National User Resource for Biological Accelerator Mass Spectrometry

生物加速器质谱国家用户资源

• 批准号: 10021178
• 负责人: Graham Bench
• 金额: $ 140.6万
• 依托单位: LAWRENCE LIVERMORE NATIONAL SECURITY, LLC
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10021178
• 关键词: Address; Animal Model; Area; Award; Biochemistry; Biological; Biological Models; Biological Sciences; Biomedical Research; Biomedical Technology; Biophysics; Budgets; C14 isotope; Cells; Cellular biology; Code; Communities; Complex; Computer software; Data Analyses; Data Reporting; Detection; Development; Developmental Biology; Disease; Drug Kinetics; Etiology; Funding; Gases; Genetic; Goals; Grant; Health; Human; Isotopes; Label; Laboratories; Lipids; Mass Spectrum Analysis; Measurement; Measures; Metabolic; Metabolic Pathway; Metabolism; Methods; Modeling; Modification; Molecular; Molecular Biology; National Institute of General Medical Sciences; Organism; Pathway interactions; Pharmacology; Physiological; Physiology; Property; Proteins; Radioisotopes; Radiolabeled; Research; Research Personnel; Research Support; Resource Development; Resources; Risk Assessment; Safety; Sampling; Science; Scientist; Services; System; Technology; Therapeutic; Toxicology; Training; United States; United States National Institutes of Health; Work; Workforce Development; Xenobiotic Metabolism; accelerator mass spectrometry; base; carcinogenesis; drug development; improved; in vivo; ion source; mass spectrometer; metabolic abnormality assessment; metabolic rate; nutrition; operation; programs; research study; tool; toxicant

PROJECT SUMMARY The National User Resource for Biological Accelerator Mass Spectrometry (User Resource) will provide ultra- sensitive isotopic analysis (primarily radiocarbon or Carbon-14) by accelerator mass spectrometry (AMS) for NIH-funded and other researchers. It will be the only User Resource of its type in the United States. The User Resource will leverage 20 years of expertise in the development and application of AMS in broad-based biomedical research through the Biomedical Technology Research Resource for the Development of Biomedical Accelerator Mass Spectrometry (BTRRAMS): a NIGMS Biomedical Technology Center (P41GM103483 09/01/2000 – 05/31/2020) that is in its final year of operation. Since June 2014, the BTRRAMS has measured over 20,000 samples (4,000 a year) a year for its user community. The User Resource will continue to meet the needs of current users as well as provide a capability for new users who would benefit from incorporating AMS as a measurement tool into their research programs. The User Resource’s forte will be ultra-high sensitivity quantitation of radiocarbon and selected other radioisotopes for research studies where isotopes are required. Radioisotope labeling studies continue to be an important tool for addressing many complex problems in the biomedical sciences. AMS is a specialized and unique type of mass spectrometry that provides absolute quantitation of radiocarbon and relevant other isotopes with limits of detection in real samples on the order of a few attograms/mg sample. As a National Resource, the most significant work enabled by AMS is likely to be quantification of metabolic pathways in human health states and comparison to ex vivo and in vivo animal models. The technology made available will enable a deeper understanding of the etiology of human health concerns by (1) enabling the ability to quantify pharmacokinetics and other molecular endpoints directly in humans; (2) offering the ability to conduct quantitative studies using biologics such as proteins or lipids; and (3) enabling more relevant studies of metabolic pathways in health and disease through the use of much lower, more biologically-relevant, concentrations of metabolic substrates in cells and intact organisms. Such studies support NIGMS’s basic biomedical research areas that contribute to the understanding of fundamental cellular and physiological principles. The User Resource has three goals: (1) provide high throughput, ultra-sensitive 14C analysis for the NIH user community ; (2) improve the efficiency of ultra-sensitive 14C analysis through installation of new interfaces to our AMS systems, improvements in ion source efficiency and upgrades to our data analysis codes; and (3) increase the accessibility and visibility of ultra-sensitive 14C measurements for the biomedical research community by training new investigators and expanding the National user base of researchers requiring ultra-sensitive 14C analysis.

项目概要 国家生物加速器质谱用户资源（用户资源）将提供超 通过加速器质谱 (AMS) 进行敏感同位素分析（主要是放射性碳或碳 14） NIH 资助的和其他研究人员将是美国同类的唯一用户资源。 Resource 将利用 20 年在广泛领域的 AMS 开发和应用方面的专业知识 通过生物医学技术研究资源进行生物医学研究，以促进 生物医学加速器质谱 (BTRRAMS)：NIGMS 生物医学技术中心 (P41GM103483 09/01/2000 – 05/31/2020) 自 2014 年 6 月以来，这是其运营的最后一年。 BTRRAMS 每年为其用户社区测量超过 20,000 个样本（每年 4,000 个）。 资源将继续满足当前用户的需求，并为新用户提供功能 将 AMS 作为测量工具纳入他们的研究计划中将会受益。 Resource 的强项是对放射性碳和选定的其他放射性同位素进行超高灵敏度定量 需要同位素标记研究的研究仍然是一个重要工具。 AMS 是一种专门且独特的类型，用于解决生物医学科学中的许多复杂问题。 质谱法可提供放射性碳和相关其他同位素的绝对定量（有限制） 作为国家资源，实际样品中的检测量约为几阿克/毫克样品。 AMS 促成的重要工作可能是对人类健康状态的代谢途径进行量化 以及与离体和体内动物模型的比较。所提供的技术将有助于进行更深入的研究。 通过以下方式了解人类健康问题的病因：(1) 能够量化 直接在人体中进行药代动力学和其他分子终点；（2）提供进行的能力 使用蛋白质或脂质等定量研究进行研究；(3) 进行更相关的研究 通过使用低得多、生物学相关性更高的代谢途径来研究健康和疾病的代谢途径 这些研究支持 NIGMS 的基本原理。 有助于理解基本细胞和生理学的生物医学研究领域 用户资源具有三个目标：(1) 提供高通量、超灵敏的 14C 分析。 NIH 用户社区；(2) 通过安装新的系统来提高超灵敏 14C 分析的效率 与我们的 AMS 系统的接口、离子源效率的改进以及数据分析的升级 (3) 提高生物医学领域超灵敏 14C 测量的可及性和可视性 通过培训新研究人员和扩大研究人员的全国用户群来研究社区 需要超灵敏的 14C 分析。