Multistage LC-MSn for automated glycan isomer assignment of glycopeptides

用于糖肽自动聚糖异构体分配的多级 LC-MSn

• 批准号: 10255170
• 负责人: Miklos Guttman
• 金额: $ 25.66万
• 依托单位: PROTEIN METRICS, LLC
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-16 至 2022-10-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10255170
• 关键词: Address; Adopted; Biological; Biological Response Modifier Therapy; Biology; Catalogs; Cells; Characteristics; Communities; Complex; Computer software; Data; Databases; Detection; Development; Diagnostic; Discrimination; Disease; Dissociation; Future; Glycobiology; Glycopeptides; Glycoproteins; Immune response; Immune system; Institutes; Ions; Isomerism; Laboratories; Letters; Libraries; Link; Mass Fragmentography; Mass Spectrum Analysis; Methods; Milk; Monitor; Monosaccharides; Noise; Oligosaccharides; Onset of illness; Organism; Outcome; Output; Pattern; Peptides; Phase; Play; Polysaccharides; Post-Translational Protein Processing; Protein Glycosylation; Proteins; Proteomics; Protocols documentation; Regulation; Reproducibility; Research Personnel; Role; Scanning; Signal Transduction; Site; Small Business Technology Transfer Research; Software Tools; Structure; Testing; Therapeutic; Time; Work; base; cancer cell; carbohydrate structure; glycoproteomics; glycosylation; immunoregulation; implementation tool; insight; instrumentation; pathogen; prevent; protein aminoacid sequence; prototype; stereochemistry; sugar; tandem mass spectrometry; tool; tumor progression; user-friendly

N- and O-linked glycosylation are some of the most common, abundant, and biologically important posttranslational modifications of proteins, yet they are also some of the most difficult to study. The dominant analytical platform for the study of glycosylation is mass spectrometry, yet this platform on its own is incomplete, because it cannot elucidate glycan topology and linkage stereochemistry. This project will advance the use of mass spectrometry for glycan structure determination, especially for the case of O-linked glycans, by studying the ion propensities of various structural motifs in multi-stage ion trap mass spectrometry, and by building new software for structure inference from such mass spectra.

N-和O-连接糖基化是一些最常见、最丰富且具有生物学意义的糖基化。 蛋白质的重要​​翻译后修饰，但它们也是最困难的一些 学习。糖基化研究的主要分析平台是质谱法， 然而这个平台本身是不完整的，因为它无法阐明聚糖拓扑结构和 连接立体化学。该项目将推进聚糖质谱分析的应用 通过研究离子来确定结构，特别是对于 O-连接聚糖的情况 多级离子阱质谱中各种结构基序的倾向，并通过 构建新的软件，用于根据此类质谱进行结构推断。