USING SYSTEMS BIOLOGY TO UNDERSTAND POLYGENIC CONTROL OF PRIMATE PUBERTY

利用系统生物学了解灵长类青春期的多基因控制

• 批准号: 7715953
• 负责人: Alejandro Lomniczi
• 金额: $ 2.77万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715953
• 关键词: Architecture; Complex; Computer Retrieval of Information on Scientific Projects Database; Developmental Process; Female; Funding; Gene Chips; Gene Expression Profile; Genes; Grant; Human; Hypothalamic structure; Institution; Macaca mulatta; Microarray Shared Resource; Monkeys; Neurosecretory Systems; Numbers; Primates; Protocols documentation; Puberty; Research; Research Personnel; Resources; Role; Sexual Development; Source; Systems Biology; Time; Tumor Suppression; United States National Institutes of Health; cDNA Arrays; concept; tumor necrosis factor receptor superfamily, member 10b protein, mouse

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The specific aim of this project is to use the newly developed rhesus macaque-specific Affymetrix gene chips (MK GeneChip) to provide proof of principle for the concept that the onset of primate puberty is associated with a coordinated change in expression of a network of genes previously known for their role in tumor suppression. A few years ago we employed a human cDNA array (OFISU Gene MicroArray Shared Resource) containing a limited number of probes (8,400; 75% corresponding to known genes) to interrogate the female monkey hypothalamus at the time of puberty. Although this study resulted in the identification of a group of genes potentially involved in the control of this developmental process, it is likely that only a fraction of the relevant genes were identified due to the small number of probes in the array, and the interspecies-hybridization protocol used, which especially limited our sensitivity. Since the new Affymetrix MK GeneChip contains the entire rhesus monkey transcriptome, and includes several probes corresponding to genes whose expression is known to increase at puberty, the proposed study should be instrumental in developing the first Systems Biology approach thus far attempted to dissect the complex architecture of the gene networks involved in the neuroendocrine control of primate sexual development.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 该项目的具体目标是利用新开发的恒河猴特异性 Affymetrix 基因芯片 (MK GeneChip) 为灵长类动物青春期的开始与基因网络表达的协调变化相关这一概念提供原理证明以前因其在肿瘤抑制中的作用而闻名。 几年前，我们使用包含有限数量探针（8,400 个；75% 对应于已知基因）的人类 cDNA 阵列（OFISU 基因微阵列共享资源）来询问青春期雌性猴子下丘脑。尽管这项研究鉴定出了一组可能参与控制这一发育过程的基因，但由于阵列中的探针数量较少，而且物种间的差异，很可能只鉴定了一小部分相关基因。使用杂交方案，这特别限制了我们的灵敏度。由于新的 Affymetrix MK 基因芯片包含整个恒河猴转录组，并且包括与已知在青春期表达增加的基因相对应的几个探针，因此拟议的研究应该有助于开发迄今为止试图剖析复杂结构的第一个系统生物学方法参与灵长类性发育神经内分泌控制的基因网络。