Metabolomics Core

代谢组学核心

基本信息

批准号：
10241902
负责人：
Costas Andreas Lyssiotis
金额：
$ 26.08万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10241902
关键词：
Address Allogenic Amino Acids Bile Acids Bioenergetics Bioinformatics Biological Assay Biostatistics Core Blood specimen Butyrates Carbohydrates Carbon Cells Clinical Trials Custom Data Data Analyses Data Collection Development Disease Enrollment Ensure Epithelial Epithelial Cells Feces Gastrointestinal tract structure Genetic Diseases Genus Hippocampus Glucose Gnotobiotic Graph Hematopoietic Neoplasms Hematopoietic Stem Cell Transplantation Human Incidence Informatics Label Laboratories Maintenance Malignant Neoplasms Mass Spectrum Analysis Measures Metabolic Metabolic Pathway Metabolism Methodology Methods Morbidity - disease rate Mus Nature Nitrogen Nutrient Organism Pathway interactions Patients Pharmaceutical Preparations Plasma Population Production Program Research Project Grants Property Research Project Grants Research Proposals Research Support Resources Safety Severities Starch Statistical Data Interpretation Technology Therapeutic Tissues Toxic effect Tracer Volatile Fatty Acids assay development base bile acid metabolism chronic infection experience gastrointestinal graft vs host disease gut microbiome host microbiome in vivo Model insight large datasets lipidome metabolome metabolomics microbial microbial community microbiome microbiome analysis nutrient metabolism pharmacodynamic biomarker prevent programs stable isotope stool sample uptake

Address Allogenic Amino Acids Bile Acids Bioenergetics Bioinformatics Biological Assay Biostatistics Core Blood specimen Butyrates Carbohydrates Carbon Cells Clinical Trials Custom Data Data Analyses Data Collection Development Disease Enrollment Ensure Epithelial Epithelial Cells Feces Gastrointestinal tract structure Genetic Diseases Genus Hippocampus Glucose Gnotobiotic Graph Hematopoietic Neoplasms Hematopoietic Stem Cell Transplantation Human Incidence Informatics Label Laboratories Maintenance Malignant Neoplasms Mass Spectrum Analysis Measures Metabolic Metabolic Pathway Metabolism Methodology Methods Morbidity - disease rate Mus Nature Nitrogen Nutrient Organism Pathway interactions Patients Pharmaceutical Preparations Plasma Population Production Program Research Project Grants Property Research Project Grants Research Proposals Research Support Resources Safety Severities Starch Statistical Data Interpretation Technology Therapeutic Tissues Toxic effect Tracer Volatile Fatty Acids assay development base bile acid metabolism chronic infection experience gastrointestinal graft vs host disease gut microbiome host microbiome in vivo Model insight large datasets lipidome metabolome metabolomics microbial microbial community microbiome microbiome analysis nutrient metabolism pharmacodynamic biomarker prevent programs stable isotope stool sample uptake

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项目摘要

PROJECT SUMMARY/ABSTRACT – CORE B Allogenic hematopoietic stem cell transplant (allo-HCT) can provide a cure for chronic infections, genetic diseases, or cancers of the blood. However, its therapeutic utility is limited by associated toxicities resulting from graft versus host disease (GVHD). Among the most prominent morbidities of GVHD is damage to the gastrointestinal tract. Recent results from our groups have revealed that the severity of GVHD is impacted by the nature and metabolic properties of the gut microbiome. Further, we found that promotion of microbial communities that facilitate the metabolism of starch into butyrate and reprogram bile acid metabolism of the host ameliorate or even prevent GVDH following BMT. The measure and quantitation of metabolism is essential to address the associated experimental aims across the research proposals. The purpose of the Metabolomics core is to support the research projects that build on these studies to detect and quantify metabolites from these pathways in stool from mice, bacterial populations, human epithelial cells, and longitudinal stool samples of patients in the associated clinical trial. Further, this core will also provide global insights into host and microbiome metabolism using stable isotope tracing technologies and untargeted metabolome-wide profiling. By centralizing the development, maintenance, and implementation of metabolomics methods, this core will provide state-of-the-art capabilities to facilitate data collection and analysis while ensuring consistency across projects.

项目摘要/摘要 - 核心B 同种异性造血干细胞移植（Allo-HCT）可以治愈慢性感染，通用性疾病或血液癌。但是，其治疗效用受到相关毒性的限制由移植物与宿主疾病（GVHD）产生。 GVHD最突出的病毒是对胃肠道的损害。我们小组的最新结果表明 GVHD受肠道微生物组的性质和代谢特性的影响。此外，我们发现促进微生物群落，促进淀粉的代谢成丁酸酯和重新编程 BMT后，宿主的胆汁酸代谢可以改善，甚至可以防止GVDH。测量和代谢的定量对于解决整个整个相关的实验目标至关重要研究建议。代谢组学核心的目的是支持研究项目基于这些研究以检测和量化小鼠粪便中这些途径的代谢产物，细菌相关临床的患者的人群，人类上皮细胞和纵向粪便样本审判。此外，该核心还将使用稳定提供对宿主和微生物组代谢的全球见解同位素跟踪技术和无靶向代谢范围的分析。通过集中发展，维护和实施代谢组学方法，该核心将提供最新的能够促进数据收集和分析的能力，同时确保跨项目的一致性。