Physical modeling of biological systems

生物系统的物理建模

• 批准号: 8746533
• 负责人: peter j munson
• 金额: $ 17.16万
• 依托单位: CENTER FOR INFORMATION TECHNOLOGY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8746533
• 关键词: Address; Affect; Anemia; Base Sequence; Binding; Biology; Biomedical Engineering; Biomedical Research; Bioreactors; Blood capillaries; Cell Count; Cells; Cellular biology; Chicago; Chromosomes; Development; Drosophila genus; Dyes; Engineering; Erythrocytes; Eukaryota; Exposure to; Film; Frequencies; Genes; Growth; Heating; Hemoglobin; Hot Spot; Human; Immune response; Information Technology; International; Investigation; Laboratories; Legal patent; Libraries; Light; Liquid substance; Malaria; Mathematics; Mediation; Metabolism; Methods; Microdissection; Modeling; Molecular Medicine; National Cancer Institute; National Institute of Allergy and Infectious Disease; National Institute of Biomedical Imaging and Bioengineering; National Institute of Child Health and Human Development; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Mental Health; Neurons; Oxygen; Paper; Parasites; Pathology; Patients; Pattern; Peer Review; Polymers; Population Dynamics; Population Study; Positioning Attribute; Process; Proteins; Publishing; Research; Research Personnel; Resource Sharing; Retrotransposon; Staining method; Stains; Statistical Models; Techniques; Technology Transfer; Thalassemia; Tissues; United States National Institutes of Health; Universities; Visit; Work; biological systems; capillary; cell growth regulation; computer science; engineering design; epidemiology study; fitness; genome analysis; international center; mathematical model; melting; neurotoxicology; novel; operation; physical model; physical science; professor; programs; reproductive; retinal neuron; statistics; transmission process; vector

With an investigator in the Division of International Epidemiology and Population Studies, Fogarty International Center, and with a professor from Loyola University Chicago visiting the Laboratory of Malaria and Vector Research, NIAID, we have developed a phenomenological model of population dynamics of the transmissible form of the malaria parasite, the gametocytes. We showed that for the same ability to attack and remove targets, host immune responses against the immature, non-transmissible gametocytes would affect transmission more than immune responses directly against the mature, transmissible forms. A peer-reviewed paper about this work has been published With a consortium of investigators from (1) Program in Physical Biology, NICHD, (2) Molecular Medicine Branch, NIDDK, and (3) Laboratory of Malaria and Vector Research, NIAID, we are studying the affects of human hemoglobin abnormalities on the reproductive fitness of malaria parasites. We have demonstrated experimentally that malaria parasites reproduce at a lower rate in erythrocytes from patients with Thalassemia anemia. A paper is currently under referee review. With a consortium of investigators from (1) Laboratory of Neurotoxicology, National Institute of Mental Health, (2) Program in Physical Biology, (NICHD), (3) Laboratory of Pathology, National Cancer Institute, (4) Computational Bioscience and Engineering Laboratory, Division of Computational Bioscience, Center for Information Technology, (5) Biomedical Engineering and Physical Science Shared Resource, National Institute of Biomedical Imaging and Bioengineering, we modeled the thermal and fluid transport that occur in the operation of activated expression microdissection. This is a method of extracting large number of cells in which a normally expressed protein is stained with a light-absorbing dye. At exposure to light, the heated stain melts a polymer film that binds to tissue, allowing for pickup of cellular components. The engineering development is nearing the point where the method can be utilized in pathology laboratories, and the NIH Office of Technology Transfer is in negotiations with potential commercial developers. With an investigator in Laboratory of Gene Regulating and Development, NICHD, we are working on statistical and topological analysis of retinal neurons growth in Drosophila. Distinct patterns of dendritic branching are associated with expression of certain genes in such neurons, but we have found that dendritic branching is not a simple Poisson process. A paper describing this work is currently under referee review. With a consortium of investigators from (1) Laboratory of Cell Biology, NCI, and (2) Computational Bioscience and Engineering Laboratory, Division of Computational Bioscience, CIT, and (3) Biomedical Engineering and Physical Science Shared Resource, National Institute of Biomedical Imaging and Bioengineering, we did engineering design of a bioreactor for growing cells in a 3D matrix with capillary-like components for delivery of oxygen. A peer-reviewed paper describing this apparatus has been published, and a patent application submitted. With a researcher in the Program in Cellular Regulation and Metabolism, NICHD, we are studying the frequency at which retrotransposons insert themselves into eukaryote chromosomes. A library of specially engineered nucleotide sequences are attached to the retrotransposons, allowing for a statistical analysis of genome patterns around hot spots, positions on chromosomes where insertion occur at a high rate.

借助Fogarty International Center的国际流行病学和人口研究部研究人员，芝加哥洛约拉大学的教授访问了NIAID的疟疾和媒介研究实验室，我们开发了一种对疟原虫寄生虫的易跨性形式的人口动态的现象学模型。我们表明，对于相同的攻击和删除目标的能力，宿主免疫反应针对未成熟，不可允许的配子细胞会影响传播，而不是直接影响免疫反应，直接与成熟的，可传播的形式有关。关于这项工作的同行评审论文已发表 借助（1）个物理生物学计划的研究人员，NICHD，（2）分子医学分支，NIDDK和（3）NIAID的疟疾和载体研究实验室，我们正在研究人类血红蛋白异常对疟疾帕拉斯群岛生殖适应性的影响。我们在实验上证明了疟疾寄生虫在贫血贫血患者的红细胞中以较低的速度繁殖。目前正在裁判审查中论文。 With a consortium of investigators from (1) Laboratory of Neurotoxicology, National Institute of Mental Health, (2) Program in Physical Biology, (NICHD), (3) Laboratory of Pathology, National Cancer Institute, (4) Computational Bioscience and Engineering Laboratory, Division of Computational Bioscience, Center for Information Technology, (5) Biomedical Engineering and Physical Science Shared Resource, National Institute of Biomedical Imaging and Bioengineering, we模拟了激活表达显微解剖运行中发生的热和流体转运。这是一种提取大量细胞的方法，其中正常表达的蛋白质用浅色染料染色。暴露于光线时，加热的染色融化了与组织结合的聚合物膜，从而可以拾取细胞成分。工程开发接近可以在病理实验室中使用该方法的地步，而NIH技术转移办公室正在与潜在的商业开发商进行谈判。 NICHD的基因调节和发育实验室研究人员，我们正在研究果蝇视网膜神经元生长的统计和拓扑分析。树突分支的不同模式与此类神经元中某些基因的表达有关，但我们发现树突分支并不是一个简单的泊松过程。描述这项工作的论文目前正在裁判审查中。 与（1）来自（1）细胞生物学，NCI和（2）计算生物科学和工程实验室实验室的研究人员，计算生物科学，CIT和（3）生物医学工程和物理科学共享资源，国家生物医学成像研究所和生物工程研究所的生物医学研究所，我们为生物工程的生物工程设计了，我们在三大的工程设计中，用于培养的capillix capill capill capill of Bioreactor capill of Bioreagrix capill of Bioreagrix of 3d dd caplix in 3d。氧气。一份由同行评审的论文描述了该设备，并提交了专利申请。 NICHD中，随着细胞调节和代谢方面的研究人员的研究，我们正在研究逆转录座子将自己插入真核生物染色体的频率。专门工程的核苷酸序列库附着在逆转录座子上，允许对热点周围的基因组模式进行统计分析，在染色体上的位置，插入以高速率发生。