Laboratory and Analytical Core

实验室和分析核心

• 批准号: 10242022
• 负责人: TORE EID
• 金额: $ 35.61万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-30 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10242022
• 关键词: Animals; Benzaldehyde; Biological; Biological Assay; Biological Markers; Biological Specimen Banks; Chemicals; Cherry - dietary; Cinnamon - dietary; Clinical; Collaborations; Communication; Consultations; Cotinine; Data; Databases; Dose; Equipment; Experimental Designs; Exposure to; Feedback; Freezing; Funding; Genus Vanilla; Glycyrrhizic Acid; Human; Human Resources; Individual; Infrastructure; Laboratories; Liquid substance; Measurement; Measures; Menthol; Methodology; Methods; Nicotine; Outcome; Outcome Study; Pathologist; Pilot Projects; Quality Control; Research; Research Personnel; Research Support; Resource Sharing; Resources; Risk; Route; Saccharin; Sampling; Science; Services; Site; Source; Sweetening Agents; Technology; Time; Tobacco; Tobacco Dependence; Tobacco use; Translating; United States National Institutes of Health; Variant; addiction; behavior influence; biobank; cinnamic aldehyde; cost; data sharing; experience; experimental arm; flexibility; interest; menthol glucuronide; nicotine exposure; repository; response; tobacco exposure; tobacco products; vanillin; vapor

ABSTRACT – LABORATORY AND ANALYTICAL CORE The proposed projects of this application are concerned with reducing tobacco addiction through better understanding of how flavors and sweeteners influence behavior and addiction related to nicotine and tobacco. Research strategies include administration, by various routes, of nicotine alone, and nicotine in conjunction with other constituents, including menthol, flavors and sweeteners, followed by assessments of responses to nicotine. Optimal interpretation of study outcomes requires that variations in exposures to nicotine and/or flavor and sweetener constituents across individuals and experimental arms are considered. The primary objectives of the analytical laboratory core will therefore be to: 1) accurately identify and measure nicotine, flavors and sweeteners in tobacco products, and vapors produced from them; and 2) to provide objective estimates of exposures to such products by quantifying levels of nicotine, nicotine metabolites, and other constituents in biological fluids. The core will directly support all three projects and pilots of this proposal and will also be available to other tobacco research centers, including Rapid Response Projects announced by the FDA-CTP, on a cost reimbursement basis. Furthermore, the core will be able to set up other assays as mandated by the evolving needs of the center. To facilitate collaborations across other tobacco research centers, the core will generate a database of selected tobacco product constituents and biomarkers of exposure. Finally, all biological samples obtained in this Center will be maintained on site, in a frozen sample repository. The core will be led by Dr. Julie Zimmerman, who has considerable experience with chemical analysis of tobacco products, and Dr. Tore Eid, a clinical pathologist and neuroscientist who directed the core laboratory of the current NIH-funded Yale Tobacco Center of Regulatory Science (TCORS), and is an expert in chemical analysis of biological samples.

摘要 - 实验室和分析核心 本应用程序的拟议项目涉及通过更好的减少烟草成瘾 了解口味和甜味剂如何影响与尼古丁和烟草有关的行为和成瘾。 研究策略包括通过各种途径，单独的尼古丁和尼古丁的管理策略 与其他构成，包括精神病，口味和甜味剂，然后评估对 尼古丁。对研究结果的最佳解释要求对尼古丁和/或风味的暴露量变化 甜味剂构成了个体，并且考虑了实验臂。主要目标 因此，分析实验室核心将是：1）准确识别和测量尼古丁，口味和 烟草产品中的甜味剂和从中产生的蒸气； 2）提供客观估计 通过量化尼古丁，尼古丁代谢产物和其他构成的水平来暴露于此类产品 生物流体。核心将直接支持该提案的所有三个项目和飞行员，也将是 可用于其他烟草研究中心，包括FDA-CTP宣布的快速响应项目， 以成本报销为基础。此外，核心将能够按照 中心不断发展的需求。为了促进其他烟草研究中心的合作，核心将 生成选择的烟草产品结构和暴露生物标志物的数据库。最后，一切 在该中心获得的生物样品将在冷冻样品存储库中维持在现场。核心 将由朱莉·齐默尔曼（Julie Zimmerman）博士领导，他在烟草化学分析方面拥有丰富的经验 产品和临床病理学家和神经科学家Tore Eid博士指导了该核心实验室 当前由NIH资助的耶鲁大学烟草科学中心（TCORS），是化学专家 分析生物样品。