Flourescence-Activated Cell Sorter

荧光激活细胞分选仪

• 批准号: 7794359
• 负责人: Thomas J Rogers
• 金额: $ 49.9万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-20 至 2011-02-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794359
• 关键词: Atherosclerosis; Basic Science; Biological; Cell Line; Cells; Clinical; Color; Communicable Diseases; Dendritic Cells; Digital Signal Processing; Disease; Endothelial Cells; Flow Cytometry; Funding; Grant; HIV; Hematopoietic stem cells; Human; Infection; Leukocytes; Malignant Neoplasms; Neurons; Osteoblasts; Population; Research; Research Personnel; Research Project Grants; Resistance; Safety; Solid Neoplasm; Sorting - Cell Movement; United States National Institutes of Health; Universities; Viral Vector; Work; cellular transduction; fluorescence activated cell sorter device; improved; instrument; leukemia; medical schools; neoplastic cell; precursor cell; programs

DESCRIPTION (provided by applicant): We are requesting funds that will permit the purchase of a Becton Dickinson Influx Fluorescence Activated Cell Sorter. This instrument will be configured with a biological safety cabinet that will permit the sorting and/or analysis of primary human cell populations, as well as primary cells and cell lines, which have been transduced with various viral vectors. This instrument will also be configured to sort and/or perform multiparmetric analysis using up to 11 colors, and a total of 13 parameters. The NIH funded research projects described in this application all require access to an instrument that has these capabilities. In addition the instrument will further bolster our efforts to expand our NIH-funded research enterprise. This instrument will allow work to be carried out which is part of at least 28 NIH RO1 grants or Program Projects, by investigators in both basic science and clinical departments at Temple University School of Medicine. This top of the line versatile digital signal processing cell sorter will make it possible to carry out flow cytometry with primary human leukocytes, neuronal precursor cells, endothelial cells, osteoblasts, dendritic cells, hematopoietic stem cells, various solid tumor cell populations, leukemias, and a number of transduced cell lines. The NIH-funded projects that are included will make it possible to carry out research directly relevant to improving our understanding of a wide spectrum of diseases including cancer, atherosclerosis, and resistance to a number of infectious diseases, including infection with HIV.

描述（由申请人提供）：我们要求提供资金，以购买Becton Dickinson Influx荧光激活的细胞分系师。该仪器将配置使用生物安全柜，该柜将允许对原代人类细胞群体的分类和/或分析，以及原代细胞和细胞系，这些细胞和细胞系已被各种病毒矢量转导。该仪器还将配置为使用多达11种颜色和总共13个参数进行排序和/或执行多臂分析。 NIH资助的研究项目本应用程序都需要访问具有这些功能的工具。此外，该工具将进一步加强我们为扩大NIH资助的研究企业的努力。该工具将允许进行工作，这是坦普尔大学医学院基础科学和临床部门的研究人员至少是28个NIH RO1赠款或计划项目的一部分。线的顶部多功能数字信号处理细胞分类器将使用原代人白细胞，神经元前体细胞，内皮细胞，成骨细胞，树突状细胞，造血干细胞，各种固体肿瘤细胞，各种固体细胞群，白血病，白血病，以及传输的细胞数量的数量进行流式细胞术。由NIH资助的项目所包含的项目将使与我们对包括癌症，动脉粥样硬化以及对多种传染病（包括HIV感染）的多种疾病的了解直接相关的研究直接相关。