Mays Cancer Center at UT Health SA

UT Health SA 梅斯癌症中心

基本信息

批准号：
10025091
负责人：
Zhao Lai
金额：
$ 8.47万
依托单位：
UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-01 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10025091
关键词：
Advisory Committees Applications Grants Area Base Sequence Bioinformatics Bioinformatics Shared Resource Biological Assay Biometry CRISPR screen Cancer Center Cancer Center Support Grant Catchment Area Cells ChIP-seq Child Chromosome Structures Collaborations Complement Consultations Copy Number Polymorphism Custom DNA Damage DNA copy number DNA sequencing Data Diagnosis Doctor of Philosophy Educational workshop Environment Equipment Experimental Designs Faculty Funding Funding Agency Future Gene Expression Gene Expression Regulation Generations Genomics Goals Group Meetings Health Journals Knowledge Libraries Link Malignant Neoplasms Manuscripts Metagenomics MicroRNAs Mission Mutation Nature Pathway interactions Peer Review Phenotype Population Preparation Price Protocols documentation Publications Reagent Research Research Institute Research Personnel Resource Sharing Sampling Screening for cancer Services Small RNA Statistical Data Interpretation Strategic Planning Students Technology Training Tumor Subtype Tumor Suppressor Proteins Variant Work anticancer research cancer cell cancer heterogeneity cancer initiation chromatin modification cost effective data pipeline design direct application experimental study genomic data improved liquid biopsy member next generation sequencing novel outcome forecast programs single cell analysis square foot tool transcriptome sequencing tumor heterogeneity

项目摘要

Project Summary—Next Generation Sequencing Shared Resource The mission of the Next-Generation Sequencing Shared Resource (NGSSR) is to provide Mays Cancer Center (MCC) members with state-of-the-art next generation sequencing technology for detecting and exploring alterations in nucleotide sequence, DNA copy number variation, gene expression differences, chromosome structure variation, chromatin modification and all interactions which are linked to the complexity of phenotypes seen in cancers. The NGSSR, directed by Zhao Lai, Ph.D., has been an MCC Shared Resource since 2012. It is located in 1,000 sq. ft. of space in the Greehey Children’s Cancer Research Institute (GCCRI) building, adjacent to the MCC. Dr. Lai is assisted by technical director Ms. Dawn Garcia. The NGSSR conducts sequence applications for MCC users across all 3 scientific programs. Services include various types of library construction, DNA-seq, RNA-seq, ChIP-seq, small RNA-seq and exomeCap-seq, CRISPR screening, 16S metagenomics, and single cell analysis. In addition, Dr. Lai and the NGSSR team assist users with experimental design and provide educational training to faculty, staff and students. The NGSSR works closely with the Biostatistics and Bioinformatics Shared Resource (BBSR), led by Yidong Chen, Ph.D. (PSP), a bioinformatics expert. The BBSR works with the NGSSR to provide MCC members with computational capabilities that complement the NGSSR’s offerings. The NGSSR is overseen by a Scientific Advisory Committee and the MCC Shared Resource Oversight Committee (SROC). In 2018, the NGSSR was used by 31 peer-reviewed funded MCC members (36% of total usage) and contributed to 12 cancer-related manuscripts in 2018. Since its inception, from 2014 to the middle of 2018, NGSSR has quickly become an essential component in MCC and transformed the genomics research environment for MCC members with a nearly three-fold increase in the number of users and contributions to over 44 cancer-related publications in NEJM, Nature, Cancer Cell, PNAS, and other high-impact peer-reviewed journals. As new tools and reagents are developed (e.g. liquid biopsies) they can be readily implemented in NGS assays. Another exciting area of cancer research is single-cell analysis. The NGSSR will be heavily involved in strategic planning and design of these experiments. The NGSSR has improved efficiency, created a smooth data pipeline incorporating analyses, and acquired new hardware through multiple funding sources to upgrade its equipment. It continues to provide essential cutting-edge, high-quality, and cost-effective services to MCC users across all 3 scientific programs.

项目摘要 - 次生成测序共享资源下一代测序共享资源（NGSSR）的任务是提供Mays癌症中心（MCC）具有最先进的下一代测序技术用于检测和探索的成员核苷酸序列的改变，DNA拷贝数变化，基因表达差异，染色体结构变化，染色质修饰和与表型复杂性相关的所有相互作用在癌症中看到。自2012年以来，由Zhao Lai博士执导的NGSSR一直是MCC共享资源。它位于希腊儿童癌症研究所（GCCRI）大楼的1,000平方英尺中毗邻MCC。莱博士在技术主管Dawn Garcia女士的协助下。 NGSSR进行在所有3个科学计划中，MCC用户的序列应用程序。服务包括各种类型的库构造，DNA-Seq，RNA-Seq，Chip-Seq，小RNA-Seq和ExomeCap-Seq，CRISPR筛选，16S 宏基因组学和单细胞分析。此外，LAI博士和NGSSR团队协助用户实验设计并为教职员工和学生提供教育培训。 NGSSR与由钦（Yidong Chen）领导的生物统计学和生物信息学共享资源（BBSR）博士。（PSP），一种生物信息学专家。 BBSR与NGSSR合作，为MCC成员提供计算功能补充NGSSR的产品。 NGSSR由科学咨询委员会和MCC监督共享资源监督委员会（SROC）。 2018年，NGSSR由31个同行评审资助使用 MCC成员（占总用法的36％），并在2018年贡献了12项与癌症相关的手稿。 Inception，从2014年到2018年中，NGSSR迅速成为MCC和MCC的重要组成部分改变了MCC成员的基因组研究环境，增加了近三倍在NEJM，Nature，Nature，Cancer Cell，PNAS，和其他高影响同行评审的期刊。随着新工具和试剂的开发（例如液体活检）它们可以在NGS测定中很容易实施。癌症研究的另一个令人兴奋的领域是单细胞分析。 NGSSR将大量参与这些实验的战略计划和设计。 NGSSR拥有提高效率，创建了平稳的数据管道编码分析，并通过多个资金来源来升级其设备。它继续提供重要的尖端，高质量的以及所有3个科学计划的MCC用户的成本效益服务。