Metabolomics signatures underlying diet, lifestyle and gut microbiota for diabetes

糖尿病饮食、生活方式和肠道微生物群的代谢组学特征

• 批准号: 10115710
• 负责人: Qibin Qi
• 金额: $ 55.68万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-03-26 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10115710
• 关键词: Accelerometer; Age; Amino Acids; Animals; Behavior; Bile Acids; Biological; Biological Markers; Blood; Characteristics; Clinic Visits; Clinical; Communities; Consumption; Data; Development; Diabetes Mellitus; Diabetes prevention; Diet; Diet Records; Dietary Factors; Energy Intake; Fasting; Follow-Up Studies; Food; Functional disorder; Future; Genetic; Genomics; Glycosylated hemoglobin A; Goals; Habits; Health Professional; Heterogeneity; High Prevalence; Hispanic Community Health Study; Hispanic Community Health Study/Study of Latinos; Hispanics; Hyperglycemia; Individual; Insulin; Insulin Resistance; Intervention; Laboratories; Latino; Life Style; Link; Lipids; Longterm Follow-up; Measures; Meat; Mediating; Metabolic Pathway; Metabolism; Metagenomics; Microbe; Minority Groups; Molecular; Nitrogen; Non-Insulin-Dependent Diabetes Mellitus; Not Hispanic or Latino; Nurses' Health Study; OGTT; Participant; Pathway Analysis; Pathway interactions; Physical activity; Plasma; Population; Prevention; Process; Prospective cohort study; Proteins; Public Health; Reporting; Research Design; Role; Sampling; Standardization; Study of Latinos; Subgroup; Work; acylcarnitine; base; biological specimen archives; case control; cohort; diabetes risk; dietary; doubly-labeled water; experimental study; fasting glucose; follow-up; good diet; gut microbiome; gut microbiota; high risk; insight; lifestyle factors; metabolomics; microbial; microbiome research; microbiota profiles; migration; network models; novel; protein intake; public health relevance; recruit; secondary analysis; small molecule; social culture; tool; trimethyloxamine; urinary; Accelerometer; Age; Amino Acids; Animals; Behavior; Bile Acids; Biological; Biological Markers; Blood; Characteristics; Clinic Visits; Clinical; Communities; Consumption; Data; Development; Diabetes Mellitus; Diabetes prevention; Diet; Diet Records; Dietary Factors; Energy Intake; Fasting; Follow-Up Studies; Food; Functional disorder; Future; Genetic; Genomics; Glycosylated hemoglobin A; Goals; Habits; Health Professional; Heterogeneity; High Prevalence; Hispanic Community Health Study; Hispanic Community Health Study/Study of Latinos; Hispanics; Hyperglycemia; Individual; Insulin; Insulin Resistance; Intervention; Laboratories; Latino; Life Style; Link; Lipids; Longterm Follow-up; Measures; Meat; Mediating; Metabolic Pathway; Metabolism; Metagenomics; Microbe; Minority Groups; Molecular; Nitrogen; Non-Insulin-Dependent Diabetes Mellitus; Not Hispanic or Latino; Nurses' Health Study; OGTT; Participant; Pathway Analysis; Pathway interactions; Physical activity; Plasma; Population; Prevention; Process; Prospective cohort study; Proteins; Public Health; Reporting; Research Design; Role; Sampling; Standardization; Study of Latinos; Subgroup; Work; acylcarnitine; base; biological specimen archives; case control; cohort; diabetes risk; dietary; doubly-labeled water; experimental study; fasting glucose; follow-up; good diet; gut microbiome; gut microbiota; high risk; insight; lifestyle factors; metabolomics; microbial; microbiome research; microbiota profiles; migration; network models; novel; protein intake; public health relevance; recruit; secondary analysis; small molecule; social culture; tool; trimethyloxamine; urinary

