Purchase of a Q-TOF LC/MS System

购买 Q-TOF LC/MS 系统

• 批准号: 7793911
• 负责人: Michael C Fitzgerald
• 金额: $ 36.88万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-09 至 2011-08-08
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793911
• 关键词: 16 year old; 7 year old; Aging; Biological Models; Biopolymers; Chemistry; Computer software; Data; Electrospray Ionization; Environment; Faculty; Funding; Funding Applicant; Housing; Information Systems; Liquid Chromatography; Mass Spectrum Analysis; N.I.H. Research Support; Postdoctoral Fellow; Proteomics; Research Personnel; Research Project Grants; Resource Sharing; Resources; Sampling; System; Technology; Training; Universities; Walking; base; graduate student; instrument; ion source; liquid chromatography mass spectrometry; mass spectrometer; meetings; member; operation; public health relevance

DESCRIPTION (provided by applicant): Funds are requested to purchase an Accurate-Mass Q-TOF LC/MS mass spectrometer system from Agilent Technologies. The major components of the requested mass spectrometer system (Model Number 6520) include: an accurate mass Q-TOF mass spectrometer equipped with a dual spray electrospray ionization (ESI) ion source, an HPLC-Chip cube mass spectrometry (MS) interface, a nanoflow liquid chromatography (LC) system; and the requisite data system and software to acquire, view, and manipulate mass spectral data. The requested instrument, which will be housed and maintained in the Duke Chemistry Department's Instrument Facility, will not only help meet the general mass spectrometry needs of the eight major users outlined in this proposal, but it will also help meet the general mass spectrometry needs of the Facility's entire user base, which includes 20+ faculty members in the Chemistry Department and 100+ graduate students and post-doctoral researchers in the Chemistry Department, as well as number of researchers from other departments at Duke (many of which are involved in NIH-supported research projects). The proposed instrument will facilitate the analysis of a wide variety of sample types, including small organic molecules as well as both synthetic and naturally occurring biopolymers. The Chemistry Department's need to add a Q-TOF mass spectrometer system to its Instrument Facility is three-fold: (1) it will provide replacement capabilities for an aging workhorse, the 16 year old JEOL SX-102 HRMS system, in the Department's Instrument Facility; (2) it will reduce the backlog of samples/users associated with another of the Department's workhorse mass spectrometers, a 7 year old Agilent IonTrap LC-MS system; and (3) it will bring exciting new proteomics capabilities to the Chemistry Department Instrument Facility, which is the only shared resource on campus to offer appropriately trained users direct walk-up access to its instruments. The reliability, robustness, and ease-of-use afforded by the requested instrument with its HPLC-Chip cube MS interface will greatly facilitate operation of the instrument in the Chemistry Department Facility's open access environment. This will make the instrument a unique resource on the Duke campus. PUBLIC HEALTH RELEVANCE: Funds are requested for the Duke University Chemistry Department Instrument Facility to purchase an Accurate-Mass Q-TOF LC/MS mass spectrometer system from Agilent Technologies. The instrument will be uniquely configured to facilitate the characterization of a wide variety of sample types ranging from small organic molecules to large naturally occurring biopolymers. The instrument will be available for direct walk-up use by appropriately trained users of the Duke Chemistry Department's Instrument Facility, many of whom are engaged in NIH-supported research.

描述（由申请人提供）：要求资金从Agilent Technologies购买准确的质量Q-TOF LC/MS质谱仪系统。请求的质谱仪系统的主要组件（型号6520）包括：配备了双喷雾电喷雾电离（ESI）离子源的精确质量Q-TOF质谱仪，HPLC-CHIP-CUBE质谱（MS）界面，一种纳米液体液化（LC）系统；以及需要获取，查看和操纵质谱数据的必要数据系统和软件。该提案中概述的八个主要用户的一般质谱需求不仅将有助于满足该提案中概述的八个主要用户的一般质谱需求，而且还将有助于满足该设施的整个用户基础的一般质谱需要，其中包括化学部门和100多个研究生的研究员，以及其他研究人员的研究，该研究人员还将帮助满足该设施的一般质谱需要，以及其他研究人员以及其他研究人员的研究，该研究人员还将有助于满足杜克化学部仪器设施中所请求的仪器，不仅将有助于满足八个主要用户的一般质谱需求。杜克大学的部门（其中许多涉及NIH支持的研究项目）。所提出的仪器将促进各种样品类型的分析，包括小有机分子以及合成和天然存在的生物聚合物。化学部门需要在其仪器设施中添加Q-TOF质谱仪系统的需求是三个方面：（1）它将为老化的工作马（16岁的JEOL SX-102 HRMS）提供替换功能，该部门的仪器设施中； （2）它将减少与该部门另一个工作马质谱仪相关联的样品/用户的积压，即7岁的Agilent Iontrap LC-MS系统； （3）它将为化学部仪器设施带来令人兴奋的新蛋白质组学功能，这是校园内唯一可以为培训的用户直接访问其仪器的共享资源。请求的仪器带有其HPLC-Chip Cube MS接口提供的可靠性，鲁棒性和易用性，将极大地促进该仪器在化学部门设施的开放访问环境中的运行。这将使乐器成为杜克大学校园的独特资源。 公共卫生相关性：要求资金用于杜克大学化学系工具设施，以从Agilent Technologies购买准确的质量Q-TOF LC/MS质谱仪系统。该仪器将被唯一配置，以促进从小有机分子到大型天然生物聚合物的各种样本类型的表征。该仪器将可供杜克化学部仪器设施的经过适当培训的用户直接使用，其中许多人从事NIH支持的研究。