FoxWare™, An Advanced Data Analysis Package for Hydroxyl Radical Foot-Printing Higher Order Structural Analysis

FoxWare™，一种用于羟基自由基足迹高阶结构分析的高级数据分析包

• 批准号: 10092185
• 负责人: Scot Randy Weinberger
• 金额: $ 49.22万
• 依托单位: GENNEXT TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-01-01 至 2023-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10092185
• 关键词: 3-Dimensional; Address; Adopted; Adoption; Adverse Drug Experience Report; Antibody Binding Sites; Appearance; Area; Automation; Award; Binding; Binding Sites; Biological Products; Biological Response Modifier Therapy; Cessation of life; Chemicals; Client; Comparative Study; Complex; Computer software; Consequentialism; Critiques; Dangerousness; Data; Data Analyses; Development; Docking; Drug Targeting; Environment; Epitopes; Evaluation; Goals; Government; Guidelines; Higher Order Chromatin Structure; Home; Hydroxyl Radical; Immune response; Industry; Label; Laboratories; Lasers; Letters; Ligands; Link; Location; Manuals; Methods; Modeling; Morbidity - disease rate; Morphologic artifacts; Nature; Patients; Peptides; Pharmaceutical Preparations; Pharmacology; Phase; Play; Process; Production; Protein Analysis; Protein Footprinting; Proteins; Proteomics; Reaction; Research; Research Personnel; Resolution; Role; Safety; Sampling; Scientist; Small Business Innovation Research Grant; Structural defect; Structure; Techniques; Technology; Translating; Ultraviolet Rays; Visualization; Visualization software; Work; adverse drug reaction; analytical tool; biomaterial compatibility; combat; computerized data processing; experimental study; improved; infliximab; interest; irritation; new technology; oxidation; processing speed; programs; protein protein interaction; protein structure; prototype; research and development; software development; tool

The GenNext Phase II SBIR submssion entitled “FoxWare™, an Advanced Data Analysis Package for Hydroxyl Radical Foot-printing Higher Order Structural Analysis” is responsive to the ackowledged need for new and improved tools for higher order structural analysis (HOS) of biopharmaceuticals. Biopharmaceuticals are complex, heterogeneous mixtures of 3-dimensional biomolecules, whose safety and efficacy is reliant upon proper HOS. The presence of proteins with improper HOS has been linked to severe adverse drug reactions (ADR), establishing the need for new and improved HOS analytics. An emerging HOS analysis technique is hydroxyl radical foot-printing (HRPF). HRPF involves the irreversible labeling of a protein's exterior by reaction with hydroxyl radicals with subsequent MS analysis to identify the outer portions of the protein. The most widely used method for generating OH radicals employs a quick burst of UV light, and is appropriately called fast photochemical oxidation of proteins (FPOP). Academic laboratories have demonstrated the utility of FPOP for HOS analysis; however adoption in pharma has been minuscule at best. Guided by the critiques of scientists at leading biopharmaceutical companies, we have identified barriers that have limited pharma's adoption of FPOP HRPF. A substantial barrier is the absence of data processing tools to facilitate HRPF analysis. Today there are no commercial solutions for FPOP analysis, despite the demonstrated market need for its HOS analytical power. Today's proteomics data analysis products fail to adequately address specific work-flow, chemical labeling / artifacts, and comparative study requirements of HRPF HOS analysis. Researchers are compelled to use a piece-meal approach, arduously stitching together results from multiple packages and manually manipulating data in home-brew spreadsheets. As such, data processing represents a significant bottleneck that greatly complicates and delays progress in HRPF HOS studies. Our FoxWare™ software addresses data processing demands of HRPF HOS analysis. Our proposal vastly improves HRPF data analysis by replacing laborious and agonizing present-day practices with an integrated, seamless solution to interpret and amplify the value of HRPF HOS studies. Upon completion of our envisioned program, FoxWare will make an indelible mark upon HOS analysis and impart a transformative shift in analytics and HRPF throughput, addressing the market demands of the biopharmaceutical industry.

GenNext II期SBIR子梅塞sion标题为“ Foxware™，高级数据 羟基自由基脚印高阶结构分析的分析包” 响应对高阶新工具的敏锐的需求 生物制药的结构分析（HOS）。生物制药很复杂， 三维生物分子的异质混合物，其安全性和效率为 依靠适当的hos。 HOS不当的蛋白质的存在已连接 严重不良药物反应（ADR），确定了对新的和改进的需求 HOS分析。 新兴的HOS分析技术是羟基自由基（HRPF）。 HRPF涉及与羟基反应的蛋白质外部的不可逆标记 随后进行MS分析的自由基鉴定蛋白质的外部。这 最广泛使用的方法来生成OH激进分子员工快速爆发紫外线， 并适当称为蛋白质（FPOP）的快速光化学氧化。学术的 实验室已经证明了FPOP用于HOS分析的实用性。但是采用 在制药中，充其量只是很小。受到领导的科学家的批评的指导 生物制药公司，我们已经确定了限制Pharma的障碍 采用FPOP HRPF。一个实质性的障碍是缺乏数据处理 促进HRPF分析的工具。今天没有用于FPOP的商业解决方案 分析，策略证明了对其HOS分析能力的市场需求。 当今的蛋白质组学数据分析产品无法充分解决特定的问题 HRPF的工作流，化学标签 /工件和比较研究要求 HOS分析。研究人员被迫使用碎片方法，艰难地 将多个软件包的结果拼接在一起，并手动操纵数据 自家式电子表格。因此，数据处理代表着重要的瓶颈 这极大地复杂化并延迟了HRPF HOS研究。我们的Foxware™ 软件解决了HRPF HOS分析的数据处理需求。我们的建议 通过替换实验室和痛苦，可以极大地改善HRPF数据分析 具有集成，无缝解决方案的实践，以解释和扩大 HRPF HOS研究。我们设想的计划完成后，Foxware将成为 在HOS分析中不可避免的标记，并赋予分析和分析的变革性转变 HRPF吞吐量，满足生物制药行业的市场需求。