The intersection of development and innate immune system function in Arabidopsis

拟南芥发育与先天免疫系统功能的交叉点

• 批准号: 10001536
• 负责人: JEFFERY L. DANGL
• 金额: $ 26.59万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10001536
• 关键词: Affect; Animals; Antirrhinum; Arabidopsis; Biochemical; Biological Process; Biology; Cell physiology; Cells; Cellular biology; Complex; Conflict (Psychology); Cues; Data; Development; Differentiation and Growth; Eating; Ecosystem; Equilibrium; Eukaryota; Evolution; Family; Family member; Fiber; Fostering; Funding; Gene Family; Genes; Genetic; Genetic Polymorphism; Genetic Transcription; Genomics; Goals; Growth; Growth and Development function; Health; Home environment; Hormonal; Host Defense; Human; Immune; Immune response; Immune system; Immunity; Immunologic Monitoring; Immunologic Receptors; Infection; Innate Immune Response; Innate Immune System; Insecta; Investigation; Life; Life Style; Maize; Mediating; Modeling; Molecular; Mosses; Mutation; Natural Immunity; Normal Cell; Oomycetes; Oxygen; Personal Satisfaction; Physiology; Phytoplasma; Plant Growth Regulators; Plants; Process; Productivity; Proliferating Cell Nuclear Antigen; Proteins; Recording of previous events; Regulon; Reporting; Research; Research Support; Resource Allocation; Resources; Rice; Signal Transduction; Specificity; Stress; Structure; System; United States National Institutes of Health; Virulence; Virulence Factors; Zea luxurians; base; cellular targeting; extracellular; feeding; fitness; genome-wide; human pathogen; immune function; immune system function; interest; member; novel; pathogen; pathogenic microbe; plant growth/development; programs; receptor; receptor function; reproductive success; response; symbiont; transcription factor

PROJECT ABSTRACT Plants are critical for human health and well being. We eat plants, or animals that ate plants before we ate them; we use plant fibers for our clothes and our homes; we rely on plants to provide ecosystems conducive to environmental well being. Plants provide us with oxygen. Without plants, human life would be impossible. Hence, research to understand plant growth, health and productivity is explicitly relevant to human health and well being, as stressed in the 2009 NRC report: `A New Biology for the 21st Century'. Plant research contributes significantly to understanding of basic processes in humans. Relevant to this proposal is the finding that the intracellular receptors that are major regulators of the plant immune system are structurally and functionally analogous to similar receptors functioning in animal innate immune systems. The experimental ease of Arabidopsis genetics, genomics, and cell biology leads to discoveries about fundamental processes shared across all eukaryotes, especially those that cross reference normal development with a host's response to microbial pathogens, the focus of this proposal. This project takes advantage of completed and current NIH supported research revealing how the effector protein (virulence factor) repertoires from a bacterial, a fungal and a eukaryotic oomycete pathogen converge onto an interconnected set of intracellular host targets. This convergence is striking as these three pathogens are separated by ~2 billion years of evolution and have very different life styles and virulence mechanisms. These data supported the overall hypothesis that pathogens usurp normal developmental and cell biological processes to counteract host immune responses. The long term goal of this research is to understand the functional processes of development and immunity governed by a subset of ancient and conserved transcription factors, called TCPs, that are repeatedly targeted by diverse pathogen effectors, and that form a tight sub-network in the current Arabidopsis interactome. TCP proteins are well-characterized regulators of development, but novel players in defense. This competitive renewal proposal will dissect the molecular mechanism of transcriptional coordination across conflicting developmental and defense cues. TCP genes are an ancient gene family found in pteridophytes, lycophytes, moss and some algal species, representing an evolutionary history of about 650 million years. This enables study of the co-evolution of TCP protein developmental and immune functions. We are specifically interested in understanding how a subset of TCP proteins controls the intersection of normal cell signaling for growth and development and how the repeated evolution of diverse effectors that target these TCPs manipulates the regulons they control to favor the proliferation of pathogens with diverse lifestyles. This research will benefit investigations of animal pathogens, since effector proteins from human pathogens also manipulate normal host cell physiology by targeting critical regulators of normal cell function.