Summary/Abstract Metabolomics profiling of blood can detect subtle changes in metabolism that may presage the later development of type 2 diabetes (T2D). This can both reveal early steps in the pathway to T2D, and also provide a novel tool to identify high risk individuals. Hispanics/Latinos, the largest and fastest growing US minority population, have a 66% higher prevalence of diabetes compared to non-Hispanic whites. US Hispanics/Latinos are diverse in biological and sociocultural characteristics, and thus may have distinct diet, lifestyle and gut microbiota patterns which likely influence metabolites implicated in T2D. The Main Goal of this project is to identify metabolomics signatures for T2D risk and examine how diet, lifestyle and gut microbiota influence metabolomics profiles associated with T2D. In particular, we propose to perform high-throughput metabolomics profiling (>400 known and hundreds of unknown metabolites) of plasma from 1000 pairs of T2D incident cases and matched controls in the Hispanic Community Health Study of Latinos (SOL) to identify novel metabolomics signatures associated with incident T2D in US Hispanics/Latinos. In the SOL, we will leverage existing extensive data on laboratory measures (e.g. fasting glucose and insulin, HbA1c, and 2-hr post oral glucose tolerance test) and objectively measured behaviors (e.g., accelerometry, biomarker- measured dietary factors) relevant to T2D. Utilizing gut microbiome data from an ongoing gut microbiome project in the SOL, we will characterize gut microbial composition and functional features associated with plasma metabolomics profiles implicated in T2D and examine the role of diet in modulating gut microbiota- related metabolites. Metabolomics profiles implicated in T2D will be further examined in non-Hispanic cohorts by including 1,500 pairs of T2D incident cases and matched controls from the Nurses' Health Study (NHS) and the Health Professionals Follow-up Study (HPFS), two ongoing prospective cohort studies with the identical metabolomics profiling platform, gut microbiome data and objectively measured diet and lifestyle factors. The inclusion of both Hispanic and non-Hispanic cohorts may offer unique insights into the role of diet/lifestyle, gut microbiota, and metabolic pathways in the development of T2D. The findings may have great potential to identify novel targets for T2D prevention and intervention.

摘要/摘要 血液的代谢组学分析可以检测出可能预示以后的代谢的细微变化 2型糖尿病（T2D）的发展。这既可以揭示通往T2D的途径的早期步骤，也可以 提供一种新颖的工具来识别高风险个人。西班牙裔/拉丁美洲人，我们成长最大，增长最快的我们 与非西班牙裔白人相比，少数族裔人口的糖尿病患病率高66％。我们 西班牙裔/拉丁美洲人在生物学和社会文化特征上都是多样的，因此可能具有独特的饮食， 可能影响与T2D有关的代谢产物的生活方式和肠道菌群模式。主要目标 项目是为了确定T2D风险的代谢组学特征，并检查饮食，生活方式和肠道微生物群如何 影响与T2D相关的代谢组学谱。特别是，我们建议执行高通量 从1000对T2D的血浆的代谢组学分析（> 400个已知和数百个未知代谢产物） 在拉丁美洲人（SOL）的西班牙裔社区健康研究中的事件案例和匹配的控制案件以识别 与美国西班牙裔/拉丁美洲人的事件T2D相关的新型代谢组学特征。在溶胶中，我们将 利用实验室措施的现有广泛数据（例如禁食葡萄糖和胰岛素，HBA1C和2小时 口服葡萄糖耐量测试）和客观测量的行为（例如，加速度计，生物标志物 - 测量的饮食因素）与T2D有关。利用正在进行的肠道微生物组中的肠道微生物组数据 SOL中的项目，我们将表征与肠道微生物组成和功能特征 与T2D有关的血浆代谢组学特征，并检查饮食在调节肠道微生物群中的作用 相关代谢物。与T2D有关的代谢组学剖面将在非西班牙裔人群中进一步研究 通过包括1,500对T2D事件案例和护士健康研究（NHS）和 卫生专业人员后续研究（HPFS），两项正在进行的前瞻性队列研究，相同 代谢组学分析平台，肠道微生物组数据以及客观地测量饮食和生活方式因素。这 包括西班牙裔和非西班牙裔人群可能会为饮食/生活方式的作用提供独特的见解 T2D发展中的微生物群和代谢途径。这些发现可能具有很大的潜力 确定预防和干预T2D的新目标。