A reference repository and database for natural isolates of Toxoplasma gondii

弓形虫天然分离株参考储存库和数据库

• 批准号: 10042835
• 负责人: CHUNLEI SU
• 金额: $ 7.21万
• 依托单位: UNIVERSITY OF TENNESSEE KNOXVILLE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-15 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10042835
• 关键词: Acquired Immunodeficiency Syndrome; Agriculture; Altitude; Animals; Biology; Birds; Blindness; Cell Culture Techniques; Cessation of life; Chronic; Clinical; Collection; Communities; Cryopreservation; Databases; Deposition; Disease Outbreaks; Encephalitis; Epidemiology; Evolution; Fetal Death; Fetal Development; Future; Genes; Genetic Variation; Genetic study; Genotype; Geographic Locations; Goals; Human; Immunocompetent; Immunocompromised Host; Individual; Infection; Intellectual functioning disability; Laboratories; Laboratory Research; Lead; Life; Location; Mammals; Mental disorders; Metadata; Mus; Parasites; Parasitic Diseases; Pathogenesis; Patients; Population; Pregnant Women; Public Health; Publications; Research; Resources; Restriction fragment length polymorphism; Sampling; Services; Tissues; Toxoplasma; Toxoplasma gondii; Toxoplasmosis; Virulence; chronic infection; foodborne; latent infection; material transfer agreement; programs; reactivation from latency; repository; transmission process; waterborne pathogen

Toxoplasma gondii, the agent of toxoplasmosis, has a global distribution in a broad spectrum of hosts including mammals and birds. It has been recognized as the major food- and water-borne pathogen in humans due to frequent outbreaks of infections. One-third of the human population worldwide is chronically infected. Infections in healthy pregnant women may cause blindness, intellectual disabilities, or even death of the fetuses. Reactivation of latent infection in immunocompromised patients, such as individuals with AIDS, can cause life- threatening encephalitis. Genetic studies from global samples elucidated high diversity in T. gondii parasites and the isolates vary markedly in virulence. The goal of this proposal is to establish a reference repository and database for natural isolates of T. gondii. From over 1,500 isolates that were previously obtained from different animal hosts and geographical regions in the USDA Animal Parasitic Disease Laboratory, we propose to revive and expand about four hundred representative isolates, and make them available to research community. These diverse parasite isolates are an invaluable resource for future study of transmission, pathogenesis, and evolution of virulence of T. gondii, and provide essential information to treat clinical toxoplasmosis.

弓形虫病的毒剂弓形虫弓形虫病在包括 哺乳动物和鸟类。由于 经常发生感染。全世界人口中有三分​​之一被长期感染。 健康孕妇的感染可能会导致失明，智力残疾甚至胎儿死亡。 免疫功能低下患者（例如患有艾滋病患者）的潜在感染重新激活可能会导致生命 - 威胁性脑炎。来自全球样品的遗传研究阐明了T. gondii寄生虫的高多样性 分离株的毒力明显变化。该建议的目的是建立一个参考存储库，并 T. gondii天然分离株的数据库。从以前从不同的不同的分离株中获得 美国农业部动物寄生虫疾病实验室中的动物宿主和地理区域，我们建议复兴 并扩展大约四百个代表性的分离株，并使其可用于研究社区。这些 多种寄生虫分离株是未来对传播，发病机理和进化的研究的宝贵资源 T. gondii的毒力，并提供了治疗临床弓形虫病的基本信息。