National Biomedical NMR Resource at Madison

麦迪逊国家生物医学 NMR 资源

• 批准号: 8316486
• 负责人: JOHN LUTE MARKLEY
• 金额: $ 19.6万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8316486
• 关键词: Area; Automation; Automobile Driving; Binding; Biological; Biological Factors; Biomolecular Nuclear Magnetic Resonance; Cell Extracts; Chemicals; Communities; Complex; Computer software; Consult; Data; Data Analyses; Data Collection; Databases; Deposition; Documentation; Educational workshop; Environmental Risk Factor; Evaluation; Feasibility Studies; Genetic; Goals; Health; Human; Human Resources; Instruction; Investigation; Knowledge; Label; Liquid Chromatography; Liquid substance; Magnetic Resonance; Manuscripts; Membrane Proteins; Methodology; Methods; Modeling; NMR Spectroscopy; Newsletter; Nuclear Magnetic Resonance; Nucleic Acids; Pharmaceutical Preparations; Physiologic pulse; Preparation; Process; Production; Proteins; Protocols documentation; Publications; Qualifying; RNA; Recovery; Research; Research Activity; Research Personnel; Resources; Sampling; Scientist; Screening procedure; Services; Site; Software Tools; Solutions; Structure; System; Technology; Tissues; Training; Validation; Visit; Work; base; biological systems; cost; data structure; design; experience; frontier; high throughput analysis; instrumentation; lectures; meetings; metabolomics; molecular dynamics; new technology; novel; posters; programs; protein complex; protein structure; research and development; research study; small molecule; software development; technology development; three dimensional structure; tool; web site

The National Magnetic Resonance Facility at Madison (NMRFAM) is a resource for biomolecular nuclear magnetic resonance (NMR) spectroscopy. NMRFAM aims to expand the frontiers of biomolecular NMR spectroscopy through resource technology and development programs in the important areas of (1) highthroughput determination of structures and functions of smaller proteins and RNA molecules, (2) technology for investigating the structure and dynamics of challenging systems, including complexes, membrane proteins, paramagnetic proteins, and larger RNA molecules, and (3) efficient approaches to metabolomics and natural product analysis. NMRFAM strives to be a model to the larger biological community for cutting-edge capabilities of NMR spectroscopy. With the goal of broadening the scope of its scientific activities, NMRFAM hosts distinguished visiting scientists working in areas related to its research technology and development projects. NMRFAM develops and disseminates advanced approaches that cover all steps in biomolecular NMR investigations and offers start-to-finish support for biomedical NMR investigations with assistance in one or more of the following steps: (1) strategy evaluation and experiment design, (2) sample preparaton, (3) feasibility studies, (4) data collection, (5) data analysis and structure determination, (6) data validation and deposition, and (7) manuscript preparation. NMRFAM provides researchers with resources matched to their particular needs in their search for new knowledge. A major goal is to develop methods for making these investigations faster and less costly as well as applicable to larger classes of proteins and nucleic acids of importance in human health. NMRFAM provides young investigators and experienced spectroscopists access to state-of-the-art instrumentation with support for multiple modes of data collection either as service or collaborative projects. Protocols, pulse sequences, software tools, and databases developed through NMRFAM's research activities are made available to the general scientific community. Users receive hands-on training at the center. As another means for training its user base and for disseminating its novel technology, NMRFAM conducts annual workshops and group training sessions. Additional mechanisms used to disseminate NMRFAM technology include quarterly newsletters, the NMRFAM website, software servers, a metabolomics database, presentations at meetings, and the publication of articles and reviews.

