Development of an integrated smartphone/resistive nanosensor for onsite biomonitoring of exposure to pesticides

开发集成智能手机/电阻纳米传感器，用于农药暴露现场生物监测

基本信息

批准号：
10255622
负责人：
Jun Wang
金额：
$ 25.59万
依托单位：
NANODIAGNOSTIC TECHNOLOGY, LLC
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-06-01 至 2022-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10255622
关键词：
Accident and Emergency department Acute Address Agriculture Area Biological Assay Biological Markers Biological Monitoring Blood Blood specimen Carbon Nanotubes Cellular Phone Cessation of life Chlorpyrifos Cholinesterases Chronic Data Data Analyses Data Reporting Detection Development Devices Diazinon Disasters Dose Drops Environmental Health Environmental and Occupational Exposure Enzyme Inhibition Enzymes Epidemiologist Exposure to Farming environment Fingers Foundations Goals Health Home Human Human Resources In Vitro Industry Insecticides Intervention Laboratories Letters Measurement Measures Methods Monitor Nanotechnology National Institute of Environmental Health Sciences Occupational Occupational Health Organophosphates Performance Pesticides Phase Physicians&apos Offices Poisoning Population Property Reproducibility Research Research Project Grants Risk Sampling Schools Site Small Business Technology Transfer Research Students Surface Technology Testing Time Toxic effect Toxicology Translating Treatment Efficacy United States Wireless Technology Work acute symptom clinical care clinically relevant cloud platform commercialization cost cost effective data management data sharing data sharing networks detection limit farm worker global health glucose monitor innovation interest mHealth meter minimally invasive mobile application nanosensors nanowire novel novel diagnostics organophosphate poisoning pesticide exposure pesticide poisoning point of care point-of-care diagnostics polyaniline portability programs prototype rapid detection response self testing software development statistics tool toxic organophosphate insecticide exposure

项目摘要

Project Summary Organophosphate (OP) pesticides poisoning is a major environmental and occupational health problem worldwide due to its acute or chronic toxicity. Rapid and accurate detection of personal exposure to OP pesticides is critical to both research and clinical care for victims. However, this detection remains highly variable across commercially available assays, e.g., the Ellman method, and such assays may also not adequately detect low but clinically relevant levels of exposure biomarkers. Moreover, these assays are generally performed in the centralized laboratories, and is slow and expensive. These deficits translate into missed opportunities for the timely initiation of the most appropriate treatment and interventions for acutely or chronically poisoned victims. A simple, rapid, non- or minimally invasive biomonitoring tool for onsite sensitive and accurate detection of personal exposure to OP pesticides is highly desirable and fits the interest of NIEHS exposure program. This STTR phase I project is to develop an innovative integrated smartphone/nanosensor platform for onsite rapid and sensitive detection of OP exposure using a tiny drop of finger-stick blood. This nanosensor will take advantage of nanotechnology and wireless technology and utilize an important biomarker, blood cholinesterase, for the detection of personal OP exposure and the resulted tool has the potential to serve as mHealth for environmental and occupational workers. The research will determine the detection limit, sensitivity, dynamic range, and other key performance metrics of the sensing platform using blood samples in vitro dosed with typical pesticides and validate the platform in field using finger-stick blood samples from farmworkers, and demonstrate the feasibility of the platform for sensitive and accurate detection of exposure to OP pesticides. The resulted nanosensor would be a simple, rapid, cost-effective, and minimally-invasive, point-of-care biomonitoring tool for accurate personal exposure assessment and for on-site rapid detection of exposure to OP pesticides. Thus, this novel sensing platform could serve as a personal meter for self-test just like a glucose meter and could also be used in field, physician's office, bedside, and emergency room.

项目概要有机磷（OP）农药中毒是全球主要的环境和职业健康问题由于其急性或慢性毒性。快速准确地检测个人接触 OP 农药的情况是对于受害者的研究和临床护理都至关重要。然而，这种检测在不同地区仍然存在很大差异商业上可用的测定法，例如埃尔曼方法，并且此类测定法也可能无法充分检测低但暴露生物标志物的临床相关水平。此外，这些测定通常在集中实验室，速度慢且昂贵。这些赤字转化为错失的机会对急性或慢性中毒受害者及时启动最适当的治疗和干预措施。一种简单、快速、无创或微创的生物监测工具，用于现场灵敏、准确地检测个人接触 OP 农药是非常可取的，并且符合 NIEHS 接触计划的利益。这 STTR第一阶段项目是开发创新的集成智能手机/纳米传感器平台，用于现场快速和使用一小滴指尖血灵敏地检测 OP 暴露。该纳米传感器将利用纳米技术和无线技术，并利用重要的生物标志物——血液胆碱酯酶，进行检测个人 OP 暴露的结果以及由此产生的工具有可能作为环境和健康领域的移动医疗职业工人。该研究将确定检测限、灵敏度、动态范围等关键使用添加了典型农药的体外血液样本的传感平台的性能指标使用农场工人的指尖采血样本现场验证该平台，并证明其可行性该平台可灵敏、准确地检测 OP 农药的暴露情况。所得纳米传感器将是一种简单、快速、经济高效、微创的即时生物监测工具，可用于准确个人暴露评估和现场快速检测有机磷农药的暴露情况。于是，这部小说传感平台可以作为个人仪表进行自检，就像血糖仪一样，也可以用于现场、医生办公室、床边和急诊室。