APPLICATION OF PTMAP2 FOR PROTEIN POSTTRANSLATIONAL MODIFICATION ANALYSIS

PTMAP2 在蛋白质翻译后修饰分析中的应用

• 批准号: 8364316
• 负责人: YINGMING ZHAO
• 金额: $ 0.1万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364316
• 关键词: Biomedical Research; Cell physiology; Data Files; Escherichia coli Proteins; Funding; Grant; High Performance Computing; Mass Spectrum Analysis; National Center for Research Resources; Organism; Play; Post-Translational Protein Processing; Principal Investigator; Protein Databases; Proteins; Research; Research Infrastructure; Resources; Role; Source; Testing; United States National Institutes of Health; cost; genome-wide; protein function; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Posttranslational modifications (PTMs) play essential roles in protein function and cellular physiology. Unrestrictive identification of all types of PTMs using mass spectrometry is a powerful approach. Previous efforts have allowed us to successfully develop PTMap v2.0, a tool for genome-wide characterization of protein posttranslational modifications. Initial tests demonstrate that PTMap2 is capable to identify all types of PTMs in E. coli protein database from a single mass spectrometry data file. Here we propose to extend the application of PTMap2 tool to identify unknown PTMs in diverse cellular organisms.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 翻译后修饰 (PTM) 在蛋白质功能和细胞生理学中发挥着重要作用。使用质谱法无限制地鉴定所有类型的 PTM 是一种强大的方法。之前的努力使我们成功开发了 PTMap v2.0，这是一种用于蛋白质翻译后修饰的全基因组表征的工具。初步测试表明，PTMap2 能够从单个质谱数据文件中识别大肠杆菌蛋白质数据库中所有类型的 PTM。在这里，我们建议扩展 PTMap2 工具的应用来识别不同细胞生物中未知的 PTM。