Developing long-term neuro-behavioral recording and real-time processing platforms for naturally behaving animals

为自然行为动物开发长期神经行为记录和实时处理平台

• 批准号: 10245927
• 负责人: Daniel Aharoni
• 金额: $ 140.4万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-10 至 2024-09-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10245927
• 关键词: Address; Aging; Animals; Basic Science; Behavior; Behavioral; Brain; Cells; Cognitive; Communities; Complex; Computer software; Data; Data Set; Databases; Devices; Disease; Educational workshop; Environment; Generations; Hippocampus (Brain); Image; Investigation; Learning; Maps; Microscope; Neurons; Neurophysiology - biologic function; Neurosciences; Neurosciences Research; Process; Resolution; Sleep; Social Behavior; Techniques; Technology; Time; Transgenic Mice; Wireless Technology; animal colony; behavior measurement; computer framework; computerized tools; data sharing; design; environmental enrichment for laboratory animals; experimental study; innovation; insight; light weight; nervous system disorder; neural circuit; neurobehavioral; novel; novel strategies; open source; relating to nervous system; tool; web site; Address; Aging; Animals; Basic Science; Behavior; Behavioral; Brain; Cells; Cognitive; Communities; Complex; Computer software; Data; Data Set; Databases; Devices; Disease; Educational workshop; Environment; Generations; Hippocampus (Brain); Image; Investigation; Learning; Maps; Microscope; Neurons; Neurophysiology - biologic function; Neurosciences; Neurosciences Research; Process; Resolution; Sleep; Social Behavior; Techniques; Technology; Time; Transgenic Mice; Wireless Technology; animal colony; behavior measurement; computer framework; computerized tools; data sharing; design; environmental enrichment for laboratory animals; experimental study; innovation; insight; light weight; nervous system disorder; neural circuit; neurobehavioral; novel; novel strategies; open source; relating to nervous system; tool; web site

A major challenge in neuroscience is to uncover how defined neural circuits in the brain encode, store, modify, and retrieve information. Adding to this challenge is the fact that neural function does not operate in isolation from but rather within living, behaving animals. To tackle this challenge, significant advancement of neural, behavioral, and computational tools is needed along with new experimental approaches to enable the detailed study of neural circuits within the context of complex behavior and naturalistic, ethologically relevant environments. This proposal aims to do exactly this by designing, implementing, and sharing a highly innovative, community driven, neuro-behavioral recording and real- time processing platform capable of uninterrupted, months-long wireless recording of neural and behavioral activity across a colony of animals in large, naturalistic environments. We will develop a new generation of ultra-light weight, fully wireless miniature microscopes (Miniscopes) for neural imaging in truly naturally behaving animals. These wireless Minsicopes will be powered remotely through power-over-distance technology and capable of imaging neural activity with single cell resolution across thousands of neurons. In combination with transgenic mouse lines, these wireless Miniscopes will continuously record neural activity across months as animals live, uninterrupted, in large, enriched environments. An extensive array of behavioral devices will be developed and natively integrated into the platform for animal tracking, parsing complex behaviors, and detecting animal-environment interactions. All tools and techniques built for this project will be actively shared through our open-source website and workshops. Neural and behavioral data will be processed, in real-time, through a novel computational framework (hardware and software) and shared openly through an online database accessible to the neuroscience community. Pilot experiments using this platform will investigate the long-term formation, stability, and generalization of hippocampal cognitive maps within ethologically relevant environments. Once validated, subsequent experiments will incorporate tasks proposed by the neuroscience community encompassing both basic science and investigation of neurological disorders. A single dataset generated with this platform will track a colony of animals’ complex behavior and neural activity through learning, recall, sleep, social behavior, and aging. This novel approach has the potential to fundamentally transform the way neuroscience research is thought about, implemented, and shared and will undoubtedly provide new insight into neural function and disorder.

神经科学中的一个主要挑战是发现大脑，存储，商店中的神经圈的定义如何 修改并检索信息。加上这一挑战是神经功能没有的事实 与行为动物隔离，而是孤立地运作。为了应对这一挑战，重大 需要神经，行为和计算工具的进步以及新的实验性 在复杂行为和 自然主义，伦理上相关的环境。该建议旨在通过设计， 实施并共享高度创新的，社区驱动的，神经行为的记录和现实 时间处理平台，能够对神经和神经的长时间无线记录和 大型自然主义环境中动物群体的行为活动。 我们将开发新一代的超光重量，完全无线的微型显微镜 （Miniscopes）用于真正自然表现的动物的神经成像。这些无线分钟将是 通过电力距离技术远程启动，并能够通过 跨成千上万个神经元的单细胞分辨率。结合转基因小鼠线，这些 随着动物的生存，无线米斯科普斯将继续记录整个几个月的神经活动， 在大，丰富的环境中不间断。各种各样的行为设备将是 开发并本地整合到动物跟踪平台，解析复杂行为以及 检测动物环境相互作用。为该项目构建的所有工具和技术将是积极的 通过我们的开源网站和研讨会共享。神经和行为数据将被处理， 实时通过新颖的计算框架（硬件和软件）并公开共享 通过神经科学社区访问的在线数据库。 使用此平台的试点实验将研究长期形成，稳定性和 在伦理上相关的环境中的海马认知图的概括。一次 经过验证的，随后的实验将纳入神经科学社区提出的任务 涵盖基础科学和神经系统疾病的研究。一个数据集 该平台生成的将通过动物的复杂行为和神经活动来追踪 学习，回忆，睡眠，社会行为和衰老。这种新颖的方法有可能 从根本上改变神经科学研究的思考，实施和共享的方式 毫无疑问，将提供有关神经功能和混乱的新见解。