Animal and Biostatistics Core

动物和生物统计学核心

• 批准号: 10374748
• 负责人: TODD E MORGAN
• 金额: $ 60.1万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-02-15 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10374748
• 关键词: Age; Animal Experiments; Animals; Aorta; Archives; Autopsy; Behavioral; Biological; Biostatistics Core; Blood; Body Composition; Body Temperature; Body Weight; Bone Marrow; Brain; Breeding; C-Peptide; Cardiac; Cell Aging; Cells; Cellular Stress; Cognitive; Cohort Analysis; Collection; Complement; Cysteine; Diet; Dose; Ensure; Environment; FRAP1 gene; Fasting; Freezing; Gases; Genetic; Genotype; Glucose; Goals; Growth; Heart; Hematopoietic; Hematopoietic System; Hematopoietic stem cells; Hippocampus (Brain); Histopathology; Hypothalamic structure; IGFBP1 gene; Immune; Incidence; Individual; Insulin; Insulin-Like Growth Factor I; Intervention; Kidney; Knock-out; Link; Liver; Longevity; Lyase; Mediator of activation protein; Metabolic; Microscopic; Mitochondria; Molecular; Molecular Profiling; Monitor; Mus; Natural regeneration; Neoplasms; Nervous system structure; Noise; Organ; Outcome; Pancreas; Pathology; Pathway interactions; Peptides; Pharmacology; Physical activity; Pilot Projects; Procedures; Production; Proteins; Protocols documentation; Records; Regulation; Rejuvenation; Reperfusion Injury; Research Personnel; Resistance; Sampling; Security; Skeletal Muscle; Specific qualifier value; Standardization; Stress; Testing; Tissue Banks; Tissues; Toxic effect; Transgenic Organisms; Work; age related; base; biological adaptation to stress; chemotherapy; cytokine; design; dietary restriction; experimental study; feeding; healthspan; histopathological examination; humanin; in vivo; liver ischemia; mimetics; mortality; mouse model; programs; protective effect; tissue regeneration

Abstract The Animal and Biostatistics Core will work directly with Project Leaders to design, plan, monitor and interpret all animal experiments, as well as, provide specific mouse models and standardized experimental protocols in support of the overall program's common goals: 1) understand how age and genetic background modulate the effects of fasting-mimicking diets (FMD) and protein restriction cycles (PRC) on longevity and healthspan, 2) study the mechanisms of FMD-, PRC-dependent cellular protection, regeneration and rejuvenation in the hematopoietic and nervous systems, 3) understand the link between FMD and PRC, GH-IGF-1, mTOR and humanin to test the hypothesis that this mitochondrial peptide is a PFC/PRC mediator with the potential to serve as a fasting/protein restriction mimetic, 4) study the regulation of the cysteine gamma lyase-dependent production of the gas H2S, negatively regulated by GH/IGF-1-mTOR, and test the hypothesis that H2S is a mediator of the protective effects of FMD on resistance to hepatic ischemia reperfusion injury and chemotherapy-induced toxicity to hematopoietic stem cells. The core provides an integrative framework for assessment of common outcomes across the individual projects needs. Aim 1. Design, plan and monitor animal experimental procedures as directed by Project Leaders. Studies are designed to achieve sufficient power/sensitivity to meet the stated objectives. All experimental procedures performed on mice are standardized and performed by Core B to ensure consistent assessment across all projects. These include: A) Longevity, B) Healthspan (Metabolic/Cognitive and Cellular/Molecular), C) Stress resistance and Mechanistic studies, D) Tissue collection/distribution. Aim 2. Coordinate animal use for program. All animals obtained or generated through Core B will be housed in the state-of-the-art vivarium facility in the Ray R Irani Hall at USC. Individual dedicated animal holding rooms for the exclusive use of either breeding, longevity or stress resistance will ensure consistency of biological environment, noise control and security across all studies.

抽象的 动物和生物统计核心将直接与项目负责人合作，设计、规划、监测和解释 所有动物实验，以及提供特定的小鼠模型和标准化实验方案 支持整个计划的共同目标：1) 了解年龄和遗传背景如何调节 模拟禁食饮食 (FMD) 和蛋白质限制周期 (PRC) 对寿命和健康寿命的影响，2) 研究FMD、PRC依赖性细胞保护、再生和复兴的机制 造血和神经系统，3) 了解 FMD 和 PRC、GH-IGF-1、mTOR 和 护脑素来测试这一假设：这种线粒体肽是一种 PFC/PRC 介质，具有潜在的 作为禁食/蛋白质限制模拟物，4) 研究半胱氨酸γ裂解酶依赖性的调节 GH/IGF-1-mTOR 负向调节 H2S 气体的产生，并检验 H2S 是一种气体的假设 FMD 对肝缺血再灌注损伤抵抗力的保护作用的中介 化疗引起的造血干细胞毒性。核心提供了一个综合框架 评估各个项目需求的共同结果。 目标 1. 按照项目负责人的指示设计、计划和监控动物实验程序。研究是 旨在实现足够的功率/灵敏度以满足既定目标。所有实验程序 对小鼠进行的实验是标准化的，并由 Core B 进行，以确保对所有小鼠进行一致的评估 项目。其中包括：A) 长寿，B) 健康寿命（代谢/认知和细胞/分子），C) 压力 阻力和机理研究，D) 组织收集/分布。 目标 2. 协调项目中的动物使用。通过Core B获得或生成的所有动物都将被安置在 南加州大学雷·R·伊拉尼大厅拥有最先进的动物饲养设施。单独的专用动物饲养室 专用于育种、长寿或抗逆性将确保生物学的一致性 所有研究中的环境、噪音控制和安全。