Microbiome analysis in a mouse model of Alzheimers Disease (AD)

阿尔茨海默病 (AD) 小鼠模型中的微生物组分析

• 批准号: 10019246
• 负责人: Myriam Gorospe
• 金额: $ 13.52万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10019246
• 关键词: Affect; Aging; Alzheimer' s Disease; Alzheimer' s disease model; Brain; Chronic; Communities; DNA; Elderly; Genome; Goals; Health; Human; Immune system; Inflammaging; Inflammatory; Intestinal permeability; Mus; Patients; Prevotella; Process; Protocols documentation; Ribosomal RNA; Sampling; Serum; Shapes; Standardization; bacterial community; cytokine; gut microbiome; interest; metagenome; microbial; microbiome; microbiome analysis; microbiota; mouse model

We have investigated microbiota community involved in mouse and human fecal samples and recently started working on the serum samples. The most abundant bacterial taxa identified in mouse fecal samples were Bacteroidales, Clostridiales, Lactobacillales, Verrucomicrobiales. Also, there was significant difference in the microbial abundance between 2 month and 48 old mice. We also analyzed 18 human fecal samples from BLSA and found the genus Prevotella was significantly lower in aging (AIM 1a,b) (LGG, TGB, LEPS). We are actively working on standardizing the protocol extract and sequence from CSF and brain in mice (AIM 1c). We are also currently working on the impact of gut microbiome on pro-inflammatory cytokines and intestinal permeability (AIM 2).

我们已经研究了参与小鼠和人类粪便样品的微生物群落，并最近开始研究血清样品。 在小鼠粪便样品中鉴定出的最丰富的细菌分类群是杆菌，梭状芽胞杆菌，乳酸杆菌，verrucomicrobiales。 同样，在2个月至48只老鼠之间的微生物丰度也存在显着差异。 我们还分析了来自BLSA的18种人类粪便样品，发现衰老中的Prevotella属显着降低（AIM 1A，B）（LGG，TGB，LEPS）。 我们正在积极地致力于标准化小鼠中CSF和大脑的方案提取物和序列（AIM 1C）。 我们目前还在研究肠道微生物组对促炎细胞因子和肠道通透性的影响（AIM 2）。