ShEEP Request for BD FACSAria Fusion Cell Sorting Flow Cytometer

ShEEP 请求 BD FACSAria 融合细胞分选流式细胞仪

• 批准号: 9361304
• 负责人: Nikhil C. Munshi
• 金额: --
• 依托单位: VA BOSTON HEALTH CARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-01-01 至 2017-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9361304
• 关键词: Aerosols; Affect; Alpha Cell; Biological; Biological Markers; Blood; Boston; Cell Culture Techniques; Cell Separation; Cell fusion; Cells; Collaborations; Deterioration; Disease; Equipment; Flow Cytometry; Functional disorder; Funding; Health; Healthcare Systems; Hematology; Immunology; Institution; Lasers; Legal patent; Malignant Neoplasms; Molecular; Molecular Analysis; Neurosciences; Neurosciences Research; Pathology; Phenotype; Population; Procedures; Proteins; Research; Research Personnel; Safety; Sampling; Schizophrenia; Scientist; Sheep; Sleep; Suspensions; System; Technology; Temperature; Time; Tissues; Transplantation; Transportation; Tumor Biology; Veterans; anticancer research; base; cell type; chemotherapy; cost; extracellular; mRNA Expression; research facility

This proposal describes a request for funds to purchase a BD FACSAria Fusion cell sorting flow cytometer with 4 lasers. In this proposal, we describe our needs for obtaining the equipment, its technical specifications, the potential for new collaborations and new avenues of research that will aid veterans, and a plan for the administration of the equipment. Cell sorting flow cytometers are widely used in a variety of research fields and the use of this equipment is specifically outlined and used in VA funded projects at the VA Boston Healthcare System (VABHS). A cell sorting flow cytometer does not exist in any VABHS research facility. However, since VABHS investigators currently must use affiliated institutions, there are added costs, time, and sample viability and procedures issues that limit our current abilities. Further, recent advances in technology are being applied in the diverse fields of research at the VABHS where cell sorting flow cytometry can be used to determine cell specific targets. The FACS equipment with 4 lasers will allow for cell sorting and analysis of up to 11 biomarkers simultaneously in cell population for an extremely diverse set of cells. The requested equipment has several capabilities that enhance its function and the safety of the operators. Having cell sorting flow cytometer capabilities will markedly enhance our abilities to understanding the pathophysiology of numerous disorders that affect veterans including cancer, sleep-related disorders, and schizophrenia. Possessing cell sorting flow cytometry capabilities at the VABHS will allow for new collaboration and attract and retain top-rate researchers. Also, this system is also contained in a protective hood that will enhance the protection of VA investigators.

该提案描述了购买BD FACSARIA融合细胞分类流的要求 带有4个激光器的细胞仪。在此提案中，我们描述了我们获得设备的需求， 技术规格，新合作的潜力以及新的研究途径 将帮助退伍军人，并制定设备管理计划。细胞分类流式细胞仪 广泛用于各种研究领域，该设备的使用是专门的 在VA波士顿医疗保健系统（VABHS）的VA资助项目中概述并使用。一个单元 在任何VABHS研究设施中，分类流式细胞仪均不存在。但是，自Vabhs 研究人员目前必须使用附属机构，增加成本，时间和样本 可行性和程序问题限制了我们当前的能力。此外，最近的进步 技术正在在细胞分类的VABHS的不同研究领域应用 流式细胞仪可用于确定细胞特定靶标。带4个的FACS设备 激光器将允许在细胞中同时分类和分析多达11个生物标志物 种群用于多样化的细胞集。请求的设备有几个 增强其功能和操作员安全的功能。具有细胞分类流 细胞仪能力将显着增强我们了解病理生理学的能力 在众多影响退伍军人的疾病中，包括癌症，与睡眠有关的疾病以及 精神分裂症。在VABHS上具有细胞分类流式细胞仪能力将允许 新的合作，吸引并保留顶级研究人员。另外，这个系统也是 包含在防护罩中，将增强VA调查人员的保护。