Core 2 - Crystallography

核心 2 - 晶体学

• 批准号: 10245109
• 负责人: JANET L. SMITH
• 金额: $ 36.25万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-17 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10245109
• 关键词: Cell Line; Complex; Crystallization; Crystallography; Development; Electron Microscopy; Genetic Transcription; HIV; In Vitro; Mediating; Methods; Michigan; Mission; Molecular Biology; Molecular Chaperones; National Institute of Diabetes and Digestive and Kidney Diseases; Phase; Plasmids; Preparation; Process; Production; Proteins; Protocols documentation; RNA; RNA chemical synthesis; Research Personnel; Resolution; Structure; Testing; United States National Institutes of Health; Universities; Validation; Virginia; design; design and construction; improved; macromolecule; member; novel; protein complex; repository; research study; vector

Core 2: Crystallography - Summary The CRNA Crystallography Core will collaborate with CRNA investigators to solve crystal structures of RNA-protein complexes, RNAs and proteins relevant to HIV RNA- mediated processes. The Core also provides essential expertise and materials that extend far beyond the crystallography mission and support many macromolecule preparation and analysis aspects of CRNA research. The Core team, led by Smith and Stuckey, offers collaborative expertise in molecular biology tailored for RNAs and RNA- protein complexes, including construct design, expression testing, and development of purification protocols. The resulting vectors, expression plasmids and protocols as well as purified RNAs, RNA-protein complexes and proteins are provided to collaborating investigators in the CRNA, with the Core lab at the University of Michigan acting as a repository for materials and protocols. Two new members of the Crystallography Core team, CRNA investigator Pornillos (U Virginia) and Significant Contributor Zhang (NIDDK, NIH), bring exceptional expertise in the design, production and validation of RNAs and RNA-protein complexes, particularly for crystallization. In CRNA 2.0, the Core will develop novel methods for expression of RNAs, formation of RNA-protein complexes by co-expression, and design of chaperones to aid RNA crystallization. The Core will also establish an in vitro RNA synthesis capability at Michigan. In support of its crystallography mission, the Core will develop cutting-edge methods to aid RNA crystallization, to improve the diffraction quality of crystals, to solve the phase problem for RNA-containing crystals, to improve crystallographic refinement for RNA-containing structures, and to facilitate the combination of crystallography and electron microscopy.

核心2：晶体学 - 摘要 CRNA晶体学核心将与CRNA研究人员合作解决 与HIV RNA相关的RNA-蛋白质复合物，RNA和蛋白质的晶体结构 中介过程。核心还提供了重要的专业知识和材料 远远超出了晶体学任务，并支持许多大分子 CRNA研究的准备和分析方面。由史密斯和 Stuckey，提供针对RNA和RNA-的分子生物学协作专业知识 蛋白质复合物，包括构造设计，表达测试和开发 纯化协议。最终的向量，表达质粒和协议 作为纯化的RNA，提供RNA蛋白质复合物和蛋白质以进行协作 CRNA的调查人员，密歇根大学的核心实验室充当 材料和协议的存储库。晶体学核心的两个新成员 团队，CRNA调查员Pornillos（U Virginia）和重要的贡献者张 （NIDDK，NIH），在设计，生产和验证方面具有出色的专业知识 RNA和RNA-蛋白质复合物，特别是用于结晶。在CRNA 2.0中，核心 将开发用于表达RNA的新方法，RNA蛋白质复合物的形成 通过共表达和设计伴侣的设计以帮助RNA结晶。核心意愿 还要在密歇根州建立体外RNA合成能力。支持它 晶体学任务，核心将开发尖端方法来帮助RNA 结晶，以提高晶体的衍射质量，以解决相位问题 用于含RNA的晶体，以改善含RNA的晶体学改进 结构，并促进晶体学和电子显微镜的组合。