Group Training in Product Development and Manufacturing

产品开发和制造团体培训

• 批准号: 7675670
• 负责人: Becky L Rivoire PhD
• 金额: $ 8.49万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7675670
• 关键词: Adjuvant; Animal Experiments; Animals; Antibiotics; Archives; Bacteria; Basic Science; Cells; Characteristics; Collaborations; Containment; Core Facility; Coupled; Coxiella; Coxiella burnetii; Development; Funding; Genome; Goals; Human Resources; Immunotherapy; Individual; Infection; Location; Lung; Montana; Mus; Nature; Organism; Phase; Positioning Attribute; Q Fever; Research; Research Personnel; Research Project Grants; Resources; Services; Source; System; Techniques; Therapeutic; Time; Tissues; Training; Universities; Vaccine Adjuvant; Vaccines; Work; biodefense; biosafety level 3 facility; design; immunoregulation; pathogen; product development; research study; tissue culture

Propagation and quantification of obligate intracellular pathogens are both labor-intensive and costly undertakings. These characteristics, coupled with C. burnetii's highly infectious nature and select agent designation, complicate the most basic research endeavors. However, because highly purified and enumerated C. burnetii is essential to the proposed research, a dedicated core facility with BSL 3 containment, highly trained technical personnel, and an archival system is proposed. Thus, the purpose of the Core B is to make available to each of the 4 Montana Coxiella projects expertise in the cultivation, purification, enumeration, and archiving of phase 1 and 2 Coxiella burnetii. The core will be located at Montana State University, the location of the BSL-3 facilities, and will be directed by Allen Harmsen of Montana State University. Collaborations among projects. Core B will provide services to each of the 4 research projects of the Montana Coxiella group as well as any other RMRCE investigator needing their service. These services will include making standardized inocula of phase 1 and 2 Coxiella burnetii as well as the Crazy strain of Coxiella, as well as the services of standardized Coxiella enumeration and archival of Coxiella organisms. In addition, the two part- time technicians are trained specifically in doing lung infections in mice in the BSL-3. When not engaged in their other activities, the Core B technicians will be available to assist the 4 Montana Coxiella projects on days they sacrifice mice since these time points are labor intensive. Our BSL3 SOP requires a "buddy system" and individuals are not allowed to work alone in the facility. It is much more economical to have two part time floating positions rather than for each of the individual research projects to fund full time technicians to cover these occasional, labor-intensive time points. Therefore, the core is integral and critical to the goals of each of the projects and required for consistency between projects so that results between projects can be directly compared. Core B will support the RMRCE Integrated Research Foci on Immunomodulation, Adjuvants and Vaccines (IRF 1) as well as Bacterial Therapeutics (IRF 2), and its resources will be utilized by RPs 1.1, 1.2, 1.3, and 2.4.

义务细胞内病原体的传播和量化既劳动密集型又昂贵 承诺。这些特征，以及C. burnetii的高度感染性和精选剂 名称，使最基本的研究工作变得复杂。但是，因为高度纯化和 列举的C. burnetii对于拟议的研究至关重要，这是一个专门的核心设施，该设施具有BSL 3 提出了遏制，训练有素的技术人员和档案系统。因此，目的 B核心是为蒙大拿州Coxiella的四个项目专业知识提供的核心， 1和2 Coxiella burnetii的纯化，枚举和归档。核心将位于 蒙大拿州立大学，BSL-3设施的位置，将由艾伦·哈姆森（Allen Harmsen）执导 蒙大拿州立大学。 项目之间的合作。核心B将为四个研究项目提供服务 蒙大拿州Coxiella Group以及其他需要服务的RMRCE调查员。这些服务将 包括制作1阶段和第2阶段Coxiella burnetii的标准化接种物以及疯狂的压力 考克斯菌，以及标准化的柯西埃拉枚举和库克西拉有机体的档案。在 此外，对两部兼职技术人员进行了专门的培训，以在BSL-3中的小鼠中进行肺部感染。 当不从事其他活动时，核心B技术人员将可以为4个蒙大拿州提供帮助 Coxiella在他们牺牲小鼠的日子里投影，因为这些时间点是劳动密集型的。我们的BSL3 SOP 需要一个“好友系统”，并且不允许个人在设施中独自工作。更多 具有两个兼职位置的经济性，而不是每个单个研究项目 为全职技术人员提供资金，以涵盖这些偶尔的劳动密集型时间点。因此，核心是 对每个项目的目标不可或缺和至关重要，并且需要在项目之间保持一致性所必需 项目之间的结果可以直接比较。 核心B将支持RMRCE集成的研究重点对免疫调节，佐剂和疫苗 （IRF 1）以及细菌疗法（IRF 2），其资源将由RPS 1.1、1.2、1.3和 2.4。