Skin-associated B cells in allergy

过敏中的皮肤相关 B 细胞

基本信息

批准号：
10088409
负责人：
Loren D Erickson
金额：
$ 20.19万
依托单位：
UNIVERSITY OF VIRGINIA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-02-01 至 2022-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10088409
关键词：
Acute Affect Allergens Allergic Allergic Disease Allergic Reaction Animals Antibodies Antibody Formation Antigens Autoimmunity B-Lymphocyte Subsets B-Lymphocytes Blood Group Antigens Blood specimen CCR6 gene Cell Lineage Cells Characteristics Chimera organism Chronic Chronic Disease Consumption Control Animal Cutaneous Data Development Disease Eating Exhibits Exposure to Food Food Hypersensitivity Functional disorder Galactose Gene Expression Genes Genetic Genetic Transcription Health Heterogeneity Human Hypersensitivity IgE Immune Immunoglobulin Class Switching Immunoglobulin D Immunoglobulin M In Vitro Individual Infection Inflammation Kinetics Lead Life Link Maintenance Meat Mediating Memory Memory B-Lymphocyte Mus Oral Ingestion Outcome Study Pathogenesis Pathology Pathway interactions Patients Phenotype Plasma Plasma Cells Play Population Prevalence Production Public Health Reaction Reporter Resolution Risk Role Site Skin Societies Structure of germinal center of lymph node System Testing Thinking Ticks Tissues United States alpha-gal syndrome base beef chronic inflammatory disease design dietary dimensional analysis effective therapy gastrointestinal high dimensionality high risk insight mouse model novel prevent respiratory skin barrier targeted treatment tick bite tool transcriptome treatment strategy

项目摘要

Project Abstract A number of human chronic inflammatory diseases caused by infection, autoimmunity and allergy are strongly linked to B cells that infiltrate the skin, yet the precise role of skin-associated B cells in disease pathogenesis remains unclear. In particular, the underlying mechanisms by which skin exposure to allergens leads to the development and maintenance of IgE production has not been investigated, and a direct contribution by disease-specific B cells to inflammation and allergic reactions in tissue sites such as skin remains to be fully explored. In the studies proposed here we will characterize an expanded population of B cells found in skin of patients with food allergy to red meat and in a novel mouse model of meat allergy our lab recently developed. We will explore the kinetics and phenotype of CCR6hi B cells that accumulate in inflamed skin, evaluate the specific contribution of CCR6hi B cells to disease as well as their function as allergen-specific IgE-producing cells. These efforts will provide a broad assessment of the features and potential functions of this highly expanded B cell population in human allergy.

项目摘要由感染，自身免疫和过敏引起的许多人类慢性炎症性疾病强烈与浸润皮肤的B细胞有关，但皮肤相关B细胞在疾病发病机理中的精确作用仍然不清楚。特别是，皮肤暴露于过敏原的基本机制导致尚未调查IGE生产的开发和维护，并直接贡献疾病特异性的B细胞对诸如皮肤等组织部位的炎症和过敏反应尚待完全探索。在此处提出的研究中，我们将表征在皮肤中发现的B细胞群食物过敏对红肉的患者以及在我们实验室最近开发的新型肉类过敏的新型肉类过敏模型中。我们将探索累积在发炎皮肤中的CCR6HI B细胞的动力学和表型，评估 CCR6HI B细胞对疾病的特定贡献及其作为过敏原特异性IgE产生的功能细胞。这些努力将对高度的特征和潜在功能进行广泛的评估人类过敏的B细胞种群扩大。