An Amygdala to Ventrolateral Prefrontal Cortex Projection Implicated in Emotional Recognition

杏仁核到腹外侧前额叶皮层的投射与情绪识别有关

• 批准号: 10115523
• 负责人: Keshov Kumar Sharma
• 金额: $ 5.1万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-01 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10115523
• 关键词: Amygdaloid structure; Anatomy; Anger; Auditory; Automobile Driving; Basal Ganglia; Behavior; Brain region; Categories; Cell Nucleus; Cells; Characteristics; Chronic; Clip; Communications Media; Cues; Data; Depressed mood; Dimensions; Electrodes; Electrophysiology (science); Emotional; Emotions; Face; Facial Expression; Facial Expression Recognition; Fire - disasters; Foundations; Gestures; Grouping; Human; Implant; Individual; Injections; Investigation; Label; Lead; Learning; Libraries; Macaca; Macaca mulatta; Mental disorders; Modality; Modeling; Neurons; Patients; Pattern; Physicians; Population; Population Analysis; Prefrontal Cortex; Primates; Process; Psychiatry; Response Latencies; Scientist; Sensory; Signal Transduction; Site; Social Behavior; Social Functioning; Stimulus; Techniques; Testing; Tissues; Tracer; Training; Visual; Voice; base; career; design; experimental study; insight; movie; multisensory; neurophysiology; nonhuman primate; relating to nervous system; response; showing emotion; social; social communication; translational scientist; transmission process; vocalization

PROPOSAL SUMMARY – ABSTRACT Emotional expressions are an evolutionarily conserved form of communication and being able to recognize an emotion from a face or voice is a fundamental social function. Disturbances in emotional recognition are a common finding in many psychiatric diseases, but little is understood about the circuit processes that facilitate this behavior. Compared to neutral faces, viewing expressive faces (e.g. sad, angry, or happy faces) engages the amygdala and ventrolateral prefrontal cortex (VLPFC). Although the amygdala contains neurons that selectively respond to specific expressions, the overall activity of these neurons does not sort expressions into discrete categories. This suggests that the amygdala signals the salience of specific features but that it is not the site where categorization of these expressions occurs. In humans, the VLPFC is especially active when comparing facial expressions or matching expressions with descriptive words. In non-human primates, neurons in the VLPFC are highly responsive to faces and voices. Thus, the VLFPC may be a point where population activity categorizes expressions, but this has yet to be systematically tested. My preliminary data identifies VLPFC neurons with selective responses for expressions and a direct projection from the amygdala to the VLPFC, in the macaque. Building on these findings, this proposal seeks to combine anatomical tract-tracing, simultaneous dual-site multielectrode neurophysiology, and naturalistic, audiovisual expressions to test the hypothesis that the amygdala transmits information about the salient sensory features of emotional expressions directly to the VLPFC, where population activity segregates expressions into associated emotional categories. Using tissue from macaques with retrograde tracers placed at VLPFC sites that were highly responsive to faces and vocalizations, I will localize the origin of this projection in the amygdala and characterize it’s termination in complementary injections (Aim 1). Concurrently, I will implant multichannel electrodes into the amygdala and VLFPC to simultaneously record neuronal ensemble activity while macaques view movies of naturalistic, socio-emotional expressions i.e. facial gestures and associated vocalizations (Aim 2). By analyzing the sensory modalities that drive expression related responses, analyzing the population activity of these neurons, and comparing their response latencies between the amygdala and VLPFC, I will gain insight into the neuronal processing that occurs between these two regions to facilitate emotional recognition. In addition, learning to conduct anatomical and electrophysiological investigations into circuits for social behavior will serve as foundation for my intended a career as a translational researcher in psychiatry.

提案摘要 - 摘要 情感表达是一种进化的沟通形式，并承认安 脸部或声音的情感是一种基本的社会功能。 在许多精神疾病中的共同发现，但对有助于的电路过程几乎没有理解 这种行为与中立的面孔相​​比，观看表现出的面孔 杏仁核和腹侧皮层（VLPFC）。 有选择地响应特定表达式，这些神经元的整体活动不会将表达式分类为 离散类别。 这些表达式发生的站点在人类中，VLPFC特别活跃 在非人类灵长类动物中比较面部表情或匹配表达式。 在VLPFC中，对面孔和声音是高度的反应。 活动分类表达式，但这尚未进行系统的测试。 我的初步数据标识具有表达式和直接投影的选择性响应的VLPFC神经元 从杏仁核到VLPFC，在猕猴中。 解剖学追踪，同时双位点多电极神经生理学和自然主义，视听 表达以测试杏仁核传递有关显着感觉的信息的假设 情绪表达的特征直接传达给VLPFC，而人口活动则分离 使用猕猴带有反式示踪剂的组织中的表达 放置在对面孔和发声高度响应的VLPFC网站上，我将本地化的起源 在杏仁核中投影并在公司注射中的终止（AIM 1） 将植入杏仁核和VLFPC的多通道电极同时记录神经元集合 活动虽然猕猴会观看自然主义的电影，即社会情感表达方式，即面部手势 相关的发声（AIM 2）。 分析SE神经元的种群活动，并指南针其响应潜伏在您之间 Amygdala和VLPFC，我将深入了解十二个区域之间的神经元探测器 促进情感认可。 此外，学会为社会回路进行解剖学和电生理投资而进行。 行为将成为我作为精神病学转化研究人员的意图职业的基础。