Renal Tissue Derived From Amniotic Fluid Stem Cells

羊水干细胞衍生的肾组织

基本信息

批准号：
7676650
负责人：
Roger Edward De Filippo
金额：
$ 12.09万
依托单位：
CHILDREN'S HOSPITAL OF LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-09-30 至 2011-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7676650
关键词：
Adult American Amniotic Fluid Anatomy Animal Model Biology Biomedical Engineering Cell Line Cells Coculture Techniques Data Development Developmental Biology ES Cell Line Embryo Embryonic Development Embryonic Structures End stage renal failure Epidemic Goals Growth Factor Histologic In Vitro Individual Kidney Kidney Diseases Kidney Failure Kidney Transplantation Laboratories Lead Mesenchyme Metanephric Diverticulum Metanephric structure Mus Natural regeneration Nude Mice Organ Organ Donor Pathway interactions Patients Population Process Property Renal Tissue Research Personnel Retrieval Role Signaling Protein Source Staging Stem cells System Technology Testing Therapeutic Time Tissue Engineering Tissues Transplantation United States amniotic fluid derived stem cell embryo tissue in vivo insight interest kidney cell mutant mouse model novel novel strategies programs scale up tissue regeneration

项目摘要

DESCRIPTION (provided by applicant): A growing shortage of donor organs has heightened interest in developing novel strategies for tissue regeneration, including the possible role of stem cells, as an alternative to heterologous organ replacement in patients with renal failure. We have discovered that a unique population of pluripotential cells exist in amniotic fluid that have similar totipotent properties as more traditional lines of embryonic stem cells but avoid embryo destruction during retrieval, which is desirable. We would like to apply these amniotic fluid derived stem cells for the regeneration of kidney tissue in the laboratory. Tissue Engineering and Developmental Biology have uniquely contributed compelling but preliminary data towards a common goal of bioengineering kidneys. Therefore, the broad objective of this proposal is to determine if amniotic fluid derived stem cells are capable of forming kidney tissue in a novel co-culture system that employs, for the first time, technologies from both Tissue Engineering and Developemental Biology. This project also seeks to optimize the scalability of these bioengineered kidneys so that they can be applied in vivo.

描述（由申请人提供）：供体器官的日益短缺引起了人们对开发新的组织再生策略的兴趣，包括干细胞的可能作用，作为肾衰竭患者异源器官替代的替代方案。我们发现羊水中存在独特的多能细胞群，它们具有与更传统的胚胎干细胞系相似的全能特性，但避免了胚胎在检索过程中被破坏，这是所希望的。我们希望在实验室中应用这些羊水干细胞来再生肾组织。组织工程和发育生物学为实现肾脏生物工程的共同目标提供了令人信服但初步的数据。因此，该提案的主要目标是确定羊水来源的干细胞是否能够在新型共培养系统中形成肾组织，该系统首次采用组织工程和发育生物学的技术。该项目还寻求优化这些生物工程肾脏的可扩展性，以便它们可以在体内应用。