Flourescence-Activated Cell Sorter

荧光激活细胞分选仪

• 批准号: 7794359
• 负责人: Thomas J Rogers
• 金额: $ 49.9万
• 依托单位: TEMPLE UNIV OF THE COMMONWEALTH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-20 至 2011-02-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794359
• 关键词: Atherosclerosis; Basic Science; Biological; Cell Line; Cells; Clinical; Color; Communicable Diseases; Dendritic Cells; Digital Signal Processing; Disease; Endothelial Cells; Flow Cytometry; Funding; Grant; HIV; Hematopoietic stem cells; Human; Infection; Leukocytes; Malignant Neoplasms; Neurons; Osteoblasts; Population; Research; Research Personnel; Research Project Grants; Resistance; Safety; Solid Neoplasm; Sorting - Cell Movement; United States National Institutes of Health; Universities; Viral Vector; Work; cellular transduction; fluorescence activated cell sorter device; improved; instrument; leukemia; medical schools; neoplastic cell; precursor cell; programs

DESCRIPTION (provided by applicant): We are requesting funds that will permit the purchase of a Becton Dickinson Influx Fluorescence Activated Cell Sorter. This instrument will be configured with a biological safety cabinet that will permit the sorting and/or analysis of primary human cell populations, as well as primary cells and cell lines, which have been transduced with various viral vectors. This instrument will also be configured to sort and/or perform multiparmetric analysis using up to 11 colors, and a total of 13 parameters. The NIH funded research projects described in this application all require access to an instrument that has these capabilities. In addition the instrument will further bolster our efforts to expand our NIH-funded research enterprise. This instrument will allow work to be carried out which is part of at least 28 NIH RO1 grants or Program Projects, by investigators in both basic science and clinical departments at Temple University School of Medicine. This top of the line versatile digital signal processing cell sorter will make it possible to carry out flow cytometry with primary human leukocytes, neuronal precursor cells, endothelial cells, osteoblasts, dendritic cells, hematopoietic stem cells, various solid tumor cell populations, leukemias, and a number of transduced cell lines. The NIH-funded projects that are included will make it possible to carry out research directly relevant to improving our understanding of a wide spectrum of diseases including cancer, atherosclerosis, and resistance to a number of infectious diseases, including infection with HIV.

描述（由申请人提供）：我们正在请求资金来购买 Becton Dickinson Influx 荧光激活细胞分选仪。该仪器将配置一个生物安全柜，允许对原代人类细胞群以及已用各种病毒载体转导的原代细胞和细胞系进行分选和/或分析。该仪器还将配置为使用最多 11 种颜色和总共 13 个参数进行排序和/或执行多参数分析。本申请中描述的 NIH 资助的研究项目都需要使用具有这些功能的仪器。此外，该仪器将进一步支持我们扩大 NIH 资助的研究事业。该工具将允许坦普尔大学医学院基础科学和临床部门的研究人员开展至少 28 项 NIH RO1 资助或计划项目的一部分的工作。这种顶级的多功能数字信号处理细胞分选仪将能够对原代人白细胞、神经元前体细胞、内皮细胞、成骨细胞、树突状细胞、造血干细胞、各种实体瘤细胞群、白血病和许多转导的细胞系。其中包含的美国国立卫生研究院资助的项目将使开展与提高我们对多种疾病的理解直接相关的研究成为可能，这些疾病包括癌症、动脉粥样硬化以及对包括艾滋病毒感染在内的多种传染病的抵抗力。