Route of Infection Shapes Immune Responses to Francisella

感染途径影响弗朗西斯菌的免疫反应

• 批准号: 7671889
• 负责人: JEFFREY Allen FRELINGER
• 金额: $ 13.26万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-15 至 2014-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7671889
• 关键词: Affect; Animal Model; Bacteria; Bacterial Genes; Breathing; Cell Communication; Cells; Complex; Dose; Fluorescence-Activated Cell Sorting; Francisella; Francisella tularensis; Generations; Genes; Goals; Human; Immune; Immune response; In Vitro; Infection; Infectious Skin Diseases; Insect Vectors; Intervention; Learning; Lung; Mediating; Microbe; Outcome; Pathogenesis; Pattern; Pharmaceutical Preparations; Process; Production; Route; Severity of illness; Shapes; Signal Transduction; Skin; Therapeutic Intervention; Tularemia; biodefense; chemokine; cytokine; immunoregulation; pathogen; research study; response

This proposal focuses on the earliest interactions of the F. tularensis the causative agent of tularemia with the host. Our hypothesis is that infection of particular cells in the lung results in a different pattern of host derived immunomodulatory molecules produced that shape the host innate and adaptive immune responses to benefit the pathogen. In this study we will identify the early cells infected in lung and skin infections. We will determine the molecules produced in the earliest cells following infection using a combination of fluorescence activated cell sorting and marked bacteria. By cell purification and in vitro infection we will learn how the impact the subsequent immune response. We will then determine the F. tularensis genes responsible, and their interactions with the host. Finally, we will determine mechanism for immune modulation.

该提案的重点是tularensis的最早相互作用。 主人。我们的假设是肺中特定细胞的感染导致宿主的不同模式 衍生的免疫调节分子产生了塑造宿主先天和适应性免疫反应的产生的免疫调节分子 受益于病原体。在这项研究中，我们将确定在肺和皮肤感染中感染的早期细胞。我们 将确定感染后最早细胞中最早细胞中产生的分子 荧光激活细胞分选和明显的细菌。通过细胞纯化和体外感染，我们将 了解随后的免疫反应的影响。然后，我们将确定tularensis基因 负责，及其与主持人的互动。最后，我们将确定免疫机制 调制。