项目摘要 植物对人类健康和福祉至关重要。我们吃植物，或者在我们吃饭之前先吃植物的动物 他们;我们在衣服和家居中使用植物纤维；我们依靠植物提供有利于人类生存的生态系统 环境福祉。植物为我们提供氧气。没有植物，人类的生命就不可能存在。 因此，了解植物生长、健康和生产力的研究与人类健康和生产力明确相关。 正如 2009 年 NRC 报告中所强调的那样：“21 世纪的新生物学”。 植物研究对理解人类的基本过程做出了重大贡献。与此相关 该提案的发现是，作为植物免疫系统主要调节因子的细胞内受体是 在结构和功能上类似于动物先天免疫系统中起作用的类似受体。这 拟南芥遗传学、基因组学和细胞生物学的实验简便性导致了关于基本原理的发现 所有真核生物共有的过程，特别是那些将正常发育与真核生物交叉引用的过程 宿主对微生物病原体的反应是该提案的重点。 该项目利用了 NIH 支持的已完成和当前研究，揭示了效应器如何 来自细菌、真菌和真核卵菌病原体的蛋白质（毒力因子）库汇聚在一起 到一组相互连接的细胞内宿主靶标上。这种趋同是惊人的，因为这三种病原体 它们相距约 20 亿年的进化历程，并且具有截然不同的生活方式和毒力机制。 这些数据支持了病原体侵占正常发育和细胞生物学的总体假设 抵消宿主免疫反应的过程。 这项研究的长期目标是了解发育和免疫的功能过程 由一组古老且保守的转录因子（称为 TCP）控制，这些转录因子被反复靶向 由不同的病原体效应子组成，并在当前的拟南芥相互作用组中形成一个紧密的子网络。传输控制协议 蛋白质是众所周知的发育调节因子，但也是防御中的新参与者。 这项竞争性更新提案将剖析跨转录协调的分子机制 冲突的发展和防御线索。 TCP基因是蕨类植物中发现的一个古老的基因家族， 石松类、苔藓类和一些藻类物种，代表了大约 6.5 亿年的进化历史。这 能够研究 TCP 蛋白发育和免疫功能的共同进化。我们专门 有兴趣了解 TCP 蛋白的子集如何控制正常细胞信号传导的交叉 生长和发育以及针对这些 TCP 的不同效应器如何重复进化 操纵它们控制的调节子以促进具有不同生活方式的病原体的增殖。 这项研究将有利于动物病原体的研究，因为来自人类病原体的效应蛋白 还通过针对正常细胞功能的关键调节因子来操纵正常宿主细胞的生理机能。

项目成果

Convergent targeting of a common host protein-network by pathogen effectors from three kingdoms of life.

• DOI: 10.1016/j.chom.2014.08.004
• 发表时间: 2014-09-10
• 期刊: Cell host & microbe
• 影响因子: 30.3
• 作者: Weßling R;Epple P;Altmann S;He Y;Yang L;Henz SR;McDonald N;Wiley K;Bader KC;Gläßer C;Mukhtar MS;Haigis S;Ghamsari L;Stephens AE;Ecker JR;Vidal M;Jones JD;Mayer KF;Ver Loren van Themaat E;Weigel D;Schulze-Lefert P;Dangl JL;Panstruga R;Braun P
• 通讯作者: Braun P

LSU network hubs integrate abiotic and biotic stress responses via interaction with the superoxide dismutase FSD2.

• DOI: 10.1093/jxb/erw498
• 发表时间: 2017-02-01
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Garcia-Molina A;Altmann M;Alkofer A;Epple PM;Dangl JL;Falter-Braun P
• 通讯作者: Falter-Braun P