麦迪逊（NMRFAM）的国家磁共振设施是生物分子核的资源 磁共振（NMR）光谱。 NMRFAM旨在扩大生物分子NMR的边界 通过资源技术和开发计划的光谱学领域（1）高发射 确定较小蛋白质和RNA分子的结构和功能，（2）技术 用于调查具有挑战性系统的结构和动态，包括复合物，膜 蛋白质，顺磁蛋白和较大的RNA分子，以及（3）代谢组学和有效方法 天然产品分析。 NMRFAM致力于成为更大的生物界的典范 NMR光谱的能力。为了扩大其科学活动的范围， NMRFAM主持了与研究技术有关的领域的杰出访问科学家， 开发项目。 NMRFAM开发并传播了涵盖所有步骤的高级方法 生物分子NMR调查并为生物医学NMR调查提供首发支持 在以下一个或多个步骤中的帮助：（1）策略评估和实验设计，（2）样本 Preparaton，（3）可行性研究，（4）数据收集，（5）数据分析和结构确定，（6）数据 验证和沉积，以及（7）手稿准备。 NMRFAM为研究人员提供资源 在寻找新知识时与他们的特殊需求相匹配。一个主要目标是开发用于 使这些调查更快，成本较低，并且适用于较大类的蛋白质和 在人类健康中重要的核酸。 NMRFAM为年轻的调查人员和经验丰富 光谱学家访问最先进的仪器，并支持多种数据收集模式 作为服务或协作项目。开发的协议，脉冲序列，软件工具和数据库 通过NMRFAM的研究活动可供一般科学界提供。用户收到 中心动手训练。作为培训其用户群和传播小说的另一种手段 技术，NMRFAM进行年度研讨会和小组培训课程。使用的其他机制 传播NMRFAM技术包括季度新闻通讯，NMRFAM网站，软件服务器， 代谢组学数据库，会议上的演讲以及文章和评论的出版。

项目成果

Three-dimensional structure determined for a subunit of human tRNA splicing endonuclease (Sen15) reveals a novel dimeric fold.

人 tRNA 剪接核酸内切酶 (Sen15) 亚基的三维结构揭示了一种新的二聚体折叠。

• DOI: 10.1016/j.jmb.2006.11.024
• 发表时间: 2007
• 期刊: Journal of molecular biology
• 影响因子: 5.6
• 作者: Song,Jikui;Markley,JohnL
• 通讯作者: Markley,JohnL

[2Fe-2S]-ferredoxin binds directly to cysteine desulfurase and supplies an electron for iron-sulfur cluster assembly but is displaced by the scaffold protein or bacterial frataxin.

• DOI: 10.1021/ja401950a
• 发表时间: 2013-06-05
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Kim, Jin Hae;Frederick, Ronnie O.;Reinen, Nichole M.;Troupis, Andrew T.;Markley, John L.
• 通讯作者: Markley, John L.

Specialized Hsp70 chaperone (HscA) binds preferentially to the disordered form, whereas J-protein (HscB) binds preferentially to the structured form of the iron-sulfur cluster scaffold protein (IscU).

• DOI: 10.1074/jbc.m112.352617
• 发表时间: 2012-09-07
• 期刊: The Journal of biological chemistry
• 作者: Kim JH;Tonelli M;Frederick RO;Chow DC;Markley JL
• 通讯作者: Markley JL

Metabolic Reprogramming by 3-Iodothyronamine (T1AM): A New Perspective to Reverse Obesity through Co-Regulation of Sirtuin 4 and 6 Expression.

• DOI: 10.3390/ijms19051535
• 发表时间: 2018-05-22
• 期刊: International journal of molecular sciences
• 影响因子: 5.6
• 作者: Assadi-Porter FM;Reiland H;Sabatini M;Lorenzini L;Carnicelli V;Rogowski M;Selen Alpergin ES;Tonelli M;Ghelardoni S;Saba A;Zucchi R;Chiellini G
• 通讯作者: Chiellini G

Bacillibactins E and F from a Marine Sponge-Associated Bacillus sp.

• DOI: 10.1021/acs.jnatprod.0c01170
• 发表时间: 2021-01-22
• 期刊: Journal of natural products
• 影响因子: 5.1
• 作者: Wu Q;Throckmorton K;Maity M;Chevrette MG;Braun DR;Rajski SR;Currie CR;Thomas MG;Bugni TS
• 通讯作者: Bugni TS