Intrinsic-contrast super-resolution imaging of DNA at 2-nm resolution

2 纳米分辨率的 DNA 本质对比度超分辨率成像

基本信息

批准号：
10266060
负责人：
Hao F Zhang
金额：
$ 31.6万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-20 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10266060
关键词：
Acids Address Amino Acids Architecture Biological Models Biomedical Research Biopolymers Bypass Cell physiology Cells Cellular Structures Chemistry Chromatin Complex DNA Data Detection Disease Exhibits Fiber Fluorescence Genetic Transcription Genome Goals Image Imaging technology Individual Label Lead Length Methods Microscopy Microtubules Molecular Molecular Profiling Molecular Structure Nanoscopy Normal Cell Nucleotides Optics Photons Play Positioning Attribute Prize Process Proteins Reporting Resolution Role Science Signal Transduction Structure System Technology Three-Dimensional Imaging Time Visible Radiation base biological research biological systems cellular imaging density ds-DNA innovation macromolecular assembly macromolecule molecular recognition molecular scale monomer nano nanometer nanometer resolution nanoscale new technology novel optical imaging optical nanoscopy optical spectra single molecule technology research and development three dimensional structure virtual

项目摘要

PROJECT SUMMARY One of the major recent scientific breakthroughs was the emergence of super-resolution optical nanoscopy. Nanoscale optical imaging currently plays a crucial role in biomedical sciences and has provided invaluable information about cellular structure and function at the macromolecular scale. However, the potential of this technology can be immensely enhanced. In this project a new optical technology, spectroscopic intrinsic-contrast photon-localization optical nanoscopy (SICLON), will be developed that will enable label-free nanoscale imaging of three-dimensional structure and molecular composition of cells with spatial resolution approaching 2 nm. SICLON is based on a newly discovered physical effect: most biopolymers, such as DNA, that until recently had been considered “dark” in the visible regime exhibit stochastic fluorescence switching when illuminated by low- intensity visible light. The technology could have a profound impact on a wide range of biological research as it will become possible to do molecular-resolution, whole-cell, optical imaging without the use of labels.

项目概要最近的重大科学突破之一是超分辨率光学纳米显微镜的出现。纳米级光学成像目前在生物医学科学中发挥着至关重要的作用，并提供了宝贵的信息关于大分子尺度上的细胞结构和功能的信息然而，这种潜力。在这个项目中，一种新的光学技术——光谱本征对比度——可以得到极大的增强。将开发光子定位光学纳米显微镜（SICLON），以实现无标记纳米级成像空间分辨率接近 2 nm 的细胞三维结构和分子组成。 SICLON 基于一种新发现的物理效应：大多数生物聚合物，例如 DNA，直到最近才具有在可见光区域被认为是“暗”的，当受到低光照射时表现出随机荧光切换该技术可能对广泛的生物研究产生深远的影响，因为它将有可能在不使用标签的情况下进行分子分辨率、全细胞、光学成像。