Recruitment, Data Collection & Sample Management Core

招聘、数据收集

• 批准号: 10088088
• 负责人: Anne L. Wright
• 金额: $ 137.84万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-10 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10088088
• 关键词: Arizona; Asthma; Birth; Blood; Blood specimen; Child; Childhood; Childhood Asthma; Chronic Disease; Clinic; Clinical; Cohort Studies; Collaborations; Collection; Communities; Data; Data Collection; Databases; Development; Diet; Disease; Enrollment; Environment; Environmental Exposure; Environmental Risk Factor; Equipment and supply inventories; Exposure to; Feces; Genes; Goals; Health; High Prevalence; Home environment; Human Resources; Immune; Immune response; Immunologics; Infant; Infrastructure; Laboratories; Life; Low Prevalence; Mexican; Mexican Americans; Mexico; Microbe; Mothers; Natural experiment; Outcome; Pattern; Play; Population; Pregnancy Trimesters; Pregnant Women; Prevalence; Quality Control; Questionnaires; Research; Resources; Risk; Role; Sampling; Smoking; Specimen; Study Subject; Swab; Third Pregnancy Trimester; Time; Vagina; Woman; asthma model; data resource; experience; fetal; follow-up; gut microbes; gut microbiome; gut microbiota; human microbiota; microbial; microbial community; microbiome; microbiome research; microbiota; mouse model; pet animal; prenatal; prenatal exposure; programs; recruit; sample collection; stool sample

BEAMS ABSTRACT: Core B The prevalence of childhood asthma varies dramatically between populations living in different environments, suggesting that pre-natal and early life environmental exposures play a role in establishing risk for this common disease through influencing patterns of gut microbiota and immune development. The Binational Early Asthma and Microbiome Study (BEAMS) Program Project takes advantage of a natural experiment in our own backyard to elucidate the microbial origins of asthma protection. It will initiate a birth cohort study in two populations with shared ancestry but dramatically different environments, which we have shown to have high and low prevalence of asthma: Mexican American children born in Tucson, Arizona and children born in Nogales, Mexico, respectively. The Recruitment, Data Collection and Sample Management Core (Core B), is critical to this effort as it will direct, coordinate and complete all data collection on subjects enrolled in BEAMS. Core B will serve all three projects proposed in this application, collecting blood and data for Project 1 and all the microbiota and environmental samples used in Projects 2 and 3. Specific aims of Core B are to: 1) Enroll 250 Mexican American women in Tucson, Arizona and 250 Mexican women in Nogales, Mexico in the third trimester of pregnancy, obtain samples for immunologic and microbiome assessments, obtain environmental samples, and administer questionnaires for identification of prenatal exposures and potential covariates; 2) Follow the children of these women from birth through the first 24 months of life, sampling stool and blood repeatedly, obtaining environmental samples and administering questionnaires on exposures and intercurrent illnesses that may influence asthma risk or the development of the gut microbiome and clinical outcomes; and 3) Provide the resources for collection of all data on BEAMS subjects and manage all samples and questionnaires obtained from collection in the field through sample processing in the labs, including inventory control, data entry and quality control, and distribution to the appropriate laboratories. Core B will provide the resources for collection of all data on BEAMS subjects, including personnel experienced in subject recruitment, home environmental assessments, blood sampling and questionnaire administration, a laboratory for inventorying and processing of environmental samples, and an infrastructure for specimen transport across the border. Core B will leverage enduring relationships with two comprehensive health centers that will serve as our clinical partners for recruitment and follow-up of mother/infant dyads. The Core B team includes national leaders in the conduct of highly successful birth cohort studies and border health who bring long-term relationships with the communities that will participate in the study. By implementing a coordinated approach to sample and data collection, Core B will assure that comprehensive information is obtained on pregnant women and their children from birth through 24 months in this unique border setting, thereby assuring the Program will achieve its overarching goals.

梁摘要：核心 B 生活在不同环境的人群之间，儿童哮喘的患病率差异很大， 表明产前和生命早期的环境暴露在确定这种常见风险方面发挥着作用 通过影响肠道微生物群和免疫发育的模式来治疗疾病。两国早期哮喘 和微生物组研究 (BEAMS) 项目利用我们自家后院的自然实验 阐明哮喘保护的微生物起源。它将在两个人群中启动一项出生队列研究 共同的祖先，但环境却截然不同，我们已经证明它们的患病率有高有低 哮喘：出生在亚利桑那州图森市的墨西哥裔美国儿童和出生在墨西哥诺加利斯的儿童， 分别。招聘、数据收集和样本管理核心（核心 B）对此至关重要 它将指导、协调和完成 BEAMS 注册受试者的所有数据收集。核 B 将为本申请中提出的所有三个项目提供服务，为项目 1 和所有项目收集血液和数据。 项目 2 和 3 中使用的微生物群和环境样本。核心 B 的具体目标是： 1) 招募 250 名 亚利桑那州图森市的墨西哥裔美国妇女和墨西哥诺加利斯市的 250 名墨西哥妇女在妊娠晚期 怀孕，获取样本进行免疫和微生物组评估，获取环境样本， 并进行问卷调查以识别产前暴露和潜在协变量； 2）遵循 这些妇女的孩子从出生到生命的前 24 个月，反复采样粪便和血液， 获取环境样本并进行有关暴露和并发疾病的调查问卷 可能影响哮喘风险或肠道微生物群的发育和临床结果； 3) 提供 用于收集 BEAMS 受试者所有数据并管理获得的所有样本和问卷的资源 从现场采集到实验室样品处理，包括库存控制、数据输入和 质量控制，并分发给适当的实验室。 Core B将提供资源收集 BEAMS 受试者的所有数据，包括在受试者招募、家庭环境方面经验丰富的人员 评估、血液采样和问卷管理、库存和处理的实验室 环境样本，以及跨境样本运输的基础设施。核心B将利用 与两个综合健康中心建立持久的关系，这两个中心将作为我们的临床合作伙伴 母婴二人组的招募和随访。核心 B 团队包括负责开展以下工作的国家领导人 非常成功的出生队列研究和边境卫生，与社区建立了长期关系 将参与该研究。通过实施协调一致的样本和数据收集方法，Core B 将确保获得孕妇及其孩子出生后的全面信息 在这个独特的边境环境中度过 24 个月，从而确保该计划将实现其总体目标。