Development of a charge-sensitive optical detection system for high-throughput study of small molecules

开发用于小分子高通量研究的电荷敏感光学检测系统

基本信息

批准号：
10255419
负责人：
Nguyen Ly
金额：
$ 84万
依托单位：
BIOSENSING INSTRUMENT, INC.
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-05-18 至 2023-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10255419
关键词：
Address Adoption Affinity Arizona Binding Biochemical Reaction Biological Markers Biomedical Research Biosensing Techniques Buffers Cell physiology Charge Collaborations Computer software Data Analyses Detection Development Diagnosis Disease Drug Industry Drug Screening Drug Targeting Electrons Feedback Fiber Geometry Kinetics Label Measurement Measures Mechanics Membrane Proteins Modeling Molecular Molecular Analysis Molecular Weight Noise Optics Peptide Library Peptides Performance Pharmaceutical Preparations Phase Phosphorylation Phosphorylation Inhibition Post-Translational Protein Processing Procedures Process Proteins Reaction Reader Reading Research Project Grants Sampling Serum Signal Transduction Small Business Innovation Research Grant System Techniques Technology Testing Time United States National Institutes of Health Universities Validation Work anticancer research biomarker discovery commercialization computerized data processing cost cost effective design detection limit detection method detection platform disease diagnosis drug candidate drug development drug discovery electric field high throughput screening innovation instrument interest molecular mass novel strategies optical fiber prototype real world application receptor research and development screening sensor small molecule success usability

项目摘要

TITLE: Development of a charge sensitive optical detection system for high-throughput study of small molecules SUMMARY Measuring the kinetics of small molecule binding to protein receptors and biochemical reactions of proteins, such as post-translational protein modifications, is a basic task in the understanding of diseases, discovering of diagnosis biomarkers, and screening of drugs. Various label-free techniques have been developed, but their sensitivity decreases with the molecular mass, which makes it challenging to detect small molecules and biochemical reactions. A charge sensitive optical detection (CSOD) system is proposed to address this unmet need. The technology is not only sensitive to small molecules, but also compatible with the standard microplate technology, which is particularly suitable for high-throughput applications. The working principle was established and validated with the support of NIH NCI IMAT (Innovative Molecular Analysis Technologies for cancer research) grants (R21 and R33) to the Arizona State University (ASU) team. In this SBIR direct phase II project, Biosensing Instrument Inc. (BI) will work with the ASU team to develop a commercially viable prototype system. We will continue collaborations with potential customers in biomedical research and pharmaceutical industries for testing samples relevant to their interest and validating the performance usability of the developing system. The success of the project will lead to a new high-throughput screening technology for measuring molecular interactions, particularly small molecule interactions with proteins, and post-translational modifications of proteins. These processes are highly important for biomarker discovery, disease diagnosis and drug screening.

标题：开发用于高通量研究的电荷敏感光学检测系统概括测量小分子与蛋白质受体的结合和蛋白质的生化反应的动力学作为翻译后蛋白质修饰，是理解疾病的基本任务，发现诊断生物标志物和药物筛查。已经开发了各种无标签技术，但是他们敏感性随分子质量而降低，这使得检测小分子和生化反应。提出了一个电荷敏感的光学检测（CSOD）系统，以解决此未定的需要。该技术不仅对小分子敏感，而且与标准微型板兼容技术，特别适合高通量应用。建立了工作原则并在NIH NCI IMAT的支持下进行了验证（癌症的创新分子分析技术研究）授予亚利桑那州立大学（ASU）团队的赠款（R21和R33）。在这个SBIR Direct II期项目中， BioSesmensing Instrument Inc.（BI）将与ASU团队合作开发商业上可行的原型系统。我们将继续与生物医学研究和制药行业的潜在客户合作用于测试与它们的兴趣相关的样本并验证开发系统的性能可用性。该项目的成功将导致一种新的高通量筛选技术来测量分子相互作用，尤其是与蛋白质相互作用的小分子相互作用，以及翻译后的修饰蛋白质。这些过程对于生物标志物发现，疾病诊断和药物筛查非常重要。