The role of b-carotene in maternal-fetal nutrition

β-胡萝卜素在母婴营养中的作用

• 批准号: 8042693
• 负责人: Loredana Quadro
• 金额: $ 31.1万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-15 至 2013-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8042693
• 关键词: Address; All-Trans-Retinol; Biological; Birth; Blood; Blood Circulation; Carotene; Carotenoids; Cell Differentiation process; Child; Cleaved cell; Congenital Abnormality; Consumption; Data; Developed Countries; Development; Diet; Dietary Practices; Dose; Egg Yolk; Embryo; Embryonic Development; Enzymes; Etiology; Fetal Death; Fetus; Growth and Development function; Health; Homeostasis; Human; Incidence; Intake; Knowledge; Literature; Low Birth Weight Infant; Low Density Lipoprotein Receptor; Maternal Mortality; Metabolism; Micronutrients; Mothers; Mouse Strains; Mus; Mutation; Nutrient; Nutritional status; Organ; Oxygenases; Placenta; Plants; Population; Pregnancy; Process; Provitamin A Carotenoid; Public Health; Retinoids; Retinol Binding Proteins; Role; SR-B proteins; Source; Staging; Tissues; United States; Vitamin A; Vitamin A Deficiency; Vitamins; Yolk Sac; catalyst; cell growth; corticosterone methyl oxidase I; corticosterone methyl oxidase II; dietary supplements; embryo tissue; fetal; fetus nutrition; insight; lipid metabolism; lipoprotein lipase; malformation; mouse development; pi bond; uptake

DESCRIPTION (provided by applicant): The nutritional status of the mother is a key factor for the development and growth of the fetus. Vitamin A is an essential nutrient for the developing embryo, which depends on maternal circulating vitamin A (or retinoids) to fulfill its needs. Indeed, embryonic vitamin A-deficiency as well as -excess result in fetal malformations, representing a serious problem in both the developing world and the industrialized countries. Humans rely mainly on provitamin A carotenoids as a source of vitamin A. ¿-carotene is the best-characterized vitamin A precursor and the most abundant supply of this nutrient in the human diet. Two enzymes, ¿-carotene-15, 15'-oxygenase (CMO I) and ¿-carotene-9, 10'-oxygenase (CMO II), have been identified as catalysts for the cleavage of specific double bonds of ¿-carotene, resulting in the formation of vitamin A. At present, the role of ¿-carotene in maternal-fetal nutrition is not well understood. Evidences from the literature suggest that this carotenoid may be an important, localized source of vitamin A for the developing tissues. We plan to address this crucial biological question by using mice lacking both CMO I and retinol-binding protein (RBP), the sole specific carrier for retinol in the circulation. We will also use mice lacking low density lipoprotein receptor (LDLr) or class B scavenger receptor (SR-BI) or lipoprotein lipase (LPL) to investigate the role of these key players of the lipid metabolism in facilitating the maternal-fetal transfer of ¿-carotene. Three specific aims are proposed: (1) To identify factors that modulate the expression of CMO I and CMO II during mouse development. (2) To determine whether ¿-carotene is an alternative source of vitamin A for embryonic synthesis of retinoids. (3) To investigate the mechanisms of uptake and processing of ¿-carotene by the maternal-fetal barrier (i.e., placenta and yolk sac). These studies will ultimately expand our knowledge of maternal-fetal nutrition and dietary contribution to embryonic development, thus providing new insight into appropriate dietary practices during pregnancy. These studies have a significant impact on human health, since they aim at reducing the incidence of fetal malformations associated with an abnormal maternal intake of micronutrients, such as vitamins.

描述（由适用提供）：母亲的营养状况是胎儿发展和生长的关键因素。维生素A是发育中的胚胎的必不可少的营养素，它取决于母体循环维生素A（或类视黄素）以满足其需求。的确，胚胎维生素A缺乏症以及效果会导致胎儿畸形，这代表了发展中国家和工业化国家的严重问题。人类主要依靠类胡萝卜素作为维生素A的来源A。-胡萝卜素是人类饮食中这种营养素最丰富的先兆和最丰富的供应。两种酶，�-碳酸15、15'-氧合酶（CMO I）和�-arotene-9、10'-氧合酶（CMO II）已被鉴定为催化剂的催化剂，用于切断特定双键 - 陶瓷的特定双键，导致了载体的形成，在载体中，效果不足。理解。文献的证据表明，这种类胡萝卜素可能是发展组织的重要，局部的维生素A来源。我们计划通过使用缺乏CMO I和视黄醇结合蛋白（RBP）的小鼠解决这个关键的生物学问题，这是循环中视黄醇的唯一特异性载体。我们还将使用缺乏低密度脂蛋白受体（LDLR）或B级清除剂受体（SR-BI）或脂蛋白脂肪酶（LPL）的小鼠研究这些脂质代谢的这些关键参与者在促进母体及早转移中的主要参与者。提出了三个具体目标：（1）确定在小鼠发育过程中调节CMO I和CMO II表达的因素。 （2）确定�-胡萝卜素是否是维生素A的替代来源，用于类维生素的胚胎合成。 （3）研究孕产妇屏障（即胎盘和蛋黄囊）的吸收机理和–食用–食用的机制。这些研究最终将扩大我们对孕产妇营养和饮食贡献的了解，从而为胚胎发育提供了新的了解，从而为怀孕期间提供了适当的饮食习惯的新见解。这些研究对人类健康产生了重大影响，因为它们旨在减少与孕产妇摄入微量营养素（如维生素）相关的胎儿畸形事件。