NUTRITIONAL AND FUNCTIONAL METHIONNE REQUIREMENTS IN SEPTIC ADOLESCENTS

脓毒症青少年的营养和功能性蛋硫氨酸需求

• 批准号: 7950611
• 负责人: Leticia Castillo
• 金额: $ 0.44万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7950611
• 关键词: Adolescent; Amino Acids; Anabolism; Child; Childhood; Choline; Clinical Research; Computer Retrieval of Information on Scientific Projects Database; Creatine; Critical Illness; Critically ill children; DNA Methylation; Data; Disease Marker; Equilibrium; Functional disorder; Funding; Goals; Gold; Grant; Homocysteine; Homocystine; Institution; Intake; Lymphocyte; Metabolism; Methionine; Newborn Infant; Nutritional; Nutritional Requirements; Outcome; Peripheral; Population; Production; Protein Biosynthesis; Research; Research Personnel; Resources; Severities; Source; Sulfur Amino Acids; Techniques; Time; United States National Institutes of Health; base; improved; oxidation; septic; sound

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This portocol is a continuation of our studies on sulfur amino acid metabolism in critically ill septic children. The immediate objectives of this project are to better define sulfur amino acid requirements in the healthy and in the critically ill septic pediatric population from newborn to adolescent. This is an ambitious goal that can only be approached with one project at a time. In this proposal we are focused exclusively in the healthy and critically ill adolescent population. Amino acid requirements in healthy children are based on extremely limited data. The amino acid requirements for critically ill children in therms of nutritional and functional balance are not known. Methionine, an indispensable sulfur amino acid serves for protein synthesis in addition to DNA methylation and biosynthesis of important compounds such as creatine, choline, etc. However, an excessive methionine administration may contribute to endothelial dysfunction of critical illness through an excessive production of homocysteine. In the present studies we propose to define nutritional requirements of methionine and to begin to explore functional requirements in 196 critically ill septic adolescents and 196 healthy adolescents over a period of 5 years. We propose to modify the "gold" standard techniqure of amino acid requirement determination, the 24h-indicator amino acid oxidation and balance technique, to make it applicable to both, the healthy and critically ill populations. We plan also to correlate methionine intake with severity of disease, markers of endothelial dysfunction and rates of DNA methylation in peripheral lymphocytes. Our long-term aim is to eventually determine the amino acid requirements that will maintain nutritional balance and function in the pediatric population from newborn to adolescents, to improve outcome in the sick population and to establish sound nutritional standards based on quantitative, reliable data in the healthy pediatric population.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该 portocol 是我们对危重脓毒症儿童硫氨基酸代谢研究的延续。 该项目的近期目标是更好地确定从新生儿到青少年的健康和危重脓毒症儿科人群的含硫氨基酸需求。 这是一个雄心勃勃的目标，一次只能通过一个项目来实现。 在这项提案中，我们专门关注健康和危重青少年人群。 健康儿童的氨基酸需求基于极其有限的数据。 危重儿童在营养和功能平衡方面的氨基酸需求尚不清楚。 蛋氨酸是一种不可缺少的含硫氨基酸，除了 DNA 甲基化和重要化合物（如肌酸、胆碱等）的生物合成外，还用于蛋白质合成。然而，过量施用蛋氨酸可能会因同型半胱氨酸的过量产生而导致危重疾病的内皮功能障碍。 在目前的研究中，我们建议定义蛋氨酸的营养需求，并开始探索 5 年内 196 名危重脓毒症青少年和 196 名健康青少年的功能需求。 我们建议修改氨基酸需要量测定的“金”标准技术——24小时指标氨基酸氧化与平衡技术，使其适用于健康人群和危重人群。 我们还计划将蛋氨酸摄入量与疾病严重程度、内皮功能障碍标志物以及外周淋巴细胞 DNA 甲基化率相关联。 我们的长期目标是最终确定从新生儿到青少年的儿科人群维持营养平衡和功能的氨基酸需求，改善患病人群的预后，并根据定量、可靠的数据建立健全的营养标准。健康的儿科人群。