FACS Core

流式细胞仪核心

基本信息

批准号：
7628048
负责人：
John David FARRAR
金额：
$ 12.8万
依托单位：
UT SOUTHWESTERN MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-06-01 至 2012-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7628048
关键词：
5-(6)-carboxyfluorescein diacetate succinimidyl ester Antibodies Arthritis Arts Autoimmune Process Biological Assay Cell Lineage Cell Separation Cell surface Cells Collaborations Compatible Cost Savings Custom Data Data Analyses Data Files Equipment Equipment and supply inventories Experimental Designs Financial compensation Flow Cytometry Fluorochrome Goals Housing Immunology Individual Label Laboratories Link Modeling Molecular Genetics Mus Myeloid Cells Numbers Online Systems Pathogenesis Phosphotransferases Pliability Population Preparation Principal Investigator Process Productivity Quality Control Rate Reagent Research Research Activity Research Infrastructure Research Personnel Resources Sampling Scientist Services Signal Transduction Signaling Molecule Sorting - Cell Movement Speed Staining method Stains Support of Research Surface System Systemic Lupus Erythematosus Techniques Testing Time Titrations Work base comparative cost cytokine data management density discount experience member novel programs

项目摘要

The Flow Cytometry Core will operate within the existing infrastructures of the Center for Immunology and the Harold C. Simmons Arthritis Research Center to support the research activities of Program investigators. The individual research programs proposed by these investigators have common requirements for reagents and services . The Core will consist of a Director, a Postdoctoral Research Scientist and a Research Technician. The Core will include a laboratory for preparation of samples and equipment in the Flow Cytometry Laboratory of the Immunology Center. A goal of this Core is to expand the polychromatic flow cytometry from cell surface lineage molecules to markers that include intracellular cytokines and kinases. The proposed activities of the Core will provide common and custom reagents and services that would not otherwise be available or economically feasible for the individual laboratories. The specific programs of the Core are: 1. Antibody Labeling and Resource Center 2. Quality Control Laboratory 3. Multiparameter Flow Cytometry Service Close collaboration between the Core and the principal investigators for the individual projects will be maintained in order to optimize their research productivity through utilization of Core reagents and services. In this way, redundancy will be avoided and research reagents will be provided at a reduced cost. This will allow for the centralization of certain techniques, such as high speed sorting, polychromatic flow cytometry and cytometric bead arrays, allowing for quality control and more rapid throughput.

流式细胞术核心将在该中心的现有基础设施内运行免疫学和 Harold C. Simmons 关节炎研究中心支持这项研究计划调查员的活动。这些项目提出的个人研究计划研究者对试剂和服务有共同的要求。核心将包括主任、博士后研究科学家和研究技术员。核心将包括一个流式细胞术实验室的样品制备实验室和设备免疫学中心。该核心的目标是将多色流式细胞术从细胞扩展到表面谱系分子到包括细胞内细胞因子和激酶在内的标记。拟议的核心的活动将提供通用和定制的试剂和服务，而这些试剂和服务不会否则对于各个实验室来说是可用的或经济上可行的。具体的核心计划是： 1. 抗体标记和资源中心 2. 质量控制实验室 3. 多参数流式细胞术服务各个项目的核心和主要研究人员之间的密切合作将进行维护，以便通过利用核心试剂来优化其研究生产力和服务。这样，就可以避免冗余，并且可以按时提供研究试剂。降低成本。这将允许某些技术的集中化，例如高速分拣，多色流式细胞术和细胞珠阵列，可进行质量控制等快速吞吐量。