offering high-resolution multi-dimensional biological insights for exosome study and application.

项目概要/摘要 外泌体是一种小的细胞外囊泡（30-200 nm），在生物体液中具有高度异质性。然而， 传统的批量分析方法无法代表其个体差异。多组学技术 单颗粒分辨率的外泌体分析引起了外泌体研究领域的极大兴趣，但尚未取得进展 待开发。 WellSIM 提议开发并验证一种对个体进行多组学分析的方法 基于我们的外泌体分离系统（EXODUS）的外泌体，一种基于液滴的微流体装置（EXOSeq）， 和下一代测序（NGS）。我们提出的技术将是第一个综合蛋白质组学和 单颗粒水平的外泌体转录组分析，提供高分辨率多维 外泌体研究和应用的生物学见解。