TAS::75 0849::TAS INCREMENTAL FUNDING

塔斯马尼亚州::75 0849::塔斯马尼亚州增量资金

• 批准号: 10038552
• 负责人: LEONARD FREEDMAND
• 金额: $ 4.87万
• 依托单位: LEIDOS BIOMEDICAL RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-31 至 2020-08-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10038552
• 关键词: Adherence; Aliquot; Animals; Biological; Blood; Blood specimen; Breast Cancer Risk Factor; CCR; Cancerous; Cell Culture Techniques; Cell Proliferation; Cells; Cervical; Clinical; Clinical Trials; Collection; Colon Carcinoma; Communication; DNA; Data Analyses; Databases; Diet; Differentiation and Growth; Flavonoids; Food; Freezing; Funding; Future; Generations; Genistein; Human; Human Resources; Imaging technology; In Vitro; Indole-3-Carbinol; Inflammation; Inflammatory; Joints; Laboratories; Leukocytes; Liquid substance; Maintenance; Measurement; Messenger RNA; Monitor; National Surgical Adjuvant Breast and Bowel Project; Natural regeneration; Nitrogen; Normal Cell; Physiological; Plasma; Process; Proteomics; Research; Resveratrol; Retrieval; Risk Behaviors; Rodent Model; Role; Sampling; Scientist; Services; Shipping; Specific qualifier value; Specimen; Stem cells; Structure of nail of toe; Sulfur; Technology; Testing; Tissue Sample; Tumor Stem Cells; Vial device; Visit; Woman; bean; biobank; men; neoplastic cell; repository; statistical center; stem cell differentiation; transcriptomics; tumor; tumor behavior

Support is part of a joint research initiative with CCR (Farrar Laboratory) to examine the role of various food components as modifiers of the behavior of tumor stem cells and ultimately on tumor regeneration. These in vitro studies use cell culture technologies to evaluate the influence of physiologically relevant concentrations of food components on the differentiation of cancerous and non-cancerous stem cells. Several biological measurements are made to evaluate the impact of indole 3-carbinol, genistein, resveratrol, allyl sulfur, etc. on stem cell differentiation and growth potential. This support is part of a joint research initiative with CCR (Drs. Timothy Veenstra and Thomas Turbyville Laboratories) to examine use proteomic technologies to evaluate the role of various food components as modifiers of breast cancer risk and tumor behavior. Both in vitro studies using cells in culture and animal studies will be used to evaluate food components. Initially studies will focus on genistein as a modifier of both normal and tumor cells in culture. Future studies will evaluate the influence of indole 3-carbinol, resveratrol on shifts in proteomics and how these relate to cell proliferation. This support is part of a joint research initiative with CCR (Colburn Laboratory) to examine the role of various food components as modifiers of inflammation and ultimately the impact on colon cancer. Animal studies which incorporate imaging technologies are used to monitor the impact of diet. Several biological measurements are made to evaluate the impact of indole 3-carbinol, genistein, resveratrol, flavonoids in beans, etc. on the inflammatory process. Fisher Bio Services receives clinical samples from contributing labs, aliquots specified samples to construct reference sets, logs identifiers for each sample, into their database, maintains freezers all these samples and sends out reference sets to scientists approved for testing. This will be an ongoing project as long as samples remain in the freezers. Storage and retrieval as needed of blood and cervical cell samples that were collected during clinical trial � ALTS. Cervical vials are stored in a cold room. Other blood and cervical samples are stored in liquid nitrogen freezers. This support is to reimburse Frederick for completing transcriptomic (mRNA) analysis from samples from human being and rodent models. Blood and tissue samples will be made available to those conducting the transcriptomic analysis. Most of the samples provided will require initial extraction. The data from these analyses are provided to us electronically for independent analysis. The SELECT Biorepository is a collection of blood, toenails, white cells, and plasma from the SELECT clinical trial involving over 35,000 men. The ongoing activities include processing blood on 2500 men seen on transition visits, storage of all the bloods since the study initiation in 2000, weekly generation of BSI files, ongoing communication with SELECT Statistical Center personnel, shipping samples fro the adherence sub-study, DNA extraction for roughly 400 specimens. The NSABP Repository for STAR includes ongoing storage of specimens and the maintenance of the freezers that store blood, white cells and plasma on over 19,000 women on the STAR clinical trial. The tasks involve regular checks of specimens and freezers, accession of specimens, record storage, and interaction with the NSABP.

支持是与CCR（Farrar实验室）共同研究计划的一部分，以研究各种食物成分作为肿瘤干细胞行为的修饰符以及最终在肿瘤再生中的作用。这些体外研究使用细胞培养技术来评估物理相关浓度的食物成分对可取消和非癌干细胞分化的影响。进行了几种生物学测量，以评估吲哚3-甲醇，染料木黄酮，白藜芦醇，烯丙基硫等对干细胞分化和生长潜力的影响。这种支持是与CCR（Timothy Veenstra博士和Thomas Turbyville Laboratories）进行联合研究计划的一部分，以研究使用蛋白质组学技术来评估各种食物成分作为乳腺癌风险和肿瘤行为的修饰符的作用。使用细胞在培养和动物研究中使用细胞的体外研究都将用于评估食物成分。最初的研究将集中于染料木黄酮作为培养中正常和肿瘤细胞的修饰剂。未来的研究将评估吲哚3-甲醇，白藜芦醇对蛋白质组学转移以及这些影响与细胞增殖的影响。这种支持是与CCR（Colburn Laboratory）共同研究计划的一部分，以研究各种食物成分作为炎症的修饰符，并最终对结肠癌的影响。合并成像技术的动物研究用于监测饮食的影响。进行了几种生物学测量，以评估吲哚3-甲醇，染料木黄酮，白藜芦醇，豆类中的类黄酮等对炎症过程的影响。 Fisher Bio Services从贡献实验室，等分试样的样品中接收临床样本，以构建参考集，每个样本的日志标识符，在其数据库中，将所有这些样品保存到其数据库中，并将参考集发送给获得测试的科学家。只要样品保留在冰柜中，这将是一个正在进行的项目。根据需要在临床试验中收集的血液和宫颈细胞样品的储存和检索。宫颈小瓶存放在寒冷的房间中。其他血液和宫颈样品存储在液氮冷冻剂中。这种支持是为了偿还弗雷德里克（Frederick）完成从人类和啮齿动物模型的样品中完成转录组（mRNA）分析。进行转录组分析的人将提供血液和组织样本。提供的大多数样品都需要初始提取。这些分析中的数据以电子方式提供给美国以进行独立分析。精选的生物座是来自涉及35,000多名男性的精选临床试验的血液，脚趾甲，白细胞和血浆的集合。正在进行的活动包括在过渡访问中看到的2500名男性处理血液，自2000年研究计划以来所有血液的存储，每周的BSI文件，与某些统计中心人员进行的持续沟通，通过依从性子研究，DNA依次提取大约400个标本。 NSABP恒星存储库包括持续存储标本以及在Star Clinical试验中存储19,000多名女性的血液，白细胞和血浆的冰柜维护。这些任务涉及定期检查标本和冰柜，标本的登录，记录存储以及与NSABP的交互。