Metabolomics Core

代谢组学核心

基本信息

批准号：
10441576
负责人：
Costas Andreas Lyssiotis
金额：
$ 25.62万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10441576
关键词：
Address Allogenic Amino Acids Bile Acids Bioenergetics Bioinformatics Biological Assay Biostatistics Core Blood specimen Butyrates Carbohydrates Carbon Cells Clinical Trials Custom Data Data Analyses Data Collection Development Disease Enrollment Ensure Epithelial Epithelial Cells Feces Gastrointestinal tract structure Genetic Diseases Genus Hippocampus Glucose Gnotobiotic Graph Hematopoietic Neoplasms Hematopoietic Stem Cell Transplantation Human Incidence Informatics Label Laboratories Maintenance Malignant Neoplasms Mass Spectrum Analysis Measures Metabolic Metabolic Pathway Metabolism Methodology Methods Morbidity - disease rate Mus Nature Nitrogen Nutrient Organism Pathway interactions Patients Pharmaceutical Preparations Plasma Population Production Program Research Project Grants Property Research Project Grants Research Proposals Research Support Resources Safety Severities Starch Statistical Data Interpretation Technology Therapeutic Tissues Toxic effect Tracer Volatile Fatty Acids assay development base bile acid metabolism chronic infection experience gastrointestinal graft vs host disease gut microbiome host microbiome in vivo Model insight large datasets lipidome metabolome metabolomics microbial microbial community microbiome microbiome analysis nutrient metabolism pharmacodynamic biomarker prevent programs stable isotope stool sample uptake

项目摘要

PROJECT SUMMARY/ABSTRACT – CORE B Allogenic hematopoietic stem cell transplant (allo-HCT) can provide a cure for chronic infections, genetic diseases, or cancers of the blood. However, its therapeutic utility is limited by associated toxicities resulting from graft versus host disease (GVHD). Among the most prominent morbidities of GVHD is damage to the gastrointestinal tract. Recent results from our groups have revealed that the severity of GVHD is impacted by the nature and metabolic properties of the gut microbiome. Further, we found that promotion of microbial communities that facilitate the metabolism of starch into butyrate and reprogram bile acid metabolism of the host ameliorate or even prevent GVDH following BMT. The measure and quantitation of metabolism is essential to address the associated experimental aims across the research proposals. The purpose of the Metabolomics core is to support the research projects that build on these studies to detect and quantify metabolites from these pathways in stool from mice, bacterial populations, human epithelial cells, and longitudinal stool samples of patients in the associated clinical trial. Further, this core will also provide global insights into host and microbiome metabolism using stable isotope tracing technologies and untargeted metabolome-wide profiling. By centralizing the development, maintenance, and implementation of metabolomics methods, this core will provide state-of-the-art capabilities to facilitate data collection and analysis while ensuring consistency across projects.

项目概要/摘要 – 核心 B 同种异体造血干细胞移植（allo-HCT）可以治愈慢性感染、遗传性疾病然而，其治疗效用受到相关毒性的限制。由移植物抗宿主病 (GVHD) 引起的 GVHD 最突出的发病率是我们小组的最新结果表明，胃肠道损伤的严重程度。 GVHD 受到肠道微生物组的自然和代谢特性的影响。此外，我们发现。促进促进淀粉代谢为丁酸盐和重新编程的微生物群落 BMT 后宿主的胆汁酸代谢可改善甚至预防 GVDH。代谢的定量对于解决整个领域的相关实验目标至关重要代谢组学核心的目的是支持以下研究项目。以这些研究为基础，检测和量化小鼠、细菌粪便中这些途径的代谢物相关临床中患者的人群、人类上皮细胞和纵向粪便样本此外，该核心还将使用稳定的方法提供对宿主和微生物组代谢的全球见解。通过集中开发，同位素示踪技术和非目标代谢组分析。代谢组学方法的维护和实施，该核心将提供最先进的促进数据收集和分析的能力，同时确保项目之间的一致性。