PROTEINS ASSOCIATED W/ CAVEOLAE FROM ADIPOCYTES

与脂肪细胞小窝相关的蛋白质

• 批准号: 7601968
• 负责人: PAUL F PILCH
• 金额: $ 0.11万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-03 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601968
• 关键词: Adipocytes; CD36 gene; Caveolae; Caveolins; Cell membrane; Complement; Computer Retrieval of Information on Scientific Projects Database; Electron Microscopy; Funding; Grant; Institution; Insulin; Insulin Receptor; Label; Link; Lipids; Lipoprotein Receptor; Metabolism; Monoclonal Antibodies; Play; Procedures; Proteins; Regulation; Research; Research Personnel; Resources; Role; Source; United States National Institutes of Health; caveolin 1; cell type; diamino oxhydrase; flotillin; insulin signaling

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Adipocytes play an important role in the insulin-dependent regulation of organismal fuel metabolism and express caveolae at levels as high or higher than any other cell type. Recently, a link between insulin signaling and caveolae has been suggested. Nevertheless, adipocyte caveolae have been the subject of relatively few studies, and their contents have been minimally characterized. With the aid of a new monoclonal antibody, we developed a rapid procedure for the immunoisolation of caveolae derived from the plasma membrane of adipocytes, and we characterized their protein content. We find that immunopurified adipocyte caveolae have a relatively limited protein composition, and they lack the raft protein, flotillin, and insulin receptors. Immunogold labeling and electron microscopy of the adipocyte plasma membrane confirmed the lack of insulin receptors in caveolae. In addition to caveolins, the structural components of caveolae, their major protein constituents, are the semicarbazide-sensitive amine oxidase and the scavenger lipoprotein receptor CD36. Ongoing studies that employ mass spectral analysis focus on identifying additional protein components and the lipid complement associated with caveolae.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 脂肪细胞在生物体燃料代谢的胰岛素依赖性调节中发挥着重要作用，并且以与任何其他细胞类型一样高或更高的水平表达小凹。最近，有人提出胰岛素信号传导与小窝之间存在联系。然而，脂肪细胞小窝的研究相对较少，其内容物的特征也很少。借助新型单克隆抗体，我们开发了一种快速免疫分离脂肪细胞质膜小窝的方法，并表征了其蛋白质含量。我们发现免疫纯化的脂肪细胞小窝的蛋白质组成相对有限，并且它们缺乏筏蛋白、flotillin 和胰岛素受体。脂肪细胞质膜的免疫金标记和电子显微镜证实小窝中缺乏胰岛素受体。除小凹蛋白外，小凹蛋白的结构成分（其主要蛋白质成分）是氨基脲敏感的胺氧化酶和清道夫脂蛋白受体 CD36。正在进行的采用质谱分析的研究重点是识别与小窝相关的额外蛋白质成分和脂质补充。