Animal Models of Disease Core

疾病核心动物模型

基本信息

批准号：
10091426
负责人：
WENHAO XU
金额：
$ 6.13万
依托单位：
UNIVERSITY OF VIRGINIA
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-09-16 至 2022-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10091426
关键词：
Adopted Advisory Committees Affect Animal Cancer Model Animal Disease Models Animal Model Animals Archives Area Biological Sciences Biometry Breast CRISPR/Cas technology Cancer Biology Cancer Center Cancer Center Support Grant Cancer Model Charge Collection Colorectal Communities Consultations Country Cryopreservation Cyclotrons Databases Derivation procedure Disease Drug resistance Educational workshop Endometrial Epigenetic Process Event Evolution Experimental Designs Experimental Models Foxes Funding Generations Genetic Genetic Engineering Genetically Engineered Mouse Genome engineering Goals Grant Growth Head and Neck Cancer Human Image Imaging technology Immune system Infrastructure Kidney Knock-in Mouse Knockout Mice Laboratories Leadership Libraries Lung Magnetic Resonance Imaging Malignant Neoplasms Malignant neoplasm of ovary Malignant neoplasm of pancreas Malignant neoplasm of urinary bladder Measures Microscopy Modeling Molecular Mus Mutation National Center for Research Resources Neoplasm Metastasis Online Systems Organ Pathway interactions Physiological Positron-Emission Tomography Price Process Property Prostate Research Research Personnel Resource Sharing Services Site Specimen Speed Study models System Technology Therapeutic Time Tissues Training Ultrasonography United States National Institutes of Health University of Virginia Cancer Center Xenograft Model biobank biological research cancer cell clinically relevant cohort comparative cost data acquisition equipment acquisition fluorescence imaging genome editing imaging capabilities imaging program improved in vivo monitoring instrument knockout gene luminescence medical schools melanoma member model design molecular imaging mouse model new technology novel therapeutics patient derived xenograft model pre-clinical research facility service providers spectroscopic imaging technology development tool tumor tumor growth tumor microenvironment tumor progression tumor xenograft

项目摘要

ANIMAL MODEL OF DISEASE CORE – PROJECT SUMMARY/ABSTRACT The Animal Model of Disease Core (AMDC) is a comprehensive shared resource that has served UVA Cancer Center researchers' needs for more than two decades. Mice are one of the most widely used animal models for cancer. While cancer researchers have successfully studied the molecular and cellular properties of cancer cells propagated in culture, increasingly mouse models of cancer provide a more physiologically relevant opportunity to investigate the interplay of cancer cells, the tumor microenvironment, and the host immune system. Mouse models provide opportunities to study the evolution of cancer progression from first mutations to metastasis, and the unique opportunity to assess the genetic and epigenetic events correlated with acquisition of drug resistance. Following guidance from the UVACC leadership, the AMDC was formed by integrating two pre-existing shared resources: the Genetically Engineered Murine Model (GEMM) Core, and the Preclinical Tumor Assessment and Imaging Core, which consisted of the Tumor Xenograft Model (TXM) and Molecular Imagining Core (MIC) sections. This merger was conducted with the goal of better integrating cancer animal model creation, analysis and imaging. The AMDC integrates genome engineering, tumor xenografting, and imaging technologies to efficiently produce and analyze cancer animal models for UVA Cancer Center investigators. Since the last CCSG renewal, the AMDC has made great strides in novel technology development and state- of-art instrument acquisition. The AMDC swiftly adopted the Crispr-Cas9 technology for genome editing which enables cancer animal model generation at an unprecedented speed with significantly lowered cost. The AMDC also greatly expanded their library of patient derived xenograft (PDX) tumor models of ovarian cancer, head and neck cancers, and prostate and kidney GU cancers. The ultimate goal of these PDX efforts is to generate several lines of highly clinically relevant cancer models to be made widely available to the UVA community of cancer investigators. The AMDC has upgraded its PET imaging program with the addition of a cyclotron, which was funded by an NIH-NCRR high end instrument grant. In 2011, the cyclotron was installed in the Life Sciences Annex (built with funds from UVA SOM) adjoining the Snyder Building in the Fontaine Research Park. The AMDC is administered under ORCA, led by Dr. Jay W. Fox, the UVA Cancer Center Associate Director for Laboratory Infrastructure. AMDC staff routinely interact with other Cancer Center shared resources in particular the Biomolecular Analysis Facility, Biostatistics, Advanced Microscopy Facility, and Biorepository and Tissue Research Facilities. The interactions with other shared resource facilities on behalf of AMDC users help make the AMDC a one-stop service provider. Many of the AMDC users are UVA Cancer Center members who benefit from a co-pay that reduces their charges. The AMDC also is supported by the School of Medicine, which further lowers the cost for the Cancer Center users. The AMDC is reviewed annually by the Cancer Center leadership and the School of Medicine Research Advisory Committee to determine the level of subsidy required to maintain a revenue neutral core. The AMDC is competitive in pricing, rapid in service turnout time, and provides guarantees matching or exceeding the standards set up by comparative shared resources around the country.

疾病核心动物模型 - 项目摘要/摘要疾病核心动物模型（AMDC）是一种全面的共享资源，已为UVA提供服务癌症中心研究人员的需求超过二十年。癌症的模型。在培养中传播的癌细胞，越来越多的小鼠癌症模型提供了更加生理的。研究癌细胞解释，肿瘤微观事业和宿主的相关机会统一免疫系统。转移的突变，以及评估遗传和事件相关的独特机会随着耐药性的获取。在UVACC领导层的指导下，AMDC是通过整合两个预陈述而形成的共享资源：基因工程的鼠模型（GEMM）核心和临床前肿瘤评估和成像核心由肿瘤武器模型（TXM）和分子想象组成核心（MIC）部分进行合并，目的是更好地整合癌症动物模型创建，分析和成像。有效生产和分析UVA癌症研究者的癌症动物模型的技术。自上次CCSG续订以来，AMDC在新的技术发展和国家 - 国家取得了长足的进步 AMDC Swiftery的ART仪器采用了CRISPR-CAS9技术用于基因组编辑使癌症动物模型以未经预言的速度显着降低 AMDC还大大扩展了其卵巢癌的患者衍生异种移植（PDX）肿瘤模型，头部癌症，前列腺和肾脏癌症的最终目标是生成高度临床上相关的癌症模型的依据，以使UVA无限癌症调查人员的社区。 Cyclotron在2011年由NIH-NCRR高端仪器资助。在Life Sciences Annex（由UVA SOM的资金建造）与Fontaine的Snyder建筑物相邻研究公园。 AMDC由UVA癌症中心协会协会Jay W. Fox博士领导的Underder Orca管理实验室基础设施总监AMDC工作人员常规特别是生物分子分析设施，生物统计学，晚期显微镜设施和生物座和组织研究设施。帮助使AMDC成为一站式服务提供商。许多AMDC用户是UVA癌症中心成员他们的收费也得到了医学院的支持，这进一步降低了费用。癌症中心的使用者每年都会审查AMDC 医学研究咨询委员会确定维持收入所需的补贴级别中性核心。通过比较共享在全国各地共享的标准匹配或提取标准。