Stomate-based innate immunity against bacterial infection in Arabidopsis

拟南芥基于气孔的针对细菌感染的先天免疫

• 批准号: 7586692
• 负责人: SHENG YANG HE
• 金额: $ 36.74万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7586692
• 关键词: Abscisic Acid; Agriculture; Animals; Arabidopsis; Area; Bacteria; Bacterial Infections; Bacterial Toxins; Basic Science; Behavior; Biochemical; Biological Assay; Carbon Dioxide; Cells; Complex; Development; Effectiveness; Escherichia coli O157; Flagellin; Food Contamination; Food Poisoning; Future; Genetic; Goals; Health; Homeostasis; Hormones; Host Defense; Human; Humidity; Immune response; Infection; Knowledge; Left; Light; Lipopolysaccharides; Mediating; Microbe; Microscopy; Molecular; Molecular Genetics; Natural Immunity; Nitric Oxide; Output; Pathogenesis; Pattern; Peptides; Perception; Phosphotransferases; Plant Epidermis; Plant Growth Regulators; Plant Leaves; Plant Model; Plants; Production; Prophylactic treatment; Pseudomonas syringae; Public Health; Research; Role; Salicylic Acids; Signal Transduction; Signal Transduction Pathway; Source; Stomas; Stress; Surface; Testing; Time; Tomatoes; Vegetables; Virulence; Virulence Factors; Virulent; Water; base; cell type; coronatine; improved; movie; novel; pathogen; pathogenic bacteria; plant poison; prevent; receptor; response; ubiquitin-protein ligase; uptake

DESCRIPTION (provided by applicant): Innate immunity in animals and plants can be activated by highly conserved pathogen/microbe- associated molecular patterns (PAMPs/MAMPs), such as bacterial flagellin and lipopolysaccharides (LPSs). Recent studies provide evidence that plant stomata, pores in the epidermis of plants, function in innate immunity against the entry of bacteria into the plant. Specifically, stomata close in response to Escherichia coli O157:H7 and Pseudomonas syringae pv. tomato (Pst) DC3000 or bacterial PAMPs. Perception of PAMPs by guard cells, the pair of cells that forms the stomatal pore, requires a flagellin receptor as well as production and signaling of the plant hormones abscisic acid and salicylic acid. The virulent plant pathogen Pst DC3000 produces the phytotoxin coronatine (COR) to suppress stomatal closure as a virulence strategy. Collectively, these results suggest an important role of stomata in modulating the interactions of plants with plant and human pathogenic bacteria in the phyllosphere (the leaf surface). The long-term goal of this project is to elucidate the signal transduction pathway leading to stomate- based defense and to dissect the mechanism by which the bacterial toxin COR inhibits this novel host defense. The central hypotheses to be tested are that (i) stomate-based defense is an integral part of the plant's natural immune response to restrict the entry of bacteria, including human pathogenic bacteria, and (ii) to be successful, plant pathogens must rely on specific virulence factors or environmental conditions to suppress stomate-based defense. An integrative approach involving molecular genetics, microscopy, and biochemical and pathogenesis assays will be used. The proposed research will contribute to the fundamental knowledge of innate immunity and bacterial pathogenesis in plants and will increase our understanding of the molecular basis of plant-human pathogen interactions in the phyllosphere. Human pathogen contamination of fresh vegetables is a major public health concern and a potential bio-terrorist threat as a source of food poisoning. PUBLIC HEALTH RELEVANCE: This proposal describes basic research on the molecular basis of innate immunity of the model plant Arabidopsis to plant and human pathogenic bacteria. Food contamination with human pathogens is a major concern to public health. This research has the potential to provide a mechanistic understanding of plant defense and human pathogen contamination of fresh produce and to guide future development of appropriate prevention measures.

描述（由申请人提供）：动物和植物的先天免疫可以通过高度保守的病原体/微生物相关分子模式（PAMP/MAMP）激活，例如细菌鞭毛蛋白和脂多糖（LPS）。最近的研究提供的证据表明，植物气孔（植物表皮中的孔）在抵抗细菌进入植物的先天免疫中发挥着作用。具体来说，气孔因大肠杆菌 O157:H7 和丁香假单胞菌 pv. 的反应而关闭。番茄 (Pst) DC3000 或细菌 PAMP。保卫细胞（形成气孔的一对细胞）对 PAMP 的感知需要鞭毛蛋白受体以及植物激素脱落酸和水杨酸的产生和信号传导。剧毒植物病原体 Pst DC3000 产生植物毒素冠菌素 (COR)，以抑制气孔关闭作为毒力策略。总的来说，这些结果表明气孔在调节植物与叶际（叶面）中植物和人类病原菌的相互作用中发挥着重要作用。 该项目的长期目标是阐明导致基于气孔的防御的信号转导途径，并剖析细菌毒素 COR 抑制这种新型宿主防御的机制。要测试的中心假设是（i）基于气孔的防御是植物自然免疫反应的一个组成部分，以限制细菌（包括人类病原菌）的进入，以及（ii）要成功，植物病原体必须依赖抑制基于气孔的防御的特定毒力因素或环境条件。将使用涉及分子遗传学、显微镜、生化和发病机制测定的综合方法。拟议的研究将有助于植物先天免疫和细菌发病机制的基础知识，并将增加我们对叶际植物与人类病原体相互作用的分子基础的理解。新鲜蔬菜的人类病原体污染是一个主要的公共卫生问题，也是食物中毒的潜在生物恐怖威胁。 公共健康相关性：本提案描述了模式植物拟南芥对植物和人类致病菌先天免疫的分子基础的基础研究。人类病原体食品污染是公众健康的一个主要问题。这项研究有可能提供对新鲜农产品的植物防御和人类病原体污染的机制理解，并指导未来制定适当的预防措施。