Genetic dissection of motivational drive to eat

进食动机的基因剖析

基本信息

批准号：
10028237
负责人：
Qili Liu
金额：
$ 40.38万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2025-06-30
项目状态：
未结题

项目摘要

Project Summary Food intake can be triggered by not only the need for calories, but also the need for specific nutrients, or need-independent mechanisms. Studies over the recent decades have made substantial progress in our understanding of neural pathways controlling food intake in the context of energy homeostasis. In contrast, mechanisms underlying calorie-independent feeding remain poorly understood. Using Drosophila as a model system, I recently identified and characterized the first neural circuit encoding protein-specific hunger in any model system. This circuit provides a novel entry for future investigations into the nutrient specific food intake that is independent of caloric needs. In this proposed study, I will characterize the molecular and cellular substrates mediating the homeostatic regulation of protein consumption, with a particular focus on the sensing of protein abundance or scarcity. We also plan to elucidate how environmental blue light drives feeding behavior in the absence of nutritional needs. To achieve these goals, we will employ a multidisciplinary approach including large-scale genetic analyses, quantitative behavioral measurements, immunohistochemistry, functional imaging, patch- clamp electrophysiology and metabolomics analysis. Together these investigations will substantially deepen our understanding of calorie-independent motivational drive to eat, shedding light on the fundamental principles for the organization and modulation of feeding behaviors.

项目摘要食物摄入量不仅可以是由于对卡路里的需求而触发的，而且还需要对特定营养素的需求，或独立的机制。近几十年来的研究取得了重大进展我们对能量稳态中控制食物摄入的神经途径的理解。在对比度，与卡路里无关的喂养的基础机制保持不足。使用果蝇作为模型系统，我最近确定并表征了第一个神经回路编码任何模型系统中的蛋白质特异性饥饿。该电路为未来的调查提供了新的条目进入独立于热量需求的营养特异性食物摄入量。在这项拟议的研究中，我将表征介导蛋白质稳态调节的分子和细胞底物消费，特别关注蛋白质丰度或稀缺性的感知。我们还计划在没有营养需求的情况下，阐明环境蓝光如何驱动喂养行为。到实现这些目标，我们将采用多学科方法，包括大规模遗传分析，定量行为测量，免疫组织化学，功能成像，斑块 - 夹具电生理学和代谢组学分析。这些调查将大大将加深我们对与卡路里无关的饮食动机的理解，阐明组织和调制喂养行为的基本原则。