STRUCTURAL STUDY OF ARABIDOPSIS CAD5 (CINNAMYL ALCOHOL DEHYDROGENASE 5) ENZYME

拟南芥CAD5（肉桂醇脱氢酶5）酶的结构研究

• 批准号: 7598075
• 负责人: CHULHEE KANG
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598075
• 关键词: Aldehydes; Anabolism; Anodes; Arabidopsis; Biological; Biology; Cinnamyl-alcohol dehydrogenase; Class; Classification; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Enzymes; Funding; Grant; Health; Human; Institution; Lignans; Lignin; Metabolic; Molecular; Pathway interactions; Plants; Play; Proteins; Reaction; Research; Research Personnel; Resolution; Resources; Role; Source; United States National Institutes of Health; cancer prevention; cofactor; pathogen

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Lignins, lignans and isoflavonoids represent very important class of phenylpropanoids. They play important roles in plant defense, helping hosts discriminate between biological pathogens and symbiotic non-pathogens. More importantly, various final metabolic products of phenylpropanoid pathway have important pharmacological roles, especially in cancer prevention. Despite the importance of these compounds in plant biology and in human health, systematic studies on the enzymes involved have not been done. We have studied one of the most important enzymes involved in phenylpropanoid biosynthesis pathways. Cinnamyl alcohol Dehydrogenase 5 (CAD5) enzyme depicts a class of NADPH-dependent enzymes catalyzing the reduction of various phenylpropenyl aldehyde derivatives. Generically, this reaction makes several monolignols, with the latter mainly being precursors of lignans. We have already obtained the diffraction data of CAD5 crystal (P41212) with 2.5 ¿ resolutions using a rotating anode (Rigaku, Raxis-IV). However, we couldn't perform the molecular replacement because there is no any similar protein solved. Therefore, MAD must be used. In addition to MAD data, the high resolution data and complex crystal (AtCAD5-cofactor/substrate/product) diffraction data are absolutely required for the studying of the exact mechanism of its unique stereospecific conversion of AtCAD5.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以出现在其他 CRISP 条目中 列出的机构是。 中心，不一定是研究者的机构。 木质素、木脂素和异黄酮类化合物是非常重要的一类苯丙素类化合物，它们在植物防御中发挥着重要作用，有助于宿主区分生物病原体和共生非病原体，更重要的是，苯丙素途径的各种最终代谢产物具有重要的药理学作用，特别是在癌症中。尽管这些化合物在植物生物学和人类健康中很重要，但我们尚未对所涉及的酶进行系统研究。肉桂醇脱氢酶 5 (CAD5) 酶描述了一类催化各种苯丙烯醛衍生物还原的酶，该反应主要产生几种木质素单体，后者是木脂素的前体。 CAD5晶体(P41212)的衍射数据，2.5 ¿使用旋转阳极（Rigaku，Raxis-IV）的分辨率但是，我们无法执行分子替换，因为没有任何类似的蛋白质被解析，因此，除了 MAD 数据外，还必须使用高分辨率数据和。为了研究 AtCAD5 独特的立体定向转换的确切机制，绝对需要复杂晶体（AtCAD5-辅因子/底物/产物）衍射数据。