Mechanisms of Brain Tumor Stem Cell Growth and Differentiation

脑肿瘤干细胞生长和分化的机制

• 批准号: 7594717
• 负责人: John Park
• 金额: $ 50.07万
• 依托单位: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7594717
• 关键词: Anaplastic astrocytoma; Animal Model; Astrocytes; Brain; Brain Neoplasms; Cell Differentiation process; Cells; Clinical; Data; Data Set; Gene Expression; Genetic; Glioma; Growth; Histologic; In Vitro; Malignant Glioma; Methods; Microarray Analysis; Molecular Profiling; Neoplastic Cell Transformation; Numbers; Oligodendroglia; Pathway interactions; Primary Neoplasm; Process; Reporting; Resected; Signal Pathway; Signal Transduction Pathway; Specimen; Stem cells; Therapeutic; Thinking; Tumor Stem Cells; Tumor-Derived; Ursidae Family; brain cell; cancer cell; cell growth; cell type; human NCOR1 protein; in vivo; neoplastic cell; nerve stem cell; oligodendroglioma; prevent; tumor; tumor growth; tumorigenesis

During this past fiscal year, we identified the N-CoR pathway as a regulator of tumor stem cell differentiation and reported pharmacologic ways to manipulate this pathway and inhibit tumor cell growth. We also continued our analysis of the microarray dataset that was initially reported in Lee et al., Cancer Cell (2006)9:391-403. This dataset contains gene expression data for freshly resected tumors and tumor stem cells derived from these tumors, both before and after differentiation. Analysis of the gene expression profiles revealed a number of specific intracellular signalling pathways that may be involved in the differentiation process of tumor stem cells. We will determine the importance of these pathways to tumorigenesis both in vitro and in vivo. Genetic and pharmacologic methods to disrupt the pathways relevant to tumor growth and spread will be explored.

在过去的财政年度中，我们将N-COR途径确定为肿瘤干细胞分化的调节剂，并报告了操纵这一途径并抑制肿瘤细胞生长的药理方法。 我们还继续对Lee等人，Cancer Cell（2006）9：391-403中报告的微阵列数据集进行分析。 该数据集包含分化前后源自这些肿瘤的新鲜切除的肿瘤和肿瘤干细胞的基因表达数据。 对基因表达谱的分析揭示了许多可能参与肿瘤干细胞分化过程的细胞内信号通路。 我们将确定这些途径在体外和体内对肿瘤发生的重要性。 将探索破坏与肿瘤生长和扩散相关的途径的遗传和药理方法。