Michigan MoTrPAC Chemical Analysis Site (MiCAS)

密歇根 MoTrPAC 化学分析站点 (MiCAS)

基本信息

批准号：
10079482
负责人：
CHARLES F BURANT
金额：
$ 177.22万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-12-08 至 2022-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10079482
关键词：
Adherence Adipocytes Adipose tissue Adopted Analytical Chemistry Animals Bioinformatics Biological Biological Assay Biology Biometry Cells Charge Chemicals Clinical Clinical Research Collaborations Communities Computers Consultations Country Data Data Analyses Data Collection Data Element Elements Ensure Exercise Funding Goals Grant Guidelines Health Health Benefit Human Infrastructure Institutes Knowledge Laboratories Leadership Management Information Systems Manuscripts Mass Fragmentography Measures Metabolic Metadata Methodology Methods Michigan Molecular Molecular Analysis Obesity Phase Phenotype Physical activity Plasma Pre-Clinical Model Preparation Procedures Process Production Proteins Protocols documentation Publications Publishing Quality Control Recommendation Reproducibility Research Research Design Research Personnel Risk Rodent Role Sampling Sampling Studies Science Scientist Secure Site Skeletal Muscle Specimen State Interests Statistical Data Interpretation Structure System Technical Expertise Techniques Technology Testing Time Tissue Banks Tissues Training Transducers United States National Institutes of Health Universities Work base biomarker development biomarker validation clinical phenotype clinical research site computerized data processing data cleaning data exchange data integration data management data portal data quality data reduction data repository data submission data visualization design exercise training experience human subject human tissue improved insight interest large scale data lipidomics member metabolomics molecular phenotype molecular site multiple omics new technology novel operation phenotypic data pre-clinical preclinical study preservation prevent programs quality assurance recruit repository response sample collection secondary analysis small molecule tool usability

项目摘要

The University of Michigan’s Molecular Transducers of Physical Activity (MoTrPAC) Chemical Analysis Site (MiCAS) is designed to provide large-scale, high-quality metabolomics analysis in collaboration with the MoTrPAC consortium. The effort will be carried out using state-of-the-art metabolomics infrastructure by a team of chemists, spectroscopists, computer scientists and statisticians in the Michigan Metabolomics and Obesity Center (MMOC), the umbrella administrative structure for several NIH-funded initiatives. MiCAS will be led by investigators with extensive knowledge of exercise science and metabolomics and with experience collaborating with scientists from around the country, obtained in part through leadership roles in NIH-funded projects. The analytical platforms within MiCAS provide the ability to perform a wide variety of targeted, untargeted and flux analysis of metabolites in diverse systems from cells to humans. Core investigators have developed and published novel techniques and technologies to perform metabolomics profiling and to integrate these approaches with clinical studies and other molecular phenotyping methods to improve understanding of the biology of exercise and its relationship to metabolic health. MiCAS will contain 3 cores which will expand on the existing administrative and technical expertise. The Administrative Core will oversee the operations of MiCAS, including efficient utilization of the MMOC infrastructure, manage the Laboratory Information Management System (LIMS) and ensure adherence to Core and MoTrPAc-mandated SOP. The Administrative Core will be in charge of the scientific and administrative interactions with the NIH and MoTrPAc consortium, especially the other Chemical Analysis Sites. The Analysis Core will work across the MoTrPAc consortium to carry out analysis of the wide variety of samples that will be generated in the projects, to develop new technologies as needed and to provide assistance and input regarding the analytical workflows adopted by the consortium. The Bioinformatics Core will provide data management including data cleaning, data reduction, quality control for accuracy and reproducibility, missing feature identification and integration, normalization and interpolation under agreed-upon guidelines. The Core will provide initial statistical analysis of the data and help provide MoTrPAc and associated investigators with expertise and tools for integration of the data with data derived from the other MoTrPAc sites, including data generated across multiple ‘omics platforms. Finally, the Bioinformatics Core will ensure timely transfer of accurate and verified data to designated repositories.

密歇根大学体力活动分子传感器 (MoTrPAC) 化学分析站点 (MiCAS) 旨在与以下机构合作提供大规模、高质量的代谢组学分析 MoTrPAC 联盟将使用最先进的代谢组学基础设施来开展这项工作。密歇根代谢组学的化学家、光谱学家、计算机科学家和统计学家团队肥胖中心 (MMOC) 是 NIH 资助的多项 MiCAS 项目的总括管理机构。由具有运动科学和代谢组学广泛知识和经验的研究人员领导与来自全国各地的科学家合作，部分是通过在 NIH 资助的领导角色中获得的 MiCAS 内的分析平台能够执行各种有针对性的项目。核心研究人员对从细胞到人类的不同系统中的代谢物进行了非靶向和通量分析。开发并发布了进行代谢组学分析和整合的新技术和技术这些方法与临床研究和其他分子表型分析方法相结合，以提高对运动的生物学及其与代谢健康的关系。 MiCAS 将包含 3 个核心，将扩展现有的管理和技术专业知识。行政核心将监督 MiCAS 的运营，包括 MMOC 的有效利用基础设施，管理实验室信息管理系统 (LIMS) 并确保遵守核心 MoTrPAc 授权的 SOP 行政核心将负责科学和行政工作。与 NIH 和 MoTrPAc 联盟的互动，尤其是其他化学分析站点。 Analysis Core 将与 MoTrPAc 联盟合作，对各种样品进行分析将在项目中产生，根据需要开发新技术并提供援助和生物信息学核心将提供有关该联盟采用的分析工作流程的意见。数据管理，包括数据清理、数据缩减、准确性和再现性的质量控制，根据商定的准则缺少特征识别和集成、标准化和插值。核心将提供数据的初步统计分析，并帮助提供 MoTrPAc 和相关的拥有专业知识和工具的研究人员可以将数据与其他 MoTrPAc 的数据进行整合最后，生物信息学核心将确保。及时将准确且经过验证的数据传输到指定存储库。