MARSHALL U COBRE: GENOMICS COREP=C

马歇尔·U·科布雷：基因组学 COREP=C

• 批准号: 7610731
• 负责人: Donald Anthony Primerano
• 金额: $ 36.81万
• 依托单位: MARSHALL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610731
• 关键词: Area; Candidate Disease Gene; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Core Facility; DNA Sequence; DNA Sequence Analysis; Data; Data Analyses; Development; Experimental Designs; Funding; Gene Expression; Gene Expression Profiling; Genomics; Goals; Grant; Human Resources; Institution; Investigation; Malignant Neoplasms; Marshal; Measurement; Messenger RNA; Methods; Molecular; Pathway interactions; Polymerase Chain Reaction; Positioning Attribute; Reagent; Research; Research Personnel; Research Project Grants; Resources; Sequence Analysis; Signal Transduction; Source; Time; Training; United States National Institutes of Health; Universities; base; data management; expectation; experience; insight; instrumentation; novel; skills

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Each research project within this COBRE application relies heavily on gene expression analysis for the completion of their specific aims. The most common genomic approaches in this application are gene expression profiling by microarray, single mRNA measurement abundance by real-time PCR, and DNA sequencing and sequence analysis. Microarray experimentation and DNA sequencing require expensive instrumentation and reagents, sophisticated methods, and intense data management and analysis. These tasks are most efficiently executed by core facility personnel. The proposed COBRE projects cannot be completed without direct access to expert genomic technical and analytical support. Therefore, the objective of this application is to implement a Genomic Core Facility that will serve the specific needs of the COBRE and Marshall University investigations in the areas of microarray-based gene expression profiling, real-time PCR, automated DNA sequencing and DNA sequence analysis. Both the microarray manager and genomic data analyst will require substantial external training to keep pace with developments and provide useful analyses for the end users. The long-term goal of the Genomics Core Facility is to position COBRE investigators to discover novel molecular pathways in cancer development. We will enable the genomic research goals of the COBRE research projects by pursuing the following two specific aims: 1. Implement a Genomics Core Facility and develop Microarray Data Analysis capabilities. The Genomic C o re Facility will be co-directed by Drs Niles and Primerano who have complementary skills in experimental design and core management. Given the experience of the co-directors and presence of key personnel and instrumentation, we are well prepared to develop the Genomics Core Facility. It is our expectation that the Genomics Core Facility will enable COBRE and Marshall University investigators to identify candidate genes that are critically involved in cell signal transduction, confirm pattems of expression, and gain insights into cancer molecular biology.t?doF¿

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以出现在其他 CRISP 条目中 列出的机构是。 对于中心来说，它不一定是研究者的机构。 COBRE 应用程序中的每个研究项目都严重依赖于基因表达 完成其特定目标的分析。 该应用中的方法是通过微阵列、单个 mRNA 进行基因表达谱分析 通过实时 PCR、DNA 测序和序列分析测量丰度。 微阵列实验和 DNA 测序需要昂贵的仪器和 试剂、复杂的方法以及密集的数据管理和分析。 由核心设施人员最有效地执行拟议的 COBRE 项目。 如果不直接接触专家基因组技术和分析，就无法完成 因此，该应用程序的目标是实现基因组核心。 满足 COBRE 和马歇尔大学特定需求的设施 基于微阵列的基因表达谱、实时 PCR 等领域的研究 自动化 DNA 测序和 DNA 序列分析 两者都是微阵列管理器。 基因组数据分析师将需要大量的外部培训才能跟上 发展并为最终用户提供有用的分析 长期目标。 基因组学核心设施将帮助 COBRE 研究人员发现新的分子 我们将实现癌症发展的基因组研究目标。 COBRE 研究项目追求以下两个具体目标： 1. 实施 基因组学核心设施并开发微阵列数据分析能力。 基因组核心设施将由 Niles 博士和 Primerano 博士共同领导，他们曾 鉴于经验，实验设计和核心管理方面的互补技能。 联合董事以及关键人员和仪器的存在，我们很好 准备开发基因组学核心设施 我们期望基因组学。 核心设施将使 COBRE 和马歇尔大学的研究人员能够识别 关键参与细胞信号转导的候选基因，证实了 表达，并深入了解癌症分子生物学。