Genotyping Core

基因分型核心

• 批准号: 10011812
• 负责人: DANIEL J CARR
• 金额: $ 11.49万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10011812
• 关键词: Awareness; Breeding; Catalogs; Center Core Grants; Complex; Consumption; Core Facility; DNA; Data; Data Analyses; Exercise; Future; Gene Deletion; Generations; Genes; Genetic Polymorphism; Genetic Screening; Genotype; Housing; Human Resources; Inbred Mouse; Mus; Mutant Strains Mice; Mutation; Pattern; Process; Protocols documentation; Research; Research Personnel; Scientist; Services; Standardization; Statistical Data Interpretation; Time; Transgenic Organisms; Vision; Vision research; Work; cost; ranpirnase; vision science

ABSTRACT Genotyping Core The Genotyping Core provides investigators comprehensive services in genotyping a broad range of transgenic and mutant mouse strains in a fast and accurate manner that is critical to the analysis and interpretation of the data. The generation of conditional deletion of genes involves a complex breeding pattern and creates multiple lines. Accurate genotyping is vital to validate the successful targeted gene or genes to identify the final genotypes. Efficient and timely analysis of mouse genotypes greatly facilitates the management of PI mouse colonies and allows for the culling of unwanted genotypes in a timely fashion. This process expedites the PIs to manage their mouse lines and reduce the costs of housing. The Genotyping Core allows us to take much of the burden of genotyping multiple lines from lab personnel and to streamline and better maintain our mouse colonies. A second major advantage is the expertise of screening for genetic polymorphisms expressed in several inbred mouse lines that can impact the results of our work, e.g., rd1 and rd8 mutations and rpe65 polymorphisms. The Genotyping Core is especially helpful to the junior investigators who may not have this expertise in their lab and cannot afford to hire someone for this service. Furthermore, multiple genotyping requires different protocols to analyze the genotypes: different protocols are a time- consuming exercise. The core will standardize these protocols for the PI and provide support to scientists in their genotyping projects in every aspect of research, including planning, extracting DNA, genotyping, interpreting data, and doing statistical analysis.

抽象的 基因分型核心 基因分型核心为研究人员提供了全面的服务，以广泛的范围 以快速准确的方式转基因和突变小鼠菌株对分析至关重要 数据解释。基因有条件缺失的产生涉及复杂的繁殖模式 并创建多行。准确的基因分型对于验证成功的靶向基因或基因至关重要 确定最终基因型。小鼠基因型的有效和及时分析极大地促进了 PI小鼠菌落的管理，并允许及时淘汰不需要的基因型。这 处理加快了PI来管理其鼠标线并降低住房成本。基因分型核心 允许我们承担基因分型从实验室人员中的多条线的负担，并精简和简化和 更好地维护我们的小鼠菌落。第二个主要优势是筛查遗传的专业知识 多态性在几种近交小鼠系中表达，可能会影响我们工作的结果，例如RD1和RD1和 RD8突变和RPE65多态性。基因分型核心对初级调查人员特别有用 谁可能在实验室中没有这种专业知识，并且负担不起为此服务雇用某人。此外， 多种基因分型需要不同的方案来分析基因型：不同的方案是时间 - 消费运动。核心将标准化这些协议的PI，并为科学家提供支持 他们在研究的各个方面的基因分型项目，包括计划，提取DNA，基因分型， 解释数据并进行统计分析。