Genetic Studies of the Synapse

突触的遗传学研究

基本信息

批准号：
8018406
负责人：
LILY Y JAN
金额：
$ 37.99万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-09-01 至 2016-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8018406
关键词：
1-Phosphatidylinositol 3-Kinase Action Potentials Adopted Ankyrins Area Autistic Disorder Award Axon Binding Binding Proteins Brain COX7A2L Protein Calcium Calcium Spikes Cell membrane Chloride Channels Convulsions Coupling Dendrites Distal Endoplasmic Reticulum Epilepsy Family Feedback Fluorescence Freedom Generations Genes Genetic Genetic Translation Glutamate Receptor Goals Golgi Apparatus Grant Growth Hippocampus (Brain)Inherited Instruction Ion Channel KRP protein Kinesin Kinetics Learning Legal patent Mammalian Cell Mediating Membrane Membrane Potentials Mental Retardation Mental disorders Messenger RNA Microtubule Bundle Microtubule-Associated Proteins Microtubules Modeling Motor Movement Mutant Strains Mice Mutation Myokymia N-Methyl-D-Aspartate Receptors N-Methylaspartate Neonatal Neurons Paper Phosphotransferases Physiological Plants Plus End of the Microtubule Positioning Attribute Potassium Potassium Channel Property Protein Binding Proteins Publications Publishing Regulation Reporter Research Design Research Peer Review Risk Role Signal Pathway Signal Transduction Sirolimus Site Structure Suppressor Mutations Surface Synapses Synaptic Potentials Synaptic plasticity TSC1 gene TSC2 gene Testing Translations Tuberous Sclerosis Tuberous sclerosis protein complex Varicosity Vesicle Voltage-Gated Potassium Channel Xenopus oocyte Yeasts base density dimer experience expression cloning follow-up genetic regulatory protein information processing inhibitor/antagonist insight mTOR protein meetings mutant neuronal excitability novel response sensor synaptic function synaptic inhibition trafficking voltage

项目摘要

The long-term objectives are to study how potasslunn channels regulation contributes to neuronal signaling. The specific aims are to understand how the dendritic voltage-gated potassium channels and G proteinactivated inwardly rectifying potassium (GIRK) channels are regulated in hippocampal neurons, and how these potassium channels contribute to neuronal signaling and synaptic plasticity. The research design involves examination of mutant mice with altered or deleted potassium channel regulatory proteins, for regulation of either the local synthesis of channels in the dendrite or the activity or kinetic properties of the channel on the cell membrane. Because the Kv1 voltage-gated potassium channels reside on both dendrites and axons, the mosaic analysis with double markers (MADM) approach will be employed to study the physiological role of dendritic Kv1 channels.

长期目标是研究钾通道调节如何有助于神经元信号传导。具体目的是了解树突电压门控钾通道和G蛋白活化的方式向内整流钾（GIRK）通道受到海马神经元的调节，以及如何调节这些钾通道有助于神经元信号传导和突触可塑性。研究设计涉及检查突变小鼠，以改变或删除的钾通道调节蛋白，用于检查调节树突中通道的局部合成或活性或动力学特性细胞膜上的通道。因为KV1电压门控钾通道都位于两个树突上和轴突，将采用双重标记物（MADM）方法的镶嵌分析来研究树突状KV1通道的生理作用。