Self-Cleaning Sensor Membranes to Improve Glucose Monitoring In Vivo

自清洁传感器膜可改善体内血糖监测

基本信息

批准号：
7740114
负责人：
Melissa Grunlan
金额：
$ 21.14万
依托单位：
TEXAS ENGINEERING EXPERIMENT STATION
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-17 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7740114
关键词：
Affect Alexa594 Animals Behavior Binding Binding Sites Biological Assay Biosensing Techniques Biosensor Blood Glucose Cell Adhesion Cells Chemical Engineering Chemistry Chronic Disease Collaborations Concanavalin A Dependence Detection Development Devices Dextrans Diabetes Mellitus Diffusion Dimensions Disease Excision Fingers Fluorescence Forearm Glucose Goals Health Histologic Housing Hydrogels Hydrophobicity Immune response Implant In Vitro Individual Intercellular Fluid International Light Measurement Measures Mechanics Membrane Methodology Methods Monitor Non-Invasive Cancer Detection Optics Oxidation-Reduction Patients Phase Transition Photobleaching Polymers Preparation Property Protocols documentation Rattus Reading Recording of previous events Research Retrieval Series Signal Transduction Siloxanes Skin Solid Source Surface Surface Properties Swelling System Technology Temperature Testing Texas Time Tissues Transition Temperature Tube Universities Water Work World Health Organization acrylic acid base capsule chemical synthesis colloidal nanoparticle crosslink density design dextran diabetic diabetic rat experience fluorophore glucose monitor glucose sensor hydrophilicity implantation implanted sensor improved in vivo monomer multidisciplinary nanocomposite nanoparticle novel novel strategies optical sensor prevent public health relevance sensor

项目摘要

DESCRIPTION (provided by applicant): Diabetes is an international health concern with millions of patients worldwide. There is an unmet need to develop a biosensor technology that is a fast and convenient way to monitor blood glucose in diabetics. Thus, the particular goal of this research is the development of a self-cleaning hydrogel sensor membrane which undergoes cyclical, thermally driven removal of adhered cells to improve the efficacy and lifetime of an implanted glucose sensor. Implanted optically based sensors have the potential for continuous detection of an analyte (e.g. glucose). However, sensing is often compromised by the attachment and accumulation of cells from surrounding tissue as part of the host response. As a result, glucose diffusion is diminished and the sensor must be removed and replaced. We will develop novel self-cleaning thermoresponsive nanocomposite hydrogel membranes fabricated in the form of a hollow tube that houses a fluorescent glucose-responsive assay. The sensor will be implanted in the interstitial fluid just beneath the skin. After implantation, the glucose specific sensor will, when illuminated with the optical system developed, provide continuous measurement of fluorescence peaks that are proportional to the glucose concentration. There are three specific aims proposed in this research: (1) iteratively develop thermoresponsive nanocomposite hydrogel sensor membranes and characterize their mechanical properties as well as thermally-modulated swelling/dewelling behavior, surface hydrophilicity/- hydrophobicity, glucose diffusion and cell-release behavior, (2) iteratively evaluate the efficacy of selected thermoresponsive nanocomposite hydrogels sensor membranes to house a glucose-responsive assay, and (3) quantify the in vivo efficacy of the sensors prepared from selected self-cleaning hydrogel membranes containing the glucose-responsive assay, using both normal and diabetic rats. The facilities and individuals represented in this multidisciplinary team from the Biomedical and Chemical Engineering Departments at Texas A&M University are uniquely suited to carry out this research and have expertise in the chemical synthesis of thermoresponsive hydrogels, optical and electrochemical glucose biosensing, as well as biomedical and chemical engineering. The team has a history of collaboration and brings over 18 years of experience in the design and development of optical sensors for glucose and other analytes. PUBLIC HEALTH RELEVANCE: Diabetes mellitus is a debilitating, chronic, disease that affects over 180 million people, according to the World Health Organization, with estimates projecting to 366 million in 2030. The disease requires the patient to monitor glucose levels several times daily. This monitoring is currently non-continuous and performed primarily by using a commercially available finger or forearm stick method blood glucose reading device. Thus, the ultimate goal of this work is the development of an implantable self-cleaning glucose sensor that, once implanted, could be used to monitor glucose continuously with light from a watch-type of device to help patients with diabetes mellitus.

描述（由申请人提供）：糖尿病是一个国际性的健康问题，全世界有数百万患者。开发一种生物传感器技术是一种快速、方便的监测糖尿病患者血糖的方法，这一需求尚未得到满足。因此，这项研究的具体目标是开发一种自清洁水凝胶传感器膜，该膜可以循环地、热驱动地去除粘附的细胞，以提高植入的葡萄糖传感器的功效和寿命。植入式光学传感器具有连续检测分析物（例如葡萄糖）的潜力。然而，作为宿主反应的一部分，传感通常会受到周围组织细胞的附着和积累的影响。结果，葡萄糖扩散减少，必须拆除并更换传感器。我们将开发新型自清洁热响应纳米复合水凝胶膜，该膜以空心管的形式制造，可容纳荧光葡萄糖响应测定。传感器将被植入皮肤正下方的组织液中。植入后，葡萄糖特异性传感器在用所开发的光学系统照射时，将提供与葡萄糖浓度成比例的荧光峰的连续测量。本研究提出了三个具体目标：（1）迭代开发热响应纳米复合水凝胶传感器膜，并表征其机械性能以及热调节膨胀/消溶胀行为、表面亲水性/疏水性、葡萄糖扩散和细胞释放行为， (2) 迭代评估所选热响应纳米复合水凝胶传感器膜用于容纳葡萄糖响应测定的功效，以及 (3) 量化使用正常和糖尿病大鼠，由选定的含有葡萄糖响应测定的自清洁水凝胶膜制备的传感器的体内功效。来自德克萨斯农工大学生物医学和化学工程系的多学科团队中的设施和个人非常适合开展这项研究，并且在热响应水凝胶的化学合成、光学和电化学葡萄糖生物传感以及生物医学和化学合成方面拥有专业知识。化工。该团队有着悠久的合作历史，在葡萄糖和其他分析物光学传感器的设计和开发方面拥有超过 18 年的经验。公共卫生相关性：据世界卫生组织称，糖尿病是一种使人衰弱的慢性疾病，影响超过 1.8 亿人，预计到 2030 年这一数字将达到 3.66 亿。这种疾病要求患者每天多次监测血糖水平。这种监测目前是非连续的，并且主要通过使用市售的手指或前臂棒法血糖读取装置来执行。因此，这项工作的最终目标是开发一种植入式自清洁葡萄糖传感器，一旦植入，就可以通过手表式设备发出的光连续监测血糖，以帮助糖尿病患